A5059257287- Virus-based gene therapy research
- Glaucoma and retinal disorders
- Nerve injury and regeneration
- Herpesvirus Infections and Treatments
- Digital Imaging for Blood Diseases
- Tissue Engineering and Regenerative Medicine
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer, Hypoxia, and Metabolism
- Inflammatory mediators and NSAID effects
- Extracellular vesicles in disease
- Silk-based biomaterials and applications
- Cellular transport and secretion
- Chemical Synthesis and Reactions
- Retinal Diseases and Treatments
- Multicomponent Synthesis of Heterocycles
- Peroxisome Proliferator-Activated Receptors
- Biotin and Related Studies
- Microtubule and mitosis dynamics
- Xenotransplantation and immune response
- Receptor Mechanisms and Signaling
- Viral gastroenteritis research and epidemiology
- Asymmetric Synthesis and Catalysis
- Ubiquitin and proteasome pathways
Dana-Farber/Boston Children's Cancer and Blood Disorders Center
2024
Harvard University
2024
Boston Children's Hospital
2024
University of Minnesota Medical Center
2024
Massachusetts General Hospital
2024
University of Wisconsin–Madison
2012-2018
Bluebird Bio (United States)
2016
Gazi University
2014
Seattle University
2008
Gene therapy with elivaldogene autotemcel (eli-cel) consisting of autologous CD34+ cells transduced lentiviral vector containing
Domino reactions were designed to allow the byproduct of an upstream reaction be internally recycled catalyze a downstream in one-pot tandem sequence. Nitroarene reduction by In(0) generates amine and In (III) byproducts. Addition aldehyde followed Danishefsky's diene or silyl ketene acetal provides access dihydropyridin-4-ones beta-amino esters, respectively, yields that are comparable superior reported stepwise reactions.
Purified Clostridium botulinum exoenzyme C3 transferase (C3) effects on the actin cytoskeleton in human trabecular meshwork cells (HTM) and outflow facility response monkey organ-cultured anterior segments (MOCAS) were determined presence or absence of viral vectors.Human adenovirus type 5 (AdV) feline immunodeficiency virus (FIV) vectors produced using kits. Cell soluble purified (C3cs) was purchased commercially. Recombinant (C3rec) cDNA overexpressed Escherichia coli purified. The HTM...
To determine if proteasome inhibition using MG132 increased the efficiency of FIV vector-mediated transduction in human trabecular meshwork (TM)-1 cells and monkey organ-cultured anterior segments (MOCAS).TM-1 were pretreated for 1 hour with 0.5% dimethyl sulfoxide (DMSO; vehicle control) or 5 to 50 μM transduced FIV.GFP (green fluorescent protein)- FIV.mCherry-expressing vector at a multiplicity (MOT) 20. At 24 hours, fixed stained antibodies GFP, positive counted, manually by...
Current manufacturing of clinical grade lentiviral vectors (LVVs) for gene therapy applications commonly relies on transient transfection adherent 293T cells. Improvements in production efficiency and scalability would provide value meeting the needs increased amounts vector required development. An inducible producer cell line grown suspension culture represents a potentially more scalable process LVV which eliminates need costly plasmid reagents. We have engineered packaging by introducing...