A5034194433- Growth Hormone and Insulin-like Growth Factors
- Metabolism, Diabetes, and Cancer
- Diabetes Treatment and Management
- Bone Metabolism and Diseases
- Pancreatic function and diabetes
- Protease and Inhibitor Mechanisms
- Bone health and osteoporosis research
- Bone health and treatments
- Connective tissue disorders research
- Diabetes and associated disorders
- Diabetes Management and Research
- Blood Coagulation and Thrombosis Mechanisms
- Genetic Syndromes and Imprinting
- Vitamin D Research Studies
- Craniofacial Disorders and Treatments
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Sexual Differentiation and Disorders
- Peptidase Inhibition and Analysis
- Chronic Kidney Disease and Diabetes
- Parathyroid Disorders and Treatments
- Adipose Tissue and Metabolism
- Regulation of Appetite and Obesity
- Gestational Diabetes Research and Management
- Dialysis and Renal Disease Management
- Pituitary Gland Disorders and Treatments
University of Kentucky
1999-2022
University of Kentucky HealthCare
2021
Arkansas Children's Hospital
2003-2015
University of Arkansas for Medical Sciences
2006-2015
Duke University
1984-1997
Duke Medical Center
1988-1997
Duke University Hospital
1988-1997
University of North Carolina at Chapel Hill
1988-1990
The Ohio State University
1980-1987
University of Illinois Chicago
1987
The effects of type 1 diabetes on de novo bone formation during tibial distraction osteogenesis (DO) and intact trabecular cortical were studied using nonobese diabetic (NOD) mice comparably aged nondiabetic NOD mice. Diabetic received treatment with insulin, vehicle, or no a 14-day DO procedure. Distracted tibiae analyzed radiographically, histologically, by microcomputed tomography (μCT). Contralateral μCT. Serum levels osteocalcin, cross-linked C-telopeptide I collagen measured. Total new...
Vitamin D deficiency is an increasingly recognized comorbidity in patients with both type 1 (T1D) and 2 diabetes, particularly associated the presence of diabetic nephropathy.Because we have previously reported enhanced excretion megalin urine T1D microalbuminuria, hypothesized that concurrent urinary loss ligand, vitamin binding protein, might contribute mechanistically to deficiency.Examining a study cohort 115 subjects T1D, aged 14-40 yr, along 55 age-matched healthy control subjects,...
Insulin-like growth factor (IGF)-binding protein-5 (IGFBP-5) is uniquely regulated throughout MC3T3-E1 osteoblast differentiation: IGFBP-5 first detectable in conditioned medium (CM) of replicating preosteoblasts (day 5); levels peak between culture days 8-12, then decline to almost undetectable mature cultures (> day 18) despite the persistence messenger RNA. These observations suggest that concentrations may be by posttranslational mechanisms. To determine whether proteolysis contributes...
Typically, growth failure in Turner syndrome (TS) begins prenatally, and height sd score (SDS) declines progressively from birth.This study aimed to determine whether GH treatment initiated before 4 yr of age girls with TS could prevent subsequent failure. Secondary objectives were identify factors associated response, outcome be predicted by a regression model using these factors, assess the safety this young cohort.This was prospective, randomized, controlled, open-label, multicenter...
OBJECTIVE—Dysregulation of matrix metalloproteinase (MMP)-2 may contribute pathologically to the development diabetes complications, including diabetic retinopathy and coronary peripheral arterial disease. Our objective was explore whether systemic MMP-2 dysregulation could be demonstrated in type 1 determine how concentration relates disease status. RESEARCH DESIGN AND METHODS—In this cross-sectional study, concentrations activity were measured plasma timed urine samples from 93 50 healthy...
Type 1 diabetes mellitus is associated with a number of disorders skeletal health, conditions that rely, in part, on dynamic bone formation. A mouse model distraction osteogenesis was used to study the consequences streptozotocin-induced and insulin treatment formation osteoblastogenesis. In diabetic mice compared control mice, new decreased, adipogenesis increased around, respectively, gaps. Although restored levels observed nondiabetic it failed significantly decrease adipogenesis....
