A5069849750- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Enzyme Structure and Function
- Amino Acid Enzymes and Metabolism
- Bacterial Genetics and Biotechnology
- Viral Infectious Diseases and Gene Expression in Insects
- Catalysis for Biomass Conversion
- Microbial Metabolites in Food Biotechnology
- Probiotics and Fermented Foods
- Food Quality and Safety Studies
- Enzyme Production and Characterization
- Gut microbiota and health
- Fungal and yeast genetics research
- biodegradable polymer synthesis and properties
- Pharmacological Effects of Natural Compounds
- Protein Hydrolysis and Bioactive Peptides
- Plant and animal studies
- Natural Products and Biological Research
- Bacterial biofilms and quorum sensing
- Biochemical and Molecular Research
- Antibiotic Resistance in Bacteria
- Nutrition, Health and Food Behavior
- Vibrio bacteria research studies
- Microbial Fuel Cells and Bioremediation
Suranaree University of Technology
2015-2025
University of Florida
2007-2009
Abstract Derivatives of Escherichia coli C were engineered to produce primarily succinate or malate in mineral salts media using simple fermentations (anaerobic stirred batch with pH control) without the addition plasmids foreign genes. This was done by a combination gene deletions (genetic engineering) and metabolic evolution over 2,000 generations growth‐based selection. After deletion central anaerobic fermentation genes ( ldhA , adhE ackA ), pathway for production remained as primary...
During metabolic evolution to improve succinate production in Escherichia coli strains, significant changes cellular metabolism were acquired that increased energy efficiency two respects. The energy-conserving phosphoenolpyruvate (PEP) carboxykinase ( pck ), which normally functions the reverse direction (gluconeogenesis; glucose repressed) during oxidative of organic acids, evolved become major carboxylation pathway for production. Both PCK enzyme activity and gene expression levels...
Derivatives of Escherichia coli C were previously described for succinate production by combining the deletion genes that disrupt fermentation pathways alternative products (ldhA::FRT, adhE::FRT, ackA::FRT, focA-pflB::FRT, mgsA, poxB) with growth-based selection increased ATP production. The resulting strain, KJ073, produced 1.2 mol per glucose in mineral salts medium acetate, malate, and pyruvate as significant co-products. KJ073 has been further improved removing residual recombinase sites...
The fermentative metabolism of glucose was redirected to succinate as the primary product without mutating any genes encoding native mixed-acid fermentation pathway or redox reactions. Two changes in peripheral pathways were together found increase yield fivefold: (i) increased expression phosphoenolpyruvate carboxykinase and (ii) inactivation phosphoenolpyruvate-dependent phosphotransferase system. These two net ATP production, pool available for carboxylation, production. Modest further...
Succinic acid is applied in many chemical industries which it can be produced through microbial fermentation using lignocellulosic biomasses. Mixed-waste office paper (MWOP) containing fibers enormously generated globally. MWOP recycled into toilet and cardboard, but the recovery process costly. The reuse of to alternatively produce succinic highly attractive. In this study, pretreatment MWOPs with 1% (v/v) H2SO4 at 121 °C for 20 min was found optimal. optimal conditions enzymatic hydrolysis...
β-Nicotinamide mononucleotide (NMN) has recently gained attention for a nutritional supplement because it is an intermediate in the biosynthesis of nicotinamide adenine dinucleotide (NAD+ ). In this study, we developed NMN synthesis by coupling two modules. The first module to culture E. coli MG1655 ▵tktA ▵tktB ▵ptsG metabolize xylose generate D-ribose medium. supernatant containing was applied second which composed EcRbsK-EcPRPS-CpNAMPT reaction synthesize NMN, that requires additional...
An optimization process with a cheap and abundant substrate is considered one of the factors affecting price production economical 2,3-Butanediol (2,3-BD). A combination conventional method response surface methodology (RSM) was applied in this study. The optimized levels pH, aeration rate, agitation speed, concentration (maltodextrin) were investigated to determine cost-effectiveness fermentative 2,3-BD by metabolically-engineered Klebsiella oxytoca KMS005. Results revealed that...
The viability and functionality of probiotics may be influenced by industrial production processes resulting in a decrease probiotic efficiency that benefit the health humans. This study aimed to investigate characteristics Bifidobacterium strains isolated from fecal samples healthy Thai infants. In present work, three local (BF014, BF052, BH053) belonging animalis showed great resistance against conditions simulating gastrointestinal tract. Among these, B. BF052 possessed considerable...
Crop residues can serve as low-cost feedstocks for microbial production of xylitol, which offers many advantages over the commonly used chemical process. However, enhancing efficiency xylitol fermentation is still a barrier to industrial implementation. In this study, effects oxygen transfer rate (OTR) (1.1, 2.1, 3.1 mmol O2/(L × h)) and initial xylose concentration (30, 55, 80 g/L) on Candida boidinii NCAIM Y.01308 medium were investigated optimised by response surface methodology,...