A5086023200- Diabetes and associated disorders
- Pancreatic function and diabetes
- Diabetes Management and Research
- Educational Innovations and Technology
- E-Learning and Knowledge Management
- Galectins and Cancer Biology
- Molecular Biology Techniques and Applications
- Digital literacy in education
- Immune Cell Function and Interaction
- Pancreatitis Pathology and Treatment
- Bacillus and Francisella bacterial research
- Tissue Engineering and Regenerative Medicine
- Pain Management and Opioid Use
- Adipokines, Inflammation, and Metabolic Diseases
- Ubiquitin and proteasome pathways
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- T-cell and B-cell Immunology
- Immunodeficiency and Autoimmune Disorders
- Viral gastroenteritis research and epidemiology
- Celiac Disease Research and Management
- Urinary Bladder and Prostate Research
- Communication and COVID-19 Impact
- Education and Digital Technologies
- Technology in Education and Healthcare
- Pancreatic and Hepatic Oncology Research
Universidad Complutense de Madrid
2021
Pontificia Universidad Católica del Ecuador
2018
Universitat Autònoma de Barcelona
1991-2009
Hospital Universitari Germans Trias i Pujol
1991-2003
Universitat de Barcelona
1997
We have used a reverse transcriptase–polymerase chain reaction (RT-PCR) protocol to examine the expression of cytokines in pancreases and islets patients with type I diabetes. detect significant increase level interferon (IFN)-α diabetic as compared control pancreases. In contrast, IFN-β was detected at comparable levels both groups, while IFN-γ three four one from individuals. The IFN-α cDNAs generated by RT-PCR were cloned sequenced determine which α-subtypes being expressed. found that...
Insulin-dependent diabetes mellitus (IDDM), in which only the pancreatic beta cells are destroyed by autoimmune response, is paradigm of organ-specific autoimmunity. As a result combination factors, number immunohistologic/cellular/molecular studies pancreas IDDM very limited. We report here conducted pancreata two patients: one newly diagnosed (case 1) and long standing 2). In case 1, we demonstrated presence morphologically normal viable without evidence viral infection. both cases...
CD14, a GPI-linked membrane protein, is component of the lipopolysaccharide (LPS) receptor complex, one pattern-recognizing receptors (PRR) expressed by myeloid lineage cells. Here we report that functionally linked toll-like molecules, TLR2 and TLR4, associated molecule MD-2 are in endocrine cells human pancreatic islets. CD14 expression islets was determined immunofluorescence staining tissue sections primary cultures, confirmed flow cytometry dispersed normal SV40-transformed islet...
Clinical transplantation of human islets has a disappointingly low rate success. We report here the identification possible causative factor: endotoxin present in collagenase preparations used to disperse pancreatic tissue before islet purification and transplantation. Supporting evidence includes (1) detection unexpectedly high levels most solutions currently digest pancreases; (2) demonstration that supernatants generated during separation are able induce inflammatory cytokines interleukin...
Understanding how T lymphocytes recognize beta-cell autoantigens is essential for the elucidation of pathogenesis insulin-dependent diabetes mellitus. The increased and ectopic expression HLA class I II molecules detected in human beta-cells may facilitate this interaction. T-lymphocyte recognition surface antigens also involves adhesion accessory molecules: intercellular molecule 1 (ICAM-1) lymphocyte function-associated antigen 3 (LFA-3). These not only allow cell contact but can provide...
A possible role of transporter associated with antigen processing (TAP)-l in the pathogenesis IDDM has been investigated by examining level TAP-1 expression islets pancreas and studying vitro effect interferon (IFN)-γ, IFN-α, tumor necrosis factor-α cultured islet cells. remarkable hyperexpression found endocrine cells (β non-β) islets, which constitutes first evidence this molecule target organ an autoimmune disease. correlated clearly HLA class I but only very partially II ectopic...
SUMMARY One of the paradoxes insulin-dependent diabetes mellitus is that destruction pancreatic islets’ endocrine cells restricted to insulin-producing β cells, whereas main autoantibodies, islet cell antibodies (ICA), are directed against all cells. GAD has recently been proposed as target humoral and cellular autoimmune attack islets, since in rat pancreas this enzyme was expressed only provided an explanation for specificity destructive process. The finding GAD-positive islets two...
Autoimmune thyroid diseases (AITD) and insulin‐dependent diabetes mellitus (IDDM) are two autoimmune syndromes of unknown etiology with common immune features. One is that the target cells, thyrocytes pancreatic islet beta cells respectively, hyperexpress several proteins encoded in HLA region: class I, II transporter associated antigen processing (TAP‐1); clinical course many aspects immunopathology are, however, quite different. Low‐molecular‐mass polypeptides 2 7 (LMP2 LMP7) proteasome...
Since their demonstration in 1975, ICSAs have been proposed as serological markers and pathogenic elements IDDM. are detected the sera of most newly diagnosed IDDM patients by indirect IFL that uses viable preparations rat islet or insulinoma cells substrate, but they also can be using human fetal cells. We tried to demonstrate 31 diabetic patients, including 6 positive samples on cells, which used natural target for first time: normal In spite different types these (i.e., freshly dispersed...
Human islet transplantation has a high rate of failure, often due to primary nonfunction, which suggests that islets are damaged during the processing pancreas. The preparation human for is still complex process requires large teams surgical and laboratory personnel. To overcome this problem, we have adopted use IBM 2991 COBE cell separator metrizamide/Ficoll density medium easy prepare. Twenty-seven pancreatic glands been processed using separator, 23 were purified in gradients 4 bovine...
Human islets from an adult subject with nesidioblastosis were isolated and used to perform in vitro studies. Isolated nesidioblastotic showed increased basal rate of insulin secretion (nesidioblastotic islets, 81.3 ± 6.4 vs. control cadaveric organ normal donors, 10.2 0.9 μU/islet/90 min) without any further release increasing glucose concentration. In addition, the pancreas contained more than 1,547.0 128.7 935.0 51.7 μU/islet, levels mRNA also higher those measured controls. Interestingly,...
Moodle es la plataforma online para el aprendizaje más popular en sistema universitario. En este artículo se expone una revisión bibliográfica (enfoque documental-descriptiva) con objetivo de determinar las características comprobar problemáticas su uso como herramienta didáctica. Se analizaron los siguientes ítems desde perspectiva del docente y estudiante: realidad ante tecnología educativa, capacidad agente educativo, resultados previos reacciones Moodle. El análisis permitió definir...