A5070885316- Dental materials and restorations
- Dental Implant Techniques and Outcomes
- Oral microbiology and periodontitis research
- Antifungal resistance and susceptibility
- Bone Tissue Engineering Materials
- Bacterial biofilms and quorum sensing
- Endodontics and Root Canal Treatments
- Dental Radiography and Imaging
- Dental Erosion and Treatment
- Dental Health and Care Utilization
- Orthopaedic implants and arthroplasty
- Temporomandibular Joint Disorders
- Dental Research and COVID-19
- Fungal Infections and Studies
- Salivary Gland Disorders and Functions
- Business and Management Studies
- Fluoride Effects and Removal
- Infective Endocarditis Diagnosis and Management
- Laser Applications in Dentistry and Medicine
- Oral and Maxillofacial Pathology
- Antimicrobial agents and applications
- Oropharyngeal Anatomy and Pathologies
- Bone and Dental Protein Studies
- Probiotics and Fermented Foods
- Antimicrobial Peptides and Activities
Universidade Estadual de Campinas (UNICAMP)
2015-2024
KU Leuven
2015-2020
Altair Engineering (United States)
2016
Hospital de Clínicas da Unicamp
2015
Forest Science and Research Institute
2014
Prince Philip Dental Hospital
2008-2013
University of Hong Kong
2008-2013
Pontifícia Universidade Católica do Paraná
2013
Methodist University of Piracicaba
2007-2010
British Society of Periodontology
2006
2, 3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT) reduction assay has been used to study Candida biofilm formation. However, considering that the XTT is dependent on cell activity, its use for evaluating mature biofilms may lead inaccuracies since bottom layers tend be relatively quiescent at later stages of The aim this was improve by adding glucose supplements standard formulation. albicans ATCC 90028 form 24-, 48- and 72-h biofilms....
Objectives To evaluate the effect of sodium hypochlorite ( N a OC l) on biofilms, colour stability (Δ E ) and surface roughness R a) complete dentures patient acceptability. Background Denture cleansers should be able to reduce accumulation biofilms without affecting acrylic resin properties. Patient satisfaction is important maintaining their daily use. Materials methods Fifteen participants were instructed keep immersed in 0.5% l solution for 3 min over 90 days. Swabs taken from inoculated...
This study examined the influence of bacteria on virulence and pathogenicity candidal biofilms. Mature biofilms (Candida albicans-only, bacteria-only, C. albicans with bacteria) were generated acrylic either analysed directly, or used to infect a reconstituted human oral epithelium (RHOE). Analyses included Candida hyphae enumeration assessment gene expression. Lactate dehydrogenase (LDH) activity tissue invasion following biofilm infection RHOE also measured. more prevalent (p < 0.05) in...
Biofilm-associated implant infections represent a serious public health problem. Covalent immobilization of antimicrobial agents on titanium (Ti), thereby inhibiting biofilm formation microbial pathogens, is solution to this Vancomycin (VAN) and caspofungin (CAS) were covalently bound Ti substrates using an improved processing technique adapted large-scale coating implants. Resistance the VAN-coated (VAN-Ti) CAS-coated (CAS-Ti) against in vitro bacterium Staphylococcus aureus fungal pathogen...
Preload loss can favor the occurrence of implant-abutment interface misfit, and bacterial colonization at this may lead to implant failure. The aim study was evaluate preload penetration through conical external hexagon connection systems subjected thermal cycling mechanical fatigue (TM). Four different were evaluated (n=6): with universal post, Morse taper post bolt, locking standard abutment. assemblies (implant-abutment) a regimen (1,000 cycles 5°C 55°C) (1.0 million cycles, 1.0 Hz, 120...
The antimicrobial action of five drugs incorporated in temporary denture relines on the fungal biofilm was evaluated.A Candida albicans (SC5314) formed specimens (10 × 1 mm) materials (Trusoft and Softone) modified or not (control) by (nystatin, miconazole, ketoconazole, itraconazole, chlorhexidine diacetate). Cell viability determined spectrophotometrically tetrazolium salt reduction assay (XTT) after 24 h, 48 7 14 days incubation. minimum inhibitory concentrations (MICs) were those which...
AbstractWhilst Candida albicans occurs in peri-implant biofilms, its role peri-implantitis remains unclear. This study therefore examined the virulence of C. mixed-species biofilms on titanium surfaces. Biofilms (Ca), with streptococci (Streptococcus sanguinis, S. mutans) (Ca-Ss-Sm) and those incorporating Porphyromonas gingivalis (Ca-Pg Ca-Ss-Sm-Pg) were developed. Expression genes associated adhