A5020673590- Microtubule and mitosis dynamics
- Cancer-related Molecular Pathways
- Inhalation and Respiratory Drug Delivery
- Microencapsulation and Drying Processes
- Botany, Ecology, and Taxonomy Studies
- Advanced Breast Cancer Therapies
- Cancer Mechanisms and Therapy
- Hippo pathway signaling and YAP/TAZ
- Protist diversity and phylogeny
- Photosynthetic Processes and Mechanisms
- Synthesis and Biological Activity
- Animal Virus Infections Studies
- Animal and Plant Science Education
- Microbial Community Ecology and Physiology
- Genomics and Chromatin Dynamics
- Peroxisome Proliferator-Activated Receptors
- Genetic and Kidney Cyst Diseases
- Drug Solubulity and Delivery Systems
- DNA Repair Mechanisms
- Coleoptera Taxonomy and Distribution
- Chronic Lymphocytic Leukemia Research
- Marine Sponges and Natural Products
- RFID technology advancements
- Infectious Diseases and Mycology
- Ion Transport and Channel Regulation
Pfizer (United Kingdom)
2007-2016
George Mason University
2016
Pfizer (United States)
2014
University of Massachusetts Amherst
2012-2013
Twin Cities Orthopedics
2010
University of Minnesota
2010
University of Notre Dame
2010
Hormel (United States)
2010
Cardiff University
2007
Eli Lilly (United States)
2004-2005
A Spongosorites sp. collected during trawling operations off the southern coast of Australia returned new alkaloid dragmacidin E (3), structure which was secured by detailed spectroscopic analysis. Dragmacidin and its co-metabolite D (1) have been identified as potent inhibitors serine-threonine protein phosphatases.
We investigated why treatment of cells with dual aurora A and B kinase inhibitors produces phenotypes identical to inactivation B. found that in fact potently inhibit cellular activities both kinases, indicating bypasses mitosis. RNAi experiments further established indeed the requirement for leads polyploidy. Inactivation activates checkpoint BubR1 an B-dependent manner. Our results thus show is responsible mitotic arrest absence A.
In cultured mammalian cells, how dynein/dynactin contributes to spindle positioning is poorly understood. To assess the role of cortical in this process, we generated cell lines expressing localization and affinity purification (LAP)-tagged subunits from bacterial artificial chromosomes observed asymmetric dynein dynactin during mitosis. cells with asymmetrically positioned spindles, were both enriched at cortex distal spindle. NuMA, an upstream targeting factor, localized along a manner...
Understanding and controlling powder de-agglomeration is of great importance in the development dry inhaler (DPI) products. Dry dispersion laser diffraction measures particle size readily under controlled dispersing conditions, but has not been exploited fully to characterise inherent dispersibility. The aim study was utilise size-dispersing pressure titration curves cohesivity ease de-agglomeration. Seven inhaled drug/excipient powders (beclometasone dipropionate, budesonide, fluticasone...
Kinesin-5 is an essential mitotic motor. However, how its spatial-temporal distribution regulated in mitosis remains poorly understood. We expressed localization and affinity purification-tagged Eg5 from a mouse bacterial artificial chromosome (this construct was called mEg5) found to be tightly throughout mitosis. Fluorescence recovery after photobleaching analysis showed rapid turnover mitosis, which cannot accounted for by microtubule turnover. Total internal reflection fluorescence...
The synthonic modeling approach provides a molecule-centered understanding of the surface properties crystals. It has been applied extensively to understand crystallization processes. This study aimed investigate functional relevance formulation inhalation powders by assessing cohesivity three active pharmaceutical ingredients (APIs, fluticasone propionate (FP), budesonide (Bud), and salbutamol base (SB)) commonly used excipient, α-lactose monohydrate (LMH). is found that FP (−11.5 kcal/mol)...
During anaphase, overlapping antiparallel microtubules in the spindle interzone elongate and contribute to chromosome segregation. Kinesin-5 family members are required for elongation some cells, but other cases they restrict acting like a brake. To determine how kinesin-5 contributes mammalian we treated LLC-Pk1 epithelial cells with small molecule inhibitors of kinesin-5, Eg5, at anaphase onset measured rate extent pole separation using multidimensional tracking centrosomes expressing...
Abstract Background Non‐viral gene delivery vectors are multi‐component systems reflecting various functionalities required for effective cell transfection, including DNA condensation, promotion of membrane interactions and provision subcellular targeting through endosomal escape and/or nuclear delivery. Elements mediating these functions will clearly display inter‐dependency. In this study we sought to explore the relationship within non‐viral condensation localisation. Methods Binary,...
The protein kinase family represents an enormous opportunity for drug development. However, the current limitation in structural diversity of inhibitors has complicated efforts to identify effective treatments diseases that involve signaling pathways. We have identified a new class serine/threonine comprising aminoimidazo[1,2-a]pyridine nucleus. In this report, we describe first successful use aza-heterocycles generate potent cyclin-dependent kinases compete with ATP binding catalytic...
To investigate the agglomeration behaviour of fine (<5.0 μm) and coarse (>12.8 particle fractions salmeterol xinafoate (SX) fluticasone propionate (FP) by isolating aerodynamic size characterising their physicochemical re-dispersal properties.Aerodynamic fractionation was conducted using Next Generation Impactor (NGI). Re-crystallized control particles, unfractionated fractionated materials were characterized for size, morphology, crystallinity surface energy. Re-dispersal particles assessed...
When CHO cells are arrested in S-phase, they undergo repeated rounds of centrosome duplication without cell-cycle progression. While the increase is slow and asynchronous, number centrosomes these does rise with time. To investigate mechanisms controlling this duplication, we have S-phase for up to 72 h, coordinately inhibited new centriole formation by treatment microtubule poison colcemid. We find that such cells, pre-existing remain, a variable foci--containing alpha/gamma-tubulin centrin...
Premise of the study: We tested PCR amplification 91 low‐copy nuclear gene loci in taxa from Sapindales using primers developed for Bursera simaruba (Burseraceae). Methods and Results: Cross‐amplification these markers among 10 was related to their phylogenetic distance B. . On average, each Sapindalean taxon yielded product 53 regions (range: 16–90). Arabidopsis thaliana (Brassicales), by contrast, two. Single representatives Anacardiaceae Rutacaeae 34 26 products, respectively. Twenty‐six...