A5004636604- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Probiotics and Fermented Foods
- Antibiotic Resistance in Bacteria
- Genomics and Phylogenetic Studies
- Viral Infections and Immunology Research
- Free Radicals and Antioxidants
- Synthesis and Characterization of Heterocyclic Compounds
- Cancer Research and Treatments
- Parvovirus B19 Infection Studies
- Evolution and Genetic Dynamics
- Chemistry and Chemical Engineering
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Synthesis and Reactions of Organic Compounds
- CRISPR and Genetic Engineering
- Cancer Immunotherapy and Biomarkers
- RNA and protein synthesis mechanisms
- T-cell and Retrovirus Studies
- Milk Quality and Mastitis in Dairy Cows
- Transgenic Plants and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Bacteriophages and microbial interactions
- RNA Research and Splicing
- Effects and risks of endocrine disrupting chemicals
Washington University in St. Louis
2024-2025
University of Nebraska at Omaha
2017-2019
Engineered probiotics are an emerging platform for in situ delivery of therapeutics to the gut. Herein, we developed orally administered, yeast-based therapeutic system deliver next-generation immune checkpoint inhibitor (ICI) proteins directly gastrointestinal tumors. We engineered Saccharomyces cerevisiae var. boulardii (Sb), a probiotic yeast with high genetic tractability and innate anticancer activity, secrete "miniature" antibody variants that target programmed death ligand 1...
The prevalence of extended-spectrum beta-lactamases (ESBLs) among clinical isolates Escherichia coli has been increasing, with this spread driven by ESBL-encoding plasmids. However, the epidemiology ESBL-disseminating plasmids remains understudied, obscuring roles individual plasmid lineages in ESBL spread. To address this, we performed an in-depth genomic investigation 149 ESBL-like E. from a tertiary care hospital. We obtained high-quality assemblies for 446 plasmids, revealing extensive...
Enteroviral RNA genomes share a long, highly structured 5' untranslated region (5' UTR) containing type I internal ribosome entry site (IRES). The UTR is composed of stably folded domains connected by unstructured regions. Proper folding and functioning the underlies efficiency viral replication also determines virulence. We have characterized structure genomic from coxsackievirus B3 using selective 2'-hydroxyl acylation analyzed primer extension (SHAPE) base-specific chemical probes in...
Abstract Antimicrobial resistant pathogens such as Escherichia coli sequence type 131 (ST131) pose a serious threat to public health globally. In the United States, ST131 acquired multiple antimicrobial resistance (AMR) genes and rapidly grew its current high prevalence in healthcare settings. Notably, this coincided with introduction widespread use of antibiotics fluoroquinolones, suggesting AMR major driver ST131’s expansion. Yet, within ST131, there remains considerable diversity between...
Gastrointestinal colonization by Clostridioides difficile is common in healthcare settings and ranges presentation from asymptomatic carriage to lethal C. infection (CDI). We used a systems biology approach investigate why patients colonized with have range of clinical outcomes. Microbiota humanization germ-free mice fecal samples toxigenic carriers revealed spectrum virulence among clinically prevalent clade 1 lineages identified candidate taxa, including Blautia, as markers stable...
2-Iodoxybenzoic acid (IBX) oxidation of bisphenol A (BPA) is described. The selective production either the mono-o-quinone or di-o-quinone can be controlled by IBX stoichiometry. Isolated yields quinone were greater than 80%. Previous synthesis BPA-di-o-quinone using a large excess Fremy's salt produced only trace amounts product. In addition to o-quinone products, both mono- and dicatechols BPA synthesize in high yield isolated without chromatography. more stable catechols quantitatively...
Bisphenol A (BPA) has received considerable attention as an endocrine disrupting chemical and a possible substrate for genotoxic metabolites. BPA metabolism leads to formation of electrophilic o-quinones cable binding DNA other endogenous nucleophiles. We have structurally identified the products resulting from reaction bisphenol A-3,4-quinone (BPAQ) with N-acetylcysteine (NAC) glutathione (GSH). The major minor isomers are both result 1,6-conjugate addition produced almost instantly in high...
Precise transcriptional modulation is a key requirement for developing synthetic probiotics with predictably tunable functionalities. In this study, an expandable and transactivation system was constructed validated in probiotic yeast Saccharomyces boulardii. The use of nuclease-null Cas9 scaffold RNA (scRNA) directed regulation enabled under the control promoter S. A consisting scRNA target sequence core GAL7 region restricted interference from native galactose regulon. readily expanded by...
Gastrointestinal colonization by