OBJECTIVE Proteinuria is the hallmark of diabetic nephropathy; yet, glomerular histology does not fully explain mechanisms contributing to proteinuria. Our objective was identify proteins in urine individuals with type 1 diabetes and microalbuminuria that might implicate a mechanistic pathway operative RESEARCH DESIGN AND METHODS Using GeLC/MS platform proteomics approach, we compared proteome from 12 healthy nondiabetic individuals, subjects yet normal urinary albumin excretion rates, (type...
Objective To adapt and pilot test a multicomponent motivational intervention that includes family-based contingency management (CM) for adolescents with poorly controlled type 1 diabetes. Methods A total of 17 adolescents, age 12–17 years (M = 14.8, SD 1.5), diabetes (duration M 6.2 years, 4.5) mean HbA1c 11.6% (SD 2.5%) were enrolled. Adolescents their parents received 14 weeks interviewing, clinic-based CM, parent-directed CM targeted increased blood glucose monitoring (BGM). Results...
Insulin-like growth factor binding protein-3 (IGFBP-3) is degraded by a cation-dependent protease(s) present in the serum of late gestation rats. Proteolysis IGFBP-3 results an increase IGF-I clearance and possibly IGF bioavailability. Based on our previous findings that matrix metalloproteinases (MMPs) degrade fibroblast conditioned media, we hypothesized MMPs might be involved degradation rat pregnancy serum. In study, demonstrate tissue inhibitor (TIMP-1), specific all MMPs, inhibited...
Insulin resistance is associated with a proinflammatory state that promotes the development of complications such as type 2 diabetes mellitus (T2DM) and atherosclerosis. The metabolic stimuli initiate propagate cytokine production cellular origin cytokines in insulin have not been fully elucidated. Circulating monocytes show signs enhanced inflammation obese, resistant subjects are thus potential source production. specific, circulating factors might stimulate monocyte poorly characterized....
OBJECTIVE: To study the effects of 12 weeks cotherapy with recombinant human IGF-I (rhIGF-I) and insulin on glycemic control in patients type 1 diabetes. RESEARCH DESIGN AND METHODS: The population consisted 223 who ranged age from 11-66 years were randomized a double-blind to receive treatment twice-daily subcutaneous injections placebo (n = 54), or rhIGF-I at dose (A.M/P.M) 40/40 micrograms/kg 56), 80/40 57), 80/60 while continuing standard therapy. Patients instructed test blood glucose...
MC3T3-E1 murine osteoblasts produce insulin-like growth factor (IGF)-binding protein-4 (IGFBP-4)-degrading proteinase activity, which is inhibited by IGFBP-3 and a highly basic, C-terminal domain of IGFBP-3. Of all the other five IGFBPs, IGFBP-5 -6 share highest degree homology with this IGFBP-3; therefore, we investigated whether these two IGFBPs inhibit IGFBP-4 degradation. Both IGFBP-6 degradation 125I-IGFBP-4 MC3T3-E1-conditioned media, their inhibitory effects are variably reversed...
DO was used in an aged mouse model to determine if systemically and/or locally administered rhIGF-I improved osteoblastogenesis and new bone formation. Local systemic treatment increased However, only delivery produced measurable concentrations of the circulation.Human rodent research supports a primary role for IGF-I Significant roles both endocrine paracrine/autocrine have been suggested normal We assessed, using distraction osteogenesis (DO), impact continuous administration recombinant...
Recent studies suggest a role for insulin-like growth factor I (IGF-I) in the regulation of hormone release from placental, gonadal, and pituitary tissues. To examine whether IGF-I may also regulate PRL human decidual tissue, we have investigated effect recombinant on monolayer cultures cells exposed to up 4 days. (10-1000 ng/ml) stimulated sustained dose-dependent increase (half-maximal concentration, 25 beginning 48 h after initial exposure, but had no intracellular content. The amounts...