A5086753834- Agricultural pest management studies
- Plant Reproductive Biology
- Legume Nitrogen Fixing Symbiosis
- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Photosynthetic Processes and Mechanisms
- Plant Pathogens and Resistance
- Seed Germination and Physiology
- Plant Genetic and Mutation Studies
- Plant Disease Resistance and Genetics
- Plant Taxonomy and Phylogenetics
- Ubiquitin and proteasome pathways
- Plant nutrient uptake and metabolism
- Seedling growth and survival studies
- Genetic and Environmental Crop Studies
- Plant Gene Expression Analysis
- Horticultural and Viticultural Research
- Microtubule and mitosis dynamics
- Soybean genetics and cultivation
- Enzyme Production and Characterization
- 14-3-3 protein interactions
- Lipid metabolism and biosynthesis
- Pasture and Agricultural Systems
Wageningen University & Research
2023-2024
Commonwealth Scientific and Industrial Research Organisation
2018-2020
Agriculture and Food
2018-2020
University of Zagreb
2008-2019
University of Regensburg
2012-2017
Abstract Germline and early embryo development constitute ideal model systems to study the establishment of polarity, cell identity, asymmetric divisions (ACDs) in plants. We describe here function MATH-BTB domain protein MAB1 that is exclusively expressed germ lineages zygote maize (Zea mays). mab1 (RNA interference [RNAi]) mutant plants display chromosome segregation defects short spindles during meiosis cause insufficient separation migration nuclei. After meiosis-to-mitosis transition,...
Abstract Background The legume cowpea ( Vigna unguiculata L.) is extensively grown in sub-Saharan Africa. Cowpea, like many legumes has proved recalcitrant to plant transformation. A rapid transient leaf assay was developed for testing gene expression and editing constructs prior stable transformation, accelerate crop improvement. Results Attempts develop a protoplast system were unsuccessful. Leaflets from plants 3–4 weeks post-germination age selected establish Agrobacterium (Agro)...
Apomixis results in asexual seed formation where progeny are identical to the maternal plant. In ovules of apomictic species Hieracium subgenus Pilosella, meiosis megaspore mother cell generates four megaspores. Aposporous initial (AI) cells form during most ovules. The sexual pathway terminates functional (FM) differentiation, when an enlarged AI undergoes mitosis aposporous female gametophyte. Then, mitotically programmed FM dies along with three other megaspores by unknown mechanisms....
Abstract Key message Multiple QTLs control unreduced pollen production in potato. Two major-effect co-locate with mutant alleles of genes homology to AtJAS, a known regulator meiotic spindle orientation. In diploid potato the gametes (2 n ) rather than haploid ( number chromosomes has been widely reported. Besides their evolutionary important role sexual polyploidisation, also have practical value for breeding as bridge between and tetraploid germplasm. Although early articles argued...
In most diploids the centromere-specific histone H3 (CENH3), assembly site of active centromeres, is encoded by a single copy gene. Persistance two CENH3 paralogs in species raises possibility subfunctionalization. Here we analysed both genes diploid dryland crop cowpea. Phylogenetic analysis suggests that gene duplication occurred independently during speciation Vigna unguiculata. Both functional variants are transcribed, and corresponding proteins intermingled subdomains different types...
MATH-BTB proteins are known to act as substrate-specific adaptors of cullin3 (CUL3)-based ubiquitin E3 ligases target protein for ubiquitination. In a previous study we reported the presence 31 genes in maize genome and determined regulatory role MAB1 during meiosis mitosis transition. contrast maize, there only 6 homologous model plant Arabidopsis, while this family has largely expanded grasses. Here, report phylogenetic analysis gene 9 land species including various mosses, eudicots, We...
MATH-BTB proteins are known to act as substrate-specific adaptors of CUL3-based E3 ligases in the ubiquitin proteasome pathway. Their BTB domain binds CUL3 scaffold and less conserved MATH targets a highly diverse collection substrate promote their ubiquitination subsequent degradation. In plants, significant expansion family occurred grasses. Here, we report analysis TaMAB2, protein transiently expressed at onset embryogenesis wheat. Due difficulties studying its role zygotes early embryos,...
Abstract The legume cowpea ( Vigna unguiculata , 2n=2x=22) has significant tolerance to drought and heat stress. Here we analysed manipulated centromere-specific histone H3 (CENH3) genes, aiming establish a centromere-based doubled-haploid method for use in genetic improvement of this dryland crop future. Cowpea encodes two functional CENH3 variants (CENH3.1 CENH3.2) pseudogenes. Phylogenetic analysis suggests that gene duplication occurred independently during the speciation V. related...
Abstract Molecular knowledge of pathways regulating seed formation in legumes, remains scarce. Thirteen isolated cell-type transcriptomes were developed, spanning temporal events male and female gametogenesis initiation, to examine involved cowpea formation. In situ hybridization confirmed localization silico identified cell-specific genes, verifying transcriptome utility. Cowpea Arabidopsis reproductive cells showed some conservation regulators enabling expression as genes promoters their...
Abstract The balanced segregation of homologous chromosomes during meiosis is essential for fertility and mediated by crossovers. A strong reduction crossovers leads to desynapsis, a process in which pairing abolished before metaphase I. This results random univalent the production unbalanced sterile gametes. However, if desynapsis combined with another meiotic alteration that restitutes first division, then uniform unreduced gametes, essentially composed non-recombinant homologs, are...
Apomixis in Hieracium praealtum follows a developmental pathway of apospory, where an unreduced embryo sac develops from somatic ovule cell without meiosis. The avoidance meiosis together with fertilization-independent seed formation leads to clonal progeny genetically identical the maternal plant. We have previously described initial steps aposporous H. and here, we cytologically observed more than 500 ovules focus on later stages maturation. Aposporous maturation is stochastic process...
Abstract In diploid potato the production of unreduced gametes with a (2n) rather than haploid (n) number chromosomes has been widely reported. Besides their evolutionary important role in sexual polyploidisation, also have practical value for breeding as bridge between and tetraploid germplasm. Although early articles argued monogenic recessive inheritance, genetic basis pollen remained elusive. Here, three full-sib populations were genotyped an amplicon sequencing approach phenotyped...