A5023967626- DNA Repair Mechanisms
- Carcinogens and Genotoxicity Assessment
- Phytochemistry Medicinal Plant Applications
- Genetic factors in colorectal cancer
- Cancer therapeutics and mechanisms
- Essential Oils and Antimicrobial Activity
- Organoselenium and organotellurium chemistry
- Colorectal Cancer Treatments and Studies
- Fungal and yeast genetics research
- Piperaceae Chemical and Biological Studies
- Phytochemicals and Antioxidant Activities
- Selenium in Biological Systems
- Metal complexes synthesis and properties
- Genomics, phytochemicals, and oxidative stress
- Crystallization and Solubility Studies
- Phytochemistry and Biological Activities
- Pesticide Exposure and Toxicity
- X-ray Diffraction in Crystallography
- Synthesis and biological activity
- Chemotherapy-induced cardiotoxicity and mitigation
- Folate and B Vitamins Research
- Traditional and Medicinal Uses of Annonaceae
- Neuroscience and Neuropharmacology Research
- Bioactive Compounds and Antitumor Agents
- Synthesis and Biological Evaluation
Universidade Federal de Ciências da Saúde de Porto Alegre
2015-2024
Universidade Federal do Rio Grande do Sul
2010-2023
Discovery Air (Canada)
2021
Irmandade da Santa Casa de Misericórdia de São Paulo
2018
National Institute of Amazonian Research
2015
National Council for Scientific and Technological Development
2013
University of Health Sciences Antigua
2010-2012
Amazon (United States)
2012
Universidade Federal do Rio Grande
2010-2012
University of Health Sciences
2010-2011
The β-carboline alkaloids found in medical plants and a variety of foods, beverages cigarette smoke have range action various biological systems. In vitro studies demonstrated that these can act as scavengers reactive oxygen species. this paper, we report the vivo antioxidative properties aromatic (harmane, harmine, harmol) dihydro-β-carbolines (harmaline harmalol) studied by using Saccharomyces cerevisiae strains proficient deficient antioxidant defenses. Their antimutagenic activity was...
Never in mitosis A (NIMA)-related kinases (Nek) are evolutionarily conserved proteins structurally related to the Aspergillus nidulans mitotic regulator NIMA. Nek1 is one of 11 isoforms Neks identified mammals. Different lines evidence suggest participation response DNA damage, which also supported by interaction this kinase with involved repair pathways and cell cycle regulation. In report, we show that cells knockdown (KD) through stable RNA interference present a delay when treated...
Lesions with great loss of skin and extensive burns are usually treated heterologous grafts, which may lead rejection. Cell therapy mesenchymal stem cells is arising as a new proposal to accelerate the healing process. We tested consisting sodium carboxymethylcellulose (CMC) biomaterial, in combination adipose-derived (ADSCs), treat lesions an vivo rat model. This biomaterial did not affect membrane viability induced small transient genotoxicity, only at highest concentration (40 mg/mL). In...
Twenty pharmacists and nurses handling anti-neoplastic drugs in a hospital were monitored during working week, from Monday to Friday, the morning (only on Monday) afternoon (all days). Genotoxicity was analysed by comet assay micronucleus (MN) test, while oxidative stress serum thiobarbituric acid reactive substances (TBARS) measurements of antioxidant enzymes superoxide dismutase (Sod) catalase (Cat). The exposed workers presented increased DNA damage levels as compared controls. results...
We have investigated the chemical composition of Piper gaudichaudianum essential oil, as well its cytotoxic, mutagenic and genotoxic effects in V79 cells. The analyses showed that major compounds are (E)-nerolidol (22.4%), alpha-humulene (16.5%), (E)-caryophyllene (8.9%) bicyclogermacrene (7.4%). Dose-dependent cytotoxic were observed cells treated with oil by using clonal survival, 3-(4,5-dimethylthiazole-2-yl)-2,5-biphenyl tetrazolium bromide reduction assay (MTT) trypan blue exclusion...
Abstract 5‐Fluorouracil (5‐FU) is an antineoplasic drug widely used to treat cancer. Its cytotoxic effect has been principally ascribed the misincorporation of fluoronucleotides into DNA and RNA during their synthesis, inhibition thymidylate synthase (TS) by FdUMP (one 5‐FU active metabolites), which leads nucleotide pool imbalance. In present study, we compared ability induce apoptosis influence cell cycle progression in human colon SW620 adenocarcinoma cells regards genotoxic clastogenic...
Piper gaudichaudianum Kunth is used in popular medicine as anti-inflamatory and against liver disorders. One of the most studied components plant essential oil for which chemical analysis revealed (E)-nerolidol major compound. Recently, we have shown that P. possesses strong cytotoxic effects mammalian V79 cells. The aim this study was to analyze cytotoxicity mutagenicity nerolidol using Saccharomyces cerevisiae model study. Treatment XV185-14c N123 strains with led but did not induce...
Colorectal cancer (CRC) is prevalent worldwide, and treatment often involves surgery genotoxic chemotherapy. DNA repair mechanisms, such as base excision (BER) mismatch (MMR), may not only influence tumour characteristics prognosis but also dictate chemotherapy response. Defective MMR contributes to chemoresistance in colorectal cancer. Moreover, BER affects cellular survival by repairing damage a process that itself can disrupt metabolism. In this study, we characterized gene expression...
Cu(II) complexes bearing NNO-donor Schiff base ligands (2a, b) have been synthesized and characterized. The single crystal X-ray analysis of the 2a complex revealed that a mononuclear dinuclear co-crystallize in solid state. electronic structures are optimized by Density Functional Theory (DFT) calculations. monomeric nature 2b species is maintained solution. Antioxidant activities (1a, were determined vitro assays such as 1,1-diphenyl-2-picrylhydrazyl free radicals (DPPH.) 2,2′-azino-bis...
Bio-electrospraying (BES) is a technique used for the processing of cells and can be applied to tissue engineering. The association BES with scaffold production techniques has been shown an interesting strategy biomaterials homogeneously distributed in entire structure. Various studies have evaluated effects on different cell types. However, until present moment, no impact time mesenchymal stem (MSC). Therefore, aim this work was standardise parameters (voltage, flow rate, distance needle...