A5035951569- Plant Reproductive Biology
- Wheat and Barley Genetics and Pathology
- Genetic Mapping and Diversity in Plants and Animals
- Genetics and Plant Breeding
- Plant Virus Research Studies
- Plant and animal studies
- Genomics and Phylogenetic Studies
- Agricultural pest management studies
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Plant Molecular Biology Research
National Institute of Agricultural Botany
2020-2024
The University of Adelaide
2017-2019
Australian Centre for Plant Functional Genomics
2017
Abstract The current rate of yield gain in crops is insufficient to meet the predicted demands. Capturing boost from heterosis one few technologies that offers rapid gain. Hybrids are widely used for cereals, maize and rice, but it has been a challenge develop viable hybrid system bread wheat due genome complexity, which both large hexaploid. Wheat our most grown crop providing 20% calories humans. Here, we describe identification Ms1 , gene proposed use large-scale, low-cost production...
Summary Nuclear male‐sterile mutants with non‐conditional, recessive and strictly monogenic inheritance are useful for both hybrid conventional breeding systems, have long been a research focus many crops. In allohexaploid wheat, however, genic redundancy results in rarity of such mutants, the ethyl methanesulfonate‐induced mutant ms5 among few reported to date. Here, we identify TaMs5 as glycosylphosphatidylinositol‐anchored lipid transfer protein required normal pollen exine development,...
Bread wheat is strongly autogamous; however, an opportunity for outcrossing occurs when self-pollination fails and florets open. The first phase of floret opening at anthesis short induced by lodicule turgidity. Some re-open post-anthesis several days, known as the 'second opening', which underlying mechanisms are largely unknown. We performed detailed physiological, anatomical, histological investigations to understand biological basis flower process. Wheat were observed open ovary was...
In flowering plants, lipid biosynthesis and transport within anthers is essential for male reproductive success. TaMs1, a dominant wheat fertility gene located on chromosome 4BS, has been previously fine mapped identified to encode glycosylphosphatidylinositol (GPI)-anchored non-specific transfer protein (nsLTP). Although this critical pollen exine development, details of its function remains poorly understood. study, we report that TaMs1 only expressed from the B sub-genome, with highest...
Abstract Non-specific Lipid Transfer Proteins (nsLTPs) are involved in numerous biological processes. To date, only a fraction of wheat ( Triticum aestivum L.) nsLTPs (TaLTPs) have been identified, and even fewer functionally analysed. In this study, the identification, classification, phylogenetic reconstruction, chromosome distribution, functional annotation expression profiles TaLTPs were 461 putative identified from genome classified into five types (1, 2, C, D G). Phylogenetic analysis...
Cereal transformation and gene editing can be a complex costly undertaking. It is therefore important to validate understand the performance of components achieve high rates editing. Here, we have made direct comparison different CRISPR/Cas9 guide systems target genome in three cereal species. We show that sequences driven by same pol II promoter rice, wheat barley large differences efficiency. The seen were based on way guides presented factors outside sequence itself. While both tRNA...