A novel anaerobic bacterium that could ferment amino acids and organic was isolated from an anaerobic, propionate-oxidizing enrichment culture originating soil of a rice field in Japan. Cells the isolate, designated strain 4F6E(T), were Gram-staining-negative, oxidase- catalase-negative, non-spore-forming, vibrio-shaped, motile rods (0.8×2.0-2.5 µm) with two or three lateral flagella. Growth occurred at 20-42 °C (optimum 37-40 °C), pH 6.4-8.4 7.3) 0-1.5 % (w/v) NaCl 0-0.5 %). Good growth on...

An obligately anaerobic bacterial strain designated T-1-35 T was isolated as a dominant cultivable cellulose-degrading bacterium from soil of Japanese rice field an filter-paper degrader. Cells stained Gram-positive and were non-spore-forming rods with rounded ends, 0.8–1.0×3.5–15.0 µm, motile by means two to four polar flagella. exhibited pleomorphism: in aged cultures (over 90 days incubation), almost all cells irregularly shaped. Although no spore formation observed, tolerated high...

An obligately anaerobic bacterial strain (TW13 T ) affiliated with the family Clostridiaceae within class Clostridia was isolated from an anoxic soil sample subjected to reductive disinfestation. Cells of were stained Gram-positive and spore-forming straight rods a polar flagellum. The saccharolytic decomposed polysaccharides (glucomannan, pectin, starch xylan). produced acetate, butyrate H 2 as fermentation products carbohydrates utilized. major cellular fatty acids TW13 C 18:1 ω7c...

A pure culture of an obligately anaerobic, hydrogenotrophic, methanogenic archaeon, designated strain 169(T), which grows with hydrogen and carbon dioxide as the sole energy sources, was isolated from anaerobic propionate-oxidizing enrichment originally obtained inoculant rice-field soil in Japan. Cells 169(T) were non-motile, Gram-reaction-variable rod-shaped or slightly curved rods rounded ends (1.6-5.0 × 0.35-0.5 µm). Strain had fimbriae at both cell (up to ~10 per cell) but did not...

Neomacrophorin X (1) was isolated from Trichoderma sp. 1212-03. Heteronuclear multiple bond correlation (HMBC) spectral analysis indicated a unique [4.4.3]propellane framework, which verified by the 1H and 13C chemical shift calculations based on density functional theory (DFT) subsequent comparison with experimental data obtained in CDCl3. The DFT-based electronic circular dichroism (ECD) were effective not only determining absolute configuration but also confirming relative structure....

Rice fragrance is an important characteristic for Southeast Asian consumers, and fragrant landraces from Japan were first recorded in the 17th century. Principal component analysis clearly showed that Japanese genetically different non-Japanese landraces. composed of six clades, none which carried most common mutation, 8-bp deletion exon 7 Badh2. Fragrant comprised two major groups carrying Badh2 mutations. One group a known SNP at exon13 other exon1-intron1 junction as splicing donor site....

An obligately anaerobic bacterial strain designated KC3 T was isolated from a rice straw-degrading culture, for which soil of Japanese field used as the inoculum. Cells were determined to be non-cellulolytic, Gram-stain-positive, non-motile, ellipsoidal, spore-forming rods, 0.8–1×4–25 µm. Endospores formed at terminal position in elongated cells (12–25 µm, mean 15 µm). The temperature range growth 20–50 °C, with an optimum 37 °C. pH 5.0–7.5, 6.0 (slightly acidophilic). Strain fermented...

An aerobic bacterial strain designated AX-7T was isolated from the trunk surface of a Japanese beech (Fagus crenata). Cells were Gram-stain-negative, non-spore-forming, non-motile rods (1.0-1.2 µm in width and 1.2-3.0 length) with peritrichous fimbriae. capsulated, number them surrounded by thick slime layer. During growth, large aggregates formed, culture medium became viscous probably owing to exopolysaccharide release The temperature range for growth 10-37 °C, an optimum at 30 °C. pH...

An obligately anaerobic bacterial strain (CTTWT) belonging to the family Lachnospiraceae within class Clostridia was isolated from an anoxic soil sample subjected biological or reductive disinfestation. Cells of were Gram-stain-positive, short rods with peritrichous flagella. The saccharolytic and decomposed polysaccharides, chitin, xylan β-1,3-glucan. Strain CTTWT cell biomass cell-wall preparations ascomycete plant pathogen, Fusarium oxysporum f. sp. spinaciae. produced acetate, ethanol,...

In the budding yeast, Saccharomyces cerevisiae , protein kinases Ste20p (p21 Cdc42p/Rac -activated kinase), Ste11p [mitogen-activated kinase (MAPK) kinase], Ste7p (MAPK Fus3p, and Kss1p (MAPKs) are utilized for haploid mating, invasive growth, diploid filamentous growth. Members of highly conserved Ste20p/p65 PAK family regulate MAPK signal transduction pathways from yeast to man. We describe here a potent negative regulator in growth-signaling pathway. identified mutant, hsl7 that exhibits...

Abstract Characterization of methanotrophs isolated from paddy soils and rice (Oryza sativa) roots was investigated in the present study. The number root homogenates cultivar Mutsuhomare 4.9 × 107 most-probable-number (MPN) g−1 dry roots, Yumeakari it 2.0 108 MPN Kirara 4.6 roots. Although bacterial cells were observed infrequently on surface interiors before incubation, a large colonies, measuring 0.5–5 mm diameter, sterilized after incubation nitrate mineral agar plates under methane air....

A novel Gram-stain-positive rod-shaped actinobacterium was isolated from a soil conditioner made poultry manure. The isolate, designated strain MN-6-a(T), contained anteiso-C(15 : 0) and anteiso-C(17 as the major fatty acids, MK-7(H(2)) MK-8(H(2)) menaquinones. Phosphatidylglycerol polar lipid. G+C content of genomic DNA 67.4 mol%. Phylogenetic analysis showed that MN-6-a(T) closely related to Brevibacterium salitolerans TRM 415(T) with 97.1 % 16S rRNA gene sequence similarity. DNA-DNA...

A bacterial strain designated SK-11T was isolated from the acidic soil of a deciduous forest in Shirakami Mountains Japan. Cells were aerobic, non-motile, Gram-stain-negative rods, 0.7-1.0 µm width and 1.0-1.4 length. The pH range for growth between 4.0 5.5, with an optimum at 5.0. temperature 10 35 °C, around 25-30 °C. Strain utilized various carbohydrates as substrates well yeast extract protein hydrolysates. major cellular fatty acids (>10 % total acid contents) iso-C15 : 0 (55.4 %),...

An obligately anaerobic bacterial strain (TB5 T ) was isolated from a soil sample subjected to reductive or biological disinfestation. Cells of the were Gram-stain-positive, spore-forming and motile rods. The grew at 15–40 °C (optimum, 37 °C) pH 5.4–7.5 7.3). Strain TB5 utilized wide variety carbohydrates including polysaccharides (cellulose, xylan, starch, inulin, glucomannan laminarin) organic acids. Acetate, ethanol, H 2 CO products substrates utilized. major components cellular fatty...

Facultatively anaerobic bacterial strains were isolated from samples of a methanogenic reactor and, based on 16S rRNA gene sequences, found to be affiliated with the family Propionibacteriaceae in phylum Actinomycetota. Four almost-identical sequences comprehensively characterized. The most closely related species was Brooklawnia cerclae BL-34 T (96.4 % sequence similarity). Although phenotypic characteristics four identical, distinct differences some cellular and physiological properties...

A hydrogenotrophic motile methanogen was isolated from flooded Japanese paddy field soil. Anaerobic incubation of the soil on H(2)-CO(2) at 20 degrees C led to enrichment symmetrically curved autofluorescent rods. The methanogenic strain TM20-1 culture halotolerant and utilized H(2)-CO(2), 2-propanol-CO(2), or formate as a sole substrate. Based 16S rRNA gene sequence similarity (94.8%) with Methanospirillum hungateii, physiological phenotypic characteristics, suggested be newly identified...

An endo-acting family 3 pectate lyase (PL I) gene from Streptomyces thermocarboxydus was cloned and expressed in Escherichia coli. The deduced PL I sequence had highest similarity to a putative of S. coelicolor. Recombinant significantly lower specific activity, probably result incorrect folding, as indicated by circular dichroism spectra analysis.

A novel filamentous fungus strain designated RB-1 was isolated into pure culture from Japanese rice field soil through an anaerobic role tube technique. The is a mitosporic that grows in both aerobic and strict conditions using various mono-, di-, tri-, polysaccharides with acetate ethanol productions. amount of produced higher than cultures. characteristic verrucose or punctuate conidia closely resembled those some strains the genus Thermomyces , thermophilic mesophilic anamorphic...

A novel bacterial strain belonging to the genus Roseomonas was isolated from trunk surface of a mono maple (Acer mono) tree growing in Shirakami Mountains. The strain, designated R-1T, Gram-negative, non-motile, and oval-rod, formed reddish colonies on agar plates, as has previously been described for species. Although motility not observed, cells were peritrichously flagellated. Strain R-1T preferred organic acids over carbohydrates growth substrates. major cellular fatty acid C18:1 ω7c...