A5004125232- Retinal Development and Disorders
- Photoreceptor and optogenetics research
- Retinal Diseases and Treatments
- Laser Applications in Dentistry and Medicine
- Neuroinflammation and Neurodegeneration Mechanisms
- Anatomy and Medical Technology
- Ocular and Laser Science Research
- Retinopathy of Prematurity Studies
- Connexins and lens biology
- Neuroscience and Neuropharmacology Research
- Surgical Simulation and Training
- Mitochondrial Function and Pathology
- Chemical Reactions and Mechanisms
- Complement system in diseases
- Retinal and Macular Surgery
- Circadian rhythm and melatonin
- Chemical Reaction Mechanisms
- Innovations in Medical Education
- Retinal and Optic Conditions
- Synthesis and Reactions of Organic Compounds
- Glaucoma and retinal disorders
- Inorganic and Organometallic Chemistry
- Nitric Oxide and Endothelin Effects
- Asymmetric Synthesis and Catalysis
- Mosquito-borne diseases and control
Australian National University
2015-2025
National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”
2019
Vision Cooperative Research Centre
2012-2014
Australian Research Council
2011
Vision Australia
2011
Sichuan University
2010
West China Hospital of Sichuan University
2010
University of California, San Francisco
2010
Dean McGee Eye Institute
2010
University of Oklahoma
2010
Australian and New Zealand universities commenced a new academic year in February/March 2020 largely with "business as usual." The subsequent Covid-19 pandemic imposed unexpected disruptions to anatomical educational practice. Rapid change occurred due government-imposed physical distancing regulations from March that increasingly restricted anatomy laboratory teaching practices. Anatomy educators both these countries were mobilized adjust their approaches. This study on education disruption...
In this study, the hypothesis that near-infrared (NIR) light treatment (photobiomodulation) attenuates bright-light damage in albino rat retina was tested.Young adult Sprague-Dawley (SD) rats were raised dim (5 lux), cyclic and then exposed to bright (1000 continuous for 24 hours. The animals treated with 670-nm (9 J/cm(2)) an LED array before, during, or after exposure light. retinas examined function, structural changes, cell loss, markers of stress inflammation at 1 week month damaging...
Chemokine signalling is required for the homing of leukocytes during retinal inflammation, and associated with pathogenesis diseases such as age-related macular degeneration (AMD). Here, we explore role interleukin-1β (IL-1β) in modulating AMD-associated chemokines Ccl2, Cxcl1, Cxcl10 photo-oxidative damage, effect on both accumulation outer-retinal macrophages, death photoreceptors.Inhibition IL-1β expression was performed using either siRNA or antibody neutralisation, which intravitreally...
Monocyte infiltration is involved in the pathogenesis of many retinal degenerative conditions. This process traditionally depends on local expression chemokines, though roles these degenerating retina are unclear. Here, we investigate and situ localization broad chemokine response a light-induced model degeneration.Sprague-Dawley (SD) rats were exposed to 1,000 lux light damage (LD) for up 24 hrs. At time points during (1 hrs) following (3 7 days) exposure, animals euthanized retinas...
To relate the oxygen environment of retina to photoreceptor stability, protection, and function in P23H rat.Heterozygote P23H-3 (Line 3) rats were studied. Photoreceptor death rates assessed with TUNEL technique for detection fragmenting DNA, a developmental series from postnatal day (P)16 P105 (adult). In adult retinas, trophic factor status was immunohistochemistry, intraretinal O(2)-sensing electrodes, by flash-evoked, dark-adapted electroretinogram (ERG), recorded anesthetized...
Purpose.: To investigate the expression and localization of complement system mRNA protein in a light-induced model progressive retinal degeneration. Methods.: Sprague-Dawley (SD) rats were exposed to 1000 lux bright continuous light (BCL) for up 24 hours. At time points during (1–24 hours) after (3 7 days) exposure, animals euthanatized retinas processed. Differential genes at hours exposure was assessed using microarray analysis. Expression validated by quantitative PCR, selected...
Purpose.: To investigate the time course and localization of Ccl2 expression recruitment inflammatory cells associated with light-induced photoreceptor degeneration. Methods.: Sprague-Dawley (SD) rats were exposed to 1000 lux light for up 24 hours, after which some animals allowed recover in dim (5 lux) 3 or 7 days. During exposure light, euthanatized retinas processed. was assessed by qPCR, immunohistochemistry, situ hybridization at each point. Counts made perivascular monocytes/microglia...
Purpose: Complement system dysregulation is strongly linked to the progression of age-related macular degeneration (AMD). Deposition complement including C3 within lesions in atrophic AMD thought contribute lesion growth, although contribution local cellular sources remains unclear. We investigated role retinal microglia and macrophages activation lesions, models focal degeneration. Methods: Human donor retinas were labeled for expression via situ hybridization. Rats subject photo-oxidative...
To characterize the long-term spatiotemporal features of light-mediated retinal degeneration.Sprague-Dawley rats were exposed to 1000 lux for 24 h, then kept in dim light (5 lux), up 56 days. Animals killed at 0, 3, 7, 28, and days post-exposure, retinas prepared immunohistochemistry. Outer nuclear layer (ONL) thickness TUNEL labeling used quantify photoreceptor death. Antibodies opsins, glial fibrillary acidic protein (GFAP), fibroblast growth factor-2 (FGF-2), ED1 assess retina.At 0 we...
Abstract Glial cells play an important role in the maintenance of normal structure and function neural components central nervous system. The Müller are one macroglial elements retina their wide‐ranging roles responsible for protection proper functioning photoreceptors. In present study, we aimed to test effects pretreatment with 670 nm red light on light‐induced model retinal degeneration. Adult Sprague–Dawley albino rats were treated light, from LED source prior exposure bright (1000 lux)...
Complement activation is associated with the pathogenesis of age-related macular degeneration (AMD). We aimed to investigate whether 670-nm light treatment reduces propagation complement in a light-induced model atrophic AMD. Sprague–Dawley (SD) rats were pretreated 9 J/cm2 for 3 minutes daily over 5 days; other animals sham treated. Animals exposed white (1,000 lux) 24 h, after which kept dim (5 7 days. Expression genes was assessed by quantitative polymerase chain reaction (qPCR), and...
We sought to determine the role and retinal cellular location of microRNA-124 (miR-124) in a neuroinflammatory model degeneration. Further, we explored anti-inflammatory relationship miR-124 with predicted messenger RNA (mRNA) binding partner, chemokine (C-C motif) ligand 2 (Ccl2), which is crucially involved inflammatory cell recruitment damaged retina.Human AMD donor eyes photo-oxidative (PD) mice were labeled for expression using situ hybridization. PDGFRa-cre RFP used Müller isolation...
In 2011, a consensus report was produced on technology-enhanced assessment (TEA), its good practices, and future perspectives. Since then, technological advances have enabled innovative practices tools that revolutionised how learners are assessed. this updated consensus, we bring together the potential of technology ultimate goals learner attainment, faculty development, improved healthcare practices.As material for report, used scholarly publications TEA in both HPE general higher...
IntroductionTo investigate the validity of using 670nm red light as a preventative treatment for Retinopathy Prematurity in two animal models oxygen-induced retinopathy (OIR). Materials and MethodsDuring post exposure to hyperoxia, C57BL/6J mice or Sprague-Dawley rats were exposed 3 minutes day (9J/cm2). Whole mounted retinas investigated evidence vascular abnormalities, while sections neural retina used quantify levels cell death TUNEL technique. Organs removed, weighed independent...