A5067497300- Viral Infectious Diseases and Gene Expression in Insects
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Fluid Dynamics and Mixing
- Protein purification and stability
- Skin and Cellular Biology Research
- Advanced Chemical Sensor Technologies
- Enzyme Production and Characterization
- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Extraction and Separation Processes
- thermodynamics and calorimetric analyses
- Meat and Animal Product Quality
- Microbial Inactivation Methods
- SARS-CoV-2 and COVID-19 Research
- Protein Structure and Dynamics
- Membrane Separation and Gas Transport
- Minerals Flotation and Separation Techniques
- Silk-based biomaterials and applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Computational Drug Discovery Methods
- Virus-based gene therapy research
- Evolution and Genetic Dynamics
- Enzyme Catalysis and Immobilization
- Microtubule and mitosis dynamics
Harvard University Press
2024-2025
Harvard University
2019-2024
Zhejiang Sci-Tech University
2024
Zero to Three
2023
University of Illinois Urbana-Champaign
2019-2020
Massachusetts Institute of Technology
1987-2009
Abstract A procedure for measuring the rate of heat production from a fermentation has been developed. The method is based on temperature rise broth resulting metabolism, when controller turned off. accumulation measured in this manner then corrected losses and gains. sensitive thermistor used to follow with time. This shown be as accurate previous methods but much simpler execution. Using technique, during metabolism was found correlate oxygen consumption. Experiments were performed using...
One of the main problems in culture Chinese Hamster Ovary (CHO) cells continues to be inability maintain viability cultures over an extended period time. The rapid decline at end is exacerbated by absence serum. In trying reduce extent death these cultures, we first tried determine mode death. We found that more than 80% a standard serum-free batch CHO suspension died via apoptosis—as evidenced condensed chromatin and appearance characteristic DNA ladder. Furthermore, when protein synthesis...
Abstract The method of using liquid emulsion membranes featuring the cation carrier D2EHPA [di‐(2‐ethylhexyl) phosphoric acid] for separation L ‐phenylalanine is examined. Results from experiments performed under various conditions are discussed and an optimal condition determined. selectivity membrane system discussed. effects impurities such as sodium chloride, glucose, lactic acid, ‐tryptophan on transport evaluated. It shown that a potential operation not only to separate but also...
Abstract The separation and concentration of amino acids using liquid emulsion membranes (LEMs) are discussed. Using L ‐ phenylalanines as a model solute, it is experimentally shown facilitated transport system that can be simultaneously achieved. rate separation, final product concentration, membrane swell to increase with increasing chloride driving forces in the membrane, These effects insensitive particular salt used force. Changes carrier result higher initial fluxes rates....
Abstract This study reports some findings on the death of mammalian cells occurring from fluid dynamic effects when they are pumped through capillaries. The cell strains used were human HeLa S3 and mouse L929, grown in monolayer culture. Cells harvested suspensions various lengths diameters stainless steel capillary tubing. Viability was assayed by dye exclusion test. Cell deaths occurred could be correlated with either average wall shear or power dissipation within tube. L929 found to more...
Abstract In most enzymatic reactions, batch or continuous, separation of the enzyme for reuse is difficult if not impossible. A process will be presented in which an Ultrafiltration membrane serves to separate reaction products from and substrate. this manner may retained re‐used. Furthermore, under these conditions, need only present catalytic amounts regardless amount product produced. Under proper operating conditions ultrafiltration selection, a pure solution α‐amylase Bacillus subtilis...
SARS-CoV-2 employs its spike protein’s receptor binding domain (RBD) to enter host cells. The RBD is constantly subjected immune responses, while requiring efficient cell receptors for successful infection. However, our understanding of how RBD’s biophysical properties contribute SARS-CoV-2’s epidemiological fitness remains largely incomplete. Through a comprehensive approach, comprising large-scale sequence analysis variants and the identification function based on thermodynamics, we...
Abstract For the cultivation of mammalian cells on microcarriers a minimum inoculum concentration is required to initiate cell attachment and subsequent growth. A critical number model has been proposed elucidate mechanism requirement. In this it was hypothesized that after inoculation per microcarrier for normal growth occur; failure acquire enough will impede This expressed mathematically used simulate distribution under different conditions. By comparing simulated kinetics with...
The major objective of this project is to achieve the direct microbiological conversion cellulosic biomass liquid fuel, ethanol. Within scope objective, it also intent maximize efficiency ethanol production from biomass. This can be achieved through effective utilization both (six-carbon sugar) and hemicellulosic (five-carbon fractions in degradation use a thermophilic anaerobic bacterium, Clostridium thermocellum . microorganism unique that able hydrolyse but, unfortunately, not metabolize...
The early detection of high-fitness viral variants is critical for pandemic response, yet limited experimental resources at the onset variant emergence hinder effective identification. To address this, we introduce an active learning framework that integrates protein language model ESM3, Gaussian process with uncertainty estimation, and a bio-physical to predict fitness novel in few-shot setting. By benchmarking on past SARS-CoV-2 data, demonstrate our methods accelerates identification by...
Abstract The use of immobilization in extractive lactic acid fermentation by Lactobacillus delbrueckii is preferred. In this article, the mathematical simulations to examine influences substrate and product transport were performed assess overall performance. showed that k ‐carrageenan beads was not a rate limiting factor. However, model observed significant buildup inhibitory large beads. validated through comparisons with experimental results. Finally, used predict performance under...
Abstract The performance of a penicillin fermentation was assessed in laboratory‐scale bubble column fermentor, with mycelial growth confined to the pore matrix celite beads. Final cell densities 29 g/L and titres 5.5 were obtained cultures. In comparison, cultures free cells grown absence beads experienced dissolved oxygen limitations column, giving only 17 final concentrations equally low 2 g/L. better attributed enhanced gas liquid mass transfer rates, coefficients ( k L ) two three times...
Abstract A variety of diploid human fibroblast lines have been successfully grown to high densities (>10 6 cell/ml) on recently developed microcarriers. Interferon induction using poly I·poly C and a superinduction procedure resulted in yields greater than 10,000 units/ml with one cell line. direct comparison microcarrier cultures roller bottle showed equivalent interferon per basis some apparent differences relating optimum inducer concentrations kinetics accumulation.
Abstract A high molecular weight intracellular enzyme of Bacillus brevis ATCC 9999 is released when the organism disrupted by sonication homogenization. However, both processes also degrade enzyme. Assays for protein release and specific enzymatic activity indicate that degradation can be represented first‐order kinetic models. Utilization difference between kinetics allows optimization in recovery this homogenization processes.
We investigate the rheological properties of interpenetrating networks reconstituted from main cytoskeletal components: filamentous actin, microtubules, and vimentin intermediate filaments. The elastic modulus is determined largely by with little contribution either microtubules or vimentin. However, dramatically impacts relaxation, even small amounts significantly increasing relaxation time network. This highly unusual decoupling between dissipation elasticity may reflect weak attractive...
Recent studies have shown that dielectric properties of raw potato can be predicted over the range 300–3000 Mhz and 5–65°C by a noninteractive Distributive model derived from lumped circuit analysis two-phase approximation which treats as binary system consisting an inert solid phase active liquid phase. Dielectric behavior was seen to result primarily water ion activities aqueous regions but subject appreciable modification mechanism volume exclusion due effects colloidal solids. Cell-free...
Recombinant Chinese hamster ovary (rCHO) cells were cultivated on microcarriers for the production of human immune (Gamma) interferon. The effect basal medium, serum, and microcarrier concentration interferon was investigated. specific productivity in post-confluent stage similar to that growth stage. Control pH results a significant improvement volumetric production. rate by these rCHO did not decrease after one month cultivation microcarriers.