A5046421294- Retinal Diseases and Treatments
- Glaucoma and retinal disorders
- Retinal Development and Disorders
- Retinal Imaging and Analysis
- Traumatic Brain Injury and Neurovascular Disturbances
- Retinal and Optic Conditions
- Nitric Oxide and Endothelin Effects
- Retinopathy of Prematurity Studies
- Intraocular Surgery and Lenses
- Optical Imaging and Spectroscopy Techniques
- Corneal surgery and disorders
- Retinal and Macular Surgery
- Ocular and Laser Science Research
- Ophthalmology and Eye Disorders
- Neurological Disorders and Treatments
- Photoreceptor and optogenetics research
- Cerebral Venous Sinus Thrombosis
- Neonatal and fetal brain pathology
- Ocular Surface and Contact Lens
- Connexins and lens biology
- Renal and Vascular Pathologies
- Advanced Glycation End Products research
- Optical Coherence Tomography Applications
- Cardiac Ischemia and Reperfusion
- Antibiotics Pharmacokinetics and Efficacy
The University of Western Australia
2014-2023
Lions Eye Institute
2014-2023
Aravind Eye Hospital
2018
Australian Research Council
2009-2014
Centre for Sight
2010-2012
Obstetrics and Gynecology Hospital of Fudan University
2003
Queen Elizabeth II Hospital
1993
University of Hull
1993
Purpose.: To delineate morphometric and quantitative features of the capillary image derived from high-resolution fundus fluorescein angiography (FFA) consequently determine diagnostic value FFA for studying retinal circulation. Methods.: Retinal images obtained healthy young subjects using were compared with confocal scanning laser microscopic retinas age-matched human donors. Confocal acquired flatmount tissue after central artery cannulation, perfusion fixation, antibody labeling....
Purpose.: We investigated quantitatively the distribution of blood vessels in different neural layers human retina. Methods.: A total 16 donor eyes was perfusion-fixed and labeled for endothelial f-actin. Retinal eccentricity located 3 mm superior to optic disk studied using confocal scanning laser microscopy. Immunohistochemical methods applied whole-mount transverse sections were used colocalize capillary networks with neuronal elements. Capillary morphometry, diameter, density...
To quantify the distribution and morphometric characteristics of capillary networks in human perifovea. determine correlations between location neuronal subcellular compartments features regional layered retina.The perifoveal region, located 2 mm nasal to fovea, was studied 17 donor eyes. Novel micropipette technology used cannulate central retinal artery label microcirculation using a phalloidin perfusate. γ-synuclein, Goα, parvalbumin antibodies were also co-localize nerve fiber layer...
To relate the oxygen environment of retina to photoreceptor stability, protection, and function in P23H rat.Heterozygote P23H-3 (Line 3) rats were studied. Photoreceptor death rates assessed with TUNEL technique for detection fragmenting DNA, a developmental series from postnatal day (P)16 P105 (adult). In adult retinas, trophic factor status was immunohistochemistry, intraretinal O(2)-sensing electrodes, by flash-evoked, dark-adapted electroretinogram (ERG), recorded anesthetized...
purpose. To measure the intraretinal oxygen distribution and consumption in fovea, parafovea, inferior retina monkey eye determine influence of graded systemic hyperoxia. methods. Oxygen sensitive microelectrodes were used to tension as a function depth through anesthetized monkeys (n = 8) under normoxic hyperoxic conditions. rates avascular regions determined by fitting profiles established models. results. Under conditions, foveal area, reflected absence retinal capillaries predominantly...
To investigate the axonal cytoskeleton changes occurring in prelaminar region, lamina cribrosa, and postlaminar region of porcine optic nerve after an acute increase intraocular pressure (IOP) whether this corresponds with transport abnormalities.Six white Landrace pigs were used. The left eye IOP was elevated to 40 45 mm Hg for 6 hours, right maintained between 10 15 Hg. Rhodamine-beta-isothiocyanate (RITC) injected into vitreous each at beginning experiment, study transport. After...
To characterize the topography and cellular structure of macular microvasculature using a recently developed technique arterial cannulation, perfusion, fixation, staining human donor eyes.Sixteen eyes were used. The central retinal artery was cannulated perfused with Ringer's, then fixative, membrane permeabilizing, selected labeling solutions. immersion fixed, retina flat mounted for confocal microscopy. area, including foveola, fovea, parafovea, sampled. intracellular cytoskeleton vascular...
Differential responses to induced changes in systemic blood pressure (BP) at different layers of both the retinal and choroidal vasculature were observed, by monitoring localized PO2 as a function depth, retina choroid rat eye using oxygen-sensitive recessed microelectrodes. Visual electrophysiological localization microelectrode tip allowed oxygen distribution be related positions vascular beds choroid. Highly reproducible intraretinal profiles achieved. The relationship between BP was...