Thousand-and-one amino acid kinases (TAOK) 1 and 2 are activated catalytically during mitosis can contribute to mitotic cell rounding spindle positioning. Here, we characterize a compound that inhibits TAOK1 TAOK2 activity with IC50 values of 11 15 nmol/L, is ATP-competitive, targets these selectively. TAOK inhibition or depletion in centrosome-amplified SKBR3 BT549 breast cancer models increases the population, percentages cells displaying amplified centrosomes multipolar spindles, induces...

The teneurins, also known as Ten-m/Odz, are highly conserved type II transmembrane glycoproteins widely expressed throughout the nervous system. Functioning dimers, these large cell-surface adhesion proteins play a key role in regulating neurodevelopmental processes such axon targeting, synaptogenesis and neuronal wiring. Synaptic specificity is driven by molecular interactions, which can occur either trans-homophilic manner between teneurins or through trans-heterophilic interaction across...

Hypoxic-ischemic (HI) encephalopathy is a cerebrovascular injury caused by oxygen deprivation to the brain and remains major cause of neonatal mortality morbidity worldwide. Therapeutic hypothermia current standard care but it does not provide complete neuroprotection. Our aim was investigate neuroprotective effect oleuropein (Ole) in (seven-day-old) mouse model HI. Ole, secoiridoid found olive leaves, has previously shown reduce damage against cerebral other ischemia/reperfusion injuries....

Class II antiarrhythmics or <i>β</i>-blockers are antisympathetic nervous system agents that act by blocking <i>β</i>-adrenoceptors. Despite their common clinical use, little is known about the effects of on free intracellular calcium (Ca²⁺_i), an important cytosolic second messenger and a key regulator cell function. We investigated role four chemical analogs, commonly prescribed (atenolol, metoprolol, propranolol, sotalol), Ca²⁺_i release...

ABSTRACT Neural tube defects (NTDs) are the second most common cause of congenital malformations and often studied in animal models. Loop-tail (Lp) mice carry a mutation Vangl2 gene, member Wnt-planar cell polarity pathway. In Vangl2+/Lp embryos, induces failure completion caudal neural closure, but only small percentage embryos develop open spina bifida. Here, we show that majority developed closed NTDs presented cellular aggregates may facilitate sealing these defects. The expressed crest...

The FEBS‐IUBMB‐ENABLE 1st International Molecular Biosciences PhD and Postdoc Conference was held in Seville, Spain, from the 16–18th of November 2022. Nearly 300 participants all over globe were welcomed by host institution, Institute Biomedicine Seville (IBiS). Following theme “The perfect tandem: How technology expands frontiers biomedicine”, Scientific Symposium conference hosted eight world‐renowned keynote speakers who presented their work one four sessions: Innovation , Basic Research...

&lt;p&gt;Live images of growing MCF-10A cells. Representative time-lapse video showing GFP-α-tubulin expressing MCF10-A cells incubated in growth medium containing equivalent DMSO (0.05%) for 48 hours.&lt;/p&gt;

&lt;p&gt;Supplementary Materials, Methods and Legends&lt;/p&gt;

&lt;p&gt;Supplementary Figure S1. A-B, Compounds 43 and 63 are ATP-competitive inhibitors of TAOK1 TAOK2 activity. A, compound or B, (0.0001-1 µM) were incubated with three ATP concentrations (1 µM, 15 µM 155 in vitro kinase assays using MBP as substrate. Calculated IC50 values presented for each TAOK when the presence either raised by increasing concentration (shown µM); n = 3. C, Compound does not inhibit phosphorylation activation JNK TAOKs cells. COS1 cells cotransfected FLAG-JNK...

&lt;p&gt;Live images showing compound 43 delays mitosis and induces mitotic catastrophe in CA SKBR3 cells. Representative time-lapse video GFP-α-tubulin expressing cells incubated growth medium containing (5 µM) for 48 hours.&lt;/p&gt;

&lt;p&gt;Live images of growing SKBR3 cells. Representative time-lapse video showing GFP-α-tubulin expressing cells incubated in growth medium containing equivalent DMSO (0.05%) for 48 hours.&lt;/p&gt;

&lt;p&gt;Live images of growing MCF-10A cells treated with compound 43. Representative time-lapse video showing GFP-α-tubulin expressing MCF10-A incubated in growth medium containing 43 (5 µM) for 48 hours.&lt;/p&gt;

&lt;div&gt;Abstract&lt;p&gt;Thousand-and-one amino acid kinases (TAOK) 1 and 2 are activated catalytically during mitosis can contribute to mitotic cell rounding spindle positioning. Here, we characterize a compound that inhibits TAOK1 TAOK2 activity with IC&lt;sub&gt;50&lt;/sub&gt; values of 11 15 nmol/L, is ATP-competitive, targets these selectively. TAOK inhibition or depletion in centrosome-amplified SKBR3 BT549 breast cancer models increases the population, percentages cells displaying...

&lt;div&gt;Abstract&lt;p&gt;Thousand-and-one amino acid kinases (TAOK) 1 and 2 are activated catalytically during mitosis can contribute to mitotic cell rounding spindle positioning. Here, we characterize a compound that inhibits TAOK1 TAOK2 activity with IC&lt;sub&gt;50&lt;/sub&gt; values of 11 15 nmol/L, is ATP-competitive, targets these selectively. TAOK inhibition or depletion in centrosome-amplified SKBR3 BT549 breast cancer models increases the population, percentages cells displaying...

&lt;p&gt;Live images of growing SKBR3 cells. Representative time-lapse video showing GFP-α-tubulin expressing cells incubated in growth medium containing equivalent DMSO (0.05%) for 48 hours.&lt;/p&gt;

&lt;p&gt;Live images of growing MCF-10A cells. Representative time-lapse video showing GFP-α-tubulin expressing MCF10-A cells incubated in growth medium containing equivalent DMSO (0.05%) for 48 hours.&lt;/p&gt;

&lt;p&gt;Live images of growing MCF-10A cells treated with compound 43. Representative time-lapse video showing GFP-α-tubulin expressing MCF10-A incubated in growth medium containing 43 (5 µM) for 48 hours.&lt;/p&gt;

&lt;p&gt;Supplementary Figure S1. A-B, Compounds 43 and 63 are ATP-competitive inhibitors of TAOK1 TAOK2 activity. A, compound or B, (0.0001-1 µM) were incubated with three ATP concentrations (1 µM, 15 µM 155 in vitro kinase assays using MBP as substrate. Calculated IC50 values presented for each TAOK when the presence either raised by increasing concentration (shown µM); n = 3. C, Compound does not inhibit phosphorylation activation JNK TAOKs cells. COS1 cells cotransfected FLAG-JNK...

&lt;p&gt;Supplementary Materials, Methods and Legends&lt;/p&gt;

&lt;p&gt;Live images showing compound 43 delays mitosis and induces mitotic catastrophe in CA SKBR3 cells. Representative time-lapse video GFP-α-tubulin expressing cells incubated growth medium containing (5 µM) for 48 hours.&lt;/p&gt;

Adequate nutrient supply is crucial for the proper development of embryo. Although determined by maternal diet, gut microbiota also influences availability. While currently there no cure neural tube defects (NTDs), their prevention largely amenable to folic acid and inositol supplementation. The contributes production these nutrients, which are absorbed host, but its role in this context remains unexplored. In study, we performed a functional morphological analysis intestinal tract loop-tail...