A5033088858- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Plant Parasitism and Resistance
- Plant pathogens and resistance mechanisms
- Coffee research and impacts
- Plant Virus Research Studies
University of Toronto
2017-2023
A plant pan-genome immunity landscape Plant pathogens elicit an immune response through effector proteins. In turn, genomes encode genes that determine species-specific recognition of these effectors by a process known collectively as effector-triggered (ETI). By examining range strains the pathogen Pseudomonas syringae infect model Arabidopsis thaliana , Laflamme et al. generated P. Type III Effector Compendium (PsyTEC) and in turn identified responsible for ETI Arabidopsis. This analysis...
Diverse Gram-negative pathogens like Pseudomonas syringae employ type III secreted effector (T3SE) proteins as primary virulence factors that combat host immunity and promote disease. T3SEs can also be recognized by plant hosts activate an triggered immune (ETI) response shifts the interaction back toward immunity. Consequently, are pivotal in determining potential of individual P. strains, ultimately help to restrict a subset unable recognize their repertoires T3SEs. While number families...
Pseudomonas syringae is a highly diverse bacterial species complex capable of causing wide range serious diseases on numerous agronomically important crops. We examine the evolutionary relationships 391 agricultural and environmental strains using whole-genome sequencing genomic analyses.We describe phylogenetic distribution all 77,728 orthologous gene families in pan-genome, reconstruct core genome phylogeny 2410 genes, hierarchically cluster accessory genome, identify diversity type III...
The Arabidopsis nucleotide-binding leucine-rich repeat protein ZAR1 can recognize at least six distinct families of pathogenic effector proteins to mount an effector-triggered immune response. This remarkable immunodiversity appears be conveyed by receptor-like cytoplasmic kinase (RLCK) complexes, which associate with sense several effector-induced perturbations. Here we show that the recently identified ZAR1-mediated responses against HopX1, HopO1, and HopBA1 Pseudomonas syringae rely on...
Pseudomonas syringae is a genetically diverse bacterial species complex responsible for numerous agronomically important crop diseases. Individual P . isolates are assigned pathovar designations based on their host of isolation and the associated disease symptoms, these often assumed to reflect specificity although this assumption has rarely been rigorously tested. Here we developed rapid seed infection assay measure virulence 121 common bean ( Phaseolus vulgaris ). This collection includes...
ABSTRACT Pseudomonas syringae is a highly diverse bacterial species complex capable of causing wide range serious diseases on numerous agronomically important crop species. Here, we examine the evolutionary relationships 391 agricultural and environmental strains from P. using whole-genome sequencing genomic analyses. Our collection includes 11 13 previously described phylogroups isolated off over 90 hosts. We describe phylogenetic distribution all orthologous gene families in pan-genome,...
Abstract Foliar spots caused by Pseudomonas coronafaciens pv. garcae (Pcg), amygdali tabaci (Pat) and cichorii (Pch) are major bacterial diseases that can reduce coffee production. However, little is known about the genetic diversity molecular mechanisms underlying pathogenicity to plants of these bacteria. In this study, genome sequences Pcg, Pat Pch strains isolated from in Minas Gerais state, Brazil, were used assess their variability plasticity, compare type III secretion system (T3SS)...
Summary The emergence of new pathogens is an ongoing threat to human health and agriculture. While zoonotic spillovers received considerable attention, the crop diseases less well studied. Here, we identify genomic factors associated with Pseudomonas syringae bacterial blight coffee. Fifty‐three P. strains from diseased Brazilian coffee plants were sequenced. Comparative evolutionary analyses used loci blight. Growth symptomology assays performed validate findings. Coffee isolates clustered...
ABSTRACT Diverse Gram-negative pathogens like Pseudomonas syringae employ type III secreted effector (T3SE) proteins as primary virulence factors that combat host immunity and promote disease. T3SEs can also be recognized by plant hosts activate an triggered immune (ETI) response shifts the interaction back towards immunity. Consequently, are pivotal in determining potential of individual P. strains, ultimately restrict to a subset unable recognize their repertoires T3SEs. While number...
ABSTRACT Pseudomonas syringae is a genetically diverse bacterial species complex responsible for numerous agronomically important crop diseases. Individual P. isolates are typically given pathovar designations based on their host of isolation and the associated disease symptoms, these often assumed to reflect specificity although this assumption has rarely been rigorously tested. Here we developed rapid seed infection assay measure virulence 121 common bean ( Phaseolus vulgaris ). This...