- Plant Taxonomy and Phylogenetics
- Plant and fungal interactions
- Agricultural pest management studies
- Legume Nitrogen Fixing Symbiosis
- Plant Disease Resistance and Genetics
- Turfgrass Adaptation and Management
- Genomics and Phylogenetic Studies
- Plant Pathogens and Fungal Diseases
- Nitrogen and Sulfur Effects on Brassica
- Genetic Syndromes and Imprinting
- Plant Virus Research Studies
- Ruminant Nutrition and Digestive Physiology
- Genetic Mapping and Diversity in Plants and Animals
- Soybean genetics and cultivation
- Chromosomal and Genetic Variations
- Genetic and Kidney Cyst Diseases
- Wheat and Barley Genetics and Pathology
- Plant Genetic and Mutation Studies
- Renal and related cancers
- Seed Germination and Physiology
- Plant pathogens and resistance mechanisms
- Horticultural and Viticultural Research
- Plant responses to water stress
- Botanical Research and Applications
- Genetics and Plant Breeding
The University of Adelaide
2018-2021
Commonwealth Scientific and Industrial Research Organisation
2014-2020
Agriculture and Food
2016-2020
AgriBio
2006-2014
La Trobe University
2009-2014
Health Sciences and Nutrition
2014
Plant Industry
2014
Dairy Australia (Australia)
2013
Agriculture Victoria
2006-2007
St George's Hospital
1999
The agriculturally important pasture grass tall fescue (Festuca arundinacea Schreb. syn. Lolium arundinaceum (Schreb.) Darbysh.) is an outbreeding allohexaploid, that may be more accurately described as a species complex consisting of three major (Continental, Mediterranean and rhizomatous) morphotypes. Observation hybrid infertility in some crossing combinations between morphotypes suggests the possibility independent origins from different diploid progenitors. This study aims to clarify...
Abstract Background The legume cowpea ( Vigna unguiculata L.) is extensively grown in sub-Saharan Africa. Cowpea, like many legumes has proved recalcitrant to plant transformation. A rapid transient leaf assay was developed for testing gene expression and editing constructs prior stable transformation, accelerate crop improvement. Results Attempts develop a protoplast system were unsuccessful. Leaflets from plants 3–4 weeks post-germination age selected establish Agrobacterium (Agro)...
Chloroplast genome sequences are of broad significance in plant biology, due to frequent use molecular phylogenetics, comparative genomics, population genetics, and genetic modification studies. The present study used a second-generation sequencing approach determine assemble the plastid genomes (plastomes) four representatives from agriculturally important Lolium-Festuca species complex pasture grasses (Lolium multiflorum, Festuca pratensis, altissima, ovina). Total cellular DNA was...
ABSTRACT A number of pasture and turf grass species form mutually beneficial symbiotic associations with endophytic fungal species. Within the fescue grasses, diploid meadow ( Festuca pratensis Huds.) interacts Neotyphodium uncinatum while allohexaploid tall arundinacea Schreb.) has been reported to associate coenophialum two other morphologically distinct taxa taxonomic groups 2 3 [ Fa TG‐2 TG‐3]). The evolutionary history hexaploid is complex, as part a group varying ploidy levels...
Abstract The availability of expressed sequence data derived from gene discovery programs enables mining for simple repeats (SSR), providing useful genetic markers crop improvement. These are inexpensive, require minimal labour to produce and can frequently be associated with functionally annotated genes. This study presents the development characterization 16 tags (EST)‐SSR Brassica juncea their cross‐amplification across species. Sixteen primer pairs were assessed polymorphism in all...
Cowpea ( Vigna unguiculata (L.) Walp) is an important legume crop for food security in areas of low-input and smallholder farming throughout Africa Asia. Genetic improvements are required to increase yield resilience biotic abiotic stress enhance cowpea performance. An integrated genomic gene expression data resource has the potential greatly accelerate breeding delivery novel genetic traits cowpea. Extensive resources have been absent from public domain; however, a recent early release...
Australian canola (Brassica napus L.) has been relatively isolated from the global gene pool and limited knowledge is available for genetic variability based on DNA profiling. In present study, diversity of recent cultivars was determined by simple sequence repeat (SSR) marker analysis. total, 405 individuals 48 varieties were genotyped with 18 primer pairs, resulting in 112 polymorphic features. The number features amplified each SSR pair varied 3 to 16. Analysis molecular variance (AMOVA)...
<ns4:p>Cowpea (<ns4:italic>Vigna unguiculata</ns4:italic> (L.) Walp) is an important legume crop for food security in areas of low-input and smallholder farming throughout Africa Asia. Genetic improvements are required to increase yield resilience biotic abiotic stress enhance cowpea performance. An integrated genomic gene expression data resource has the potential greatly accelerate breeding delivery novel genetic traits cowpea. Extensive resources have been absent from public domain;...
Application of apomixis, or asexual seed formation, in crop breeding would allow rapid fixation complex traits, economizing improved delivery. Identification apomixis genes is confounded by the polyploid nature, high genome complexity and lack genomic sequence integration with reproductive tissue transcriptomes most apomicts. A transcriptomic resource was developed for Hieracium subgenus Pilosella (Asteraceae) which incorporates characterized sexual, apomictic mutant apomict plants...
Abstract A collaborative Brassica rapa genome sequencing project is currently in progress to aid the identification of agronomically important traits species. As an initial stage, ends over 110 000 bacterial artificial chromosome clones were sequenced and mined for simple sequence repeats (SSRs). We present characterization 40 these SSRs their application napus . The markers screened against six species Arabidopsis , demonstrated reliable amplification, specificity, cross‐amplification...
Abstract The availability of expressed sequence data derived from gene discovery programs enables mining for simple repeats (SSR), providing useful genetic markers crop improvement. These are inexpensive, require minimal labour to produce and can frequently be associated with functionally annotated genes. This study presents the development characterization 24 tags (EST)‐SSR Brassica napus their cross‐amplification across species. show reliable amplification, genome specificity considerable...
Abstract Background Single nucleotide polymorphisms (SNPs) provide essential tools for the advancement of research in plant genomics, and development SNP resources many species has been accelerated by capabilities second-generation sequencing technologies. The current study aimed to develop use a novel bioinformatic pipeline generate comprehensive collection markers within agriculturally important pasture grass tall fescue; an outbreeding allopolyploid displaying three distinct morphotypes:...
White clover (Trifolium repens L.) is an outbreeding allotetraploid species and important forage legume in temperate grassland agriculture. Comparison of sub-genome architecture study nucleotide sequence diversity within allopolyploids provides insight into evolutionary divergence mechanisms, also necessary for the development whole-genome sequencing strategies. This aimed to evaluate degree between O P' sub-genomes white through BAC clones containing paired homoeoloci. The microsyntenic...
Due to the complex genetic architecture of perennial ryegrass, based on an obligate outbreeding reproductive habit, association-mapping approaches dissection offer potential for effective identification marker–trait linkages. Associations with genes agronomic characters, such as components herbage nutritive quality, may then be utilised accelerated cultivar improvement using advanced molecular breeding practices. The objective present study was evaluate presence associations a broad range...
Apomixis in plants generates clonal progeny with a maternal genotype through asexual seed formation. Hieracium subgenus Pilosella (Asteraceae) contains polyploid, highly heterozygous apomictic and sexual species. Within Hieracium, dominant genetic loci independently regulate the qualitative developmental components of apomixis. In H. praealtum, LOSS OF APOMEIOSIS (LOA) enables formation embryo sacs without meiosis PARTHENOGENESIS (LOP) fertilization-independent A locus required for endosperm...