A5036154202- Retinal Development and Disorders
- Zebrafish Biomedical Research Applications
- Photoreceptor and optogenetics research
- Neural dynamics and brain function
- Visual perception and processing mechanisms
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Receptor Mechanisms and Signaling
- Neurobiology and Insect Physiology Research
- Fish biology, ecology, and behavior
- Spaceflight effects on biology
- Physiological and biochemical adaptations
- Vestibular and auditory disorders
- Neuroscience and Neuropharmacology Research
- Advanced Fluorescence Microscopy Techniques
- Memory and Neural Mechanisms
- Zoonotic diseases and public health
- Neuroinflammation and Neurodegeneration Mechanisms
- Academic Publishing and Open Access
- Single-cell and spatial transcriptomics
- Circadian rhythm and melatonin
University College London
2019-2024
King's College London
2013-2019
Hundreds of human genes are associated with neurological diseases, but translation into tractable biological mechanisms is lagging. Larval zebrafish an attractive model to investigate genetic contributions diseases. However, current CRISPR-Cas9 methods difficult apply large screens studying behavioural phenotypes. To facilitate rapid screening, we developed a simple sequencing-free tool validate gRNAs and highly effective method capable converting >90% injected embryos directly F0...
Abstract The larval zebrafish ( Danio rerio ) is an excellent vertebrate model for in vivo imaging of biological phenomena at subcellular, cellular and systems levels. However, the optical accessibility highly pigmented tissues, like eyes, limited even this animal model. Typical strategies to improve transparency larvae require use either toxic chemical compounds (e.g. 1-phenyl-2-thiourea, PTU) or pigmentation mutant strains casper mutant). To date none these produce normally behaving that...
A striking feature of the CNS is precise wiring its neuronal connections. During vertebrate visual system development, different subtypes retinal ganglion cells (RGCs) form specific connections with their corresponding synaptic partners. However, underlying molecular mechanisms remain to be fully elucidated. Here, we report that cell-adhesive transmembrane protein Teneurin-3 (Tenm3) required by zebrafish RGCs for acquisition correct morphological and functional connectivity in vivo....
For many species, hunting is an innate behaviour that crucial for survival, yet the circuits control predatory action sequences are poorly understood. We used larval zebrafish to identify a population of pretectal neurons hunting. By combining calcium imaging with virtual assay, we identified discrete region selectively active when animals initiate Targeted genetic labelling allowed us examine function and morphology individual cells two classes neuron project ipsilateral optic tectum or...
Optogenetic actuators with diverse spectral tuning, ion selectivity and kinetics are constantly being engineered providing powerful tools for controlling neural activity subcellular resolution millisecond precision. Achieving reliable interpretable in vivo optogenetic manipulations requires reproducible actuator expression calibration of photocurrents target neurons. Here, we developed nine transgenic zebrafish lines stable opsin calibrated their efficacy vivo. We first used high-throughput...
The orientation of visual stimuli is a salient feature scenes. In vertebrates, the first neural processing steps generating selectivity take place in retina. Here, we dissect an orientation-selective circuit larval zebrafish retina and describe its underlying synaptic, cellular, molecular mechanisms. We genetically identify class amacrine cells (ACs) with elongated dendritic arbors that show tuning. Both selective optogenetic ablation ACs marked by cell-adhesion molecule Teneurin-3 (Tenm3)...
The teneurins, also known as Ten-m/Odz, are highly conserved type II transmembrane glycoproteins widely expressed throughout the nervous system. Functioning dimers, these large cell-surface adhesion proteins play a key role in regulating neurodevelopmental processes such axon targeting, synaptogenesis and neuronal wiring. Synaptic specificity is driven by molecular interactions, which can occur either trans-homophilic manner between teneurins or through trans-heterophilic interaction across...
ABSTRACT Hundreds of human genes are associated with neurological diseases, but translation into tractable biological mechanisms is lagging. Larval zebrafish an attractive model to investigate genetic contributions diseases. However, current CRISPR-Cas9 methods difficult apply large screens studying behavioural phenotypes. To facilitate rapid screening, we developed a simple sequencing-free tool validate gRNAs and highly effective method capable converting >90% injected embryos directly...
Abstract For many species, hunting is an innate behaviour that crucial for survival, yet the circuits control predatory action sequences are poorly understood. We used larval zebrafish to identify a command system controls hunting. By combining calcium imaging with virtual assay, we identified discrete pretectal region selectively active when animals initiate Targeted genetic labelling allowed us examine function and morphology of individual cells two classes neuron project ipsilateral optic...
Abstract Optogenetic actuators with diverse spectral tuning, ion selectivity and kinetics are constantly being engineered providing powerful tools for controlling neural activity subcellular resolution millisecond precision. Achieving reliable interpretable in vivo optogenetic manipulations requires reproducible actuator expression calibration of photocurrents target neurons. Here, we developed nine transgenic zebrafish lines stable opsin calibrated their efficacy . We first used...