A5037408048- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Mast cells and histamine
- Blood Coagulation and Thrombosis Mechanisms
- Estrogen and related hormone effects
- Electrolyte and hormonal disorders
- Renin-Angiotensin System Studies
- Immunotherapy and Immune Responses
- Peptidase Inhibition and Analysis
- Ion channel regulation and function
- Ion Transport and Channel Regulation
- Neuroendocrine regulation and behavior
- Cancer, Hypoxia, and Metabolism
- Apelin-related biomedical research
- Urticaria and Related Conditions
- Histone Deacetylase Inhibitors Research
- Asthma and respiratory diseases
- Pregnancy and preeclampsia studies
- Receptor Mechanisms and Signaling
- Beetle Biology and Toxicology Studies
- Enzyme function and inhibition
- Pancreatic function and diabetes
- Immune Response and Inflammation
- HER2/EGFR in Cancer Research
- Autoimmune Bullous Skin Diseases
- Metabolism, Diabetes, and Cancer
Austral University of Chile
2015-2024
Centro de Estudios Científicos
2018-2020
University of Concepción
2006
Goethe University Frankfurt
2001-2005
Inserm
2004
La Jolla Institute For Molecular Medicine
2004
University of California, San Diego
2004
University of Michigan–Ann Arbor
2001-2002
Frequentis (Germany)
2001
Johannes Gutenberg University Mainz
2001
Abstract It has been proposed that the enhanced metabolic activity of tumor cells is accompanied by an increased expression facilitative hexose transporters (GLUTs). However, a previous immunohistochemical analysis GLUT1 in 154 malignant human neoplasms failed to detect isoform 87 tumors. We used 146 normal tissues and 215 samples reassess expression. A similar number were compare GLUT2–6 9. The classical GLUT1–5 different was confirmed, however, we unable GLUT2 pancreatic islet cells. GLUT6...
Free accessAbstractFirst published February 1995Probing for the bradykinin B2 receptor in rat kidney by anti-peptide and anti-ligand antibodies.C D Figueroa, C B Gonzalez, […], S Grigoriev, A Abd Alla, M Haasemann, K Jarnagin, W Müller-Esterl+4-4View all authors affiliationsVolume 43, Issue 2https://doi.org/10.1177/43.2.7822771
The Cl– channel ClC-2 is expressed in transporting epithelia and has been proposed as an alternative route for efflux that might compensate the malfunction of CFTR cystic fibrosis. There controversy concerning cellular membrane location ClC-2, particularly intestinal tissue. aim this paper to resolve by immunolocalization studies using tissues from knockout animals control, ascertaining sorting model epithelial cells exploring possible molecular signals involved targeting. was exclusively...
Abstract Kinins are biologically active peptides that powerful mediators of cellular inflammation. They mimic the cardinal signs inflammation by inducing vasodilatation and increasing vascular permeability pain. Neutrophils chemoattracted to sites several stimuli. However, evidence concerning chemotactic effect kinin has been contradictory. We analyzed B1 receptor agonists on neutrophils isolated from peripheral blood human healthy subjects. Chemotaxis was performed using migration under...
Intestinal fluid secretion is driven by apical membrane, cystic fibrosis transmembrane conductance regulator (CFTR)‐mediated efflux of Cl– that concentrated in cells basolateral Na + −K −2Cl– cotransporters (NKCC1). An absolute requirement for the parallel activation K channels which maintain a membrane potential sustains anion secretion. Both cAMP and Ca 2+ are intracellular signals intestinal The channel involved cAMP‐dependent has been identified as KCNQ1–KCNE3 complex, but identity...
Abstract Proinflammatory peptides known as kinins activate the human neutrophil and induce release of its primary tertiary granules. During activation degranulation, MMP-9 MPO are released into extracellular space to propagate inflammatory disorders. As kinin major participants in acute responses, G-protein-coupled B1R mediates chemotaxis neutrophils, we examined enzymes by agonist LDBK determined signaling pathways that may regulate this cellular effect. Cytochalasin-treated -untreated...
Kallikrein-related peptidases (KLKs) are a family of serine proteases that when dysregulated may contribute to neuroinflammation and neurodegeneration. In the present review article, we describe what is known about their physiological pathological roles with an emphasis on KLK6 KLK8, two KLKs highly expressed in adult central nervous system (CNS). Altered expression activity have been linked brain physiology development multiple sclerosis. On other hand, altered levels serum people affected...
GPER-1 is a novel membrane sited G protein-coupled estrogen receptor. Clinical studies have shown that patients suffering an receptor α (ERα)/GPER-1 positive, breast cancer lower survival rate than those who developed ERα-positive/GPER-1 negative tumors. Moreover, absence of improves the prognosis treated with tamoxifen, most used selective modulator to treat ERα-positive cancer. MCF-7 cells were chronically 1000 nM tamoxifen investigate its effect on protein expression, cell proliferation...
The principal function of the colon in fluid homeostasis is absorption NaCl and water. Apical membrane Na + channels, /H Cl − /HCO[Formula: see text] exchangers, have all been postulated to mediate entry into colonocytes. identity basolateral exit pathway for unknown. We previously demonstrated presence ClC-2 transcript guinea pig intestine. Now we explore more detail, tissue cellular distribution chloride channel distal by situ hybridization immunohistochemistry. patch-clamp technique was...
Angiogenesis gene therapy with human tissue kallikrein (hTK) has shown promise for ischemic disease. The present study was undertaken to (1) assess an optimal transfer modality, (2) clarify hTK angiogenic pathways, and (3) discount possible side effects.The transferred murine adductors by increasing doses of adenovirus (Ad.hTK). Heterologous protein production evaluated ELISA immunohistochemistry. Structural functional characteristics hTK-induced neovascularization were assessed. Muscular...
Current evidence suggests a functional and biochemical link between the renin kallikrein systems. The purpose of this work was to study localization along human nephron elucidate whether there exists an anatomical base for such interrelation. Serial sections kidney tissue were stained by immunocytochemical methods with antisera against kallikrein. Kallikrein immunostaining observed exclusively in segments distal lying cortical labyrinths forming arcades its portion. Consistently tubules...
-Bradykinin, a major vasodilator peptide, plays an important role in the local regulation of blood pressure, flow, and vascular permeability; however, cellular distribution bradykinin B(2) receptor cardiovascular system is not precisely known. Immunoblot analysis with anti-peptide antibody to or chemical cross-linkage [(125)I]Tyr(0)-bradykinin revealed band 69+/-3 kDa at varying intensity homogenates endothelium tunica media rat aorta endocardium. Immunostaining showed that abundant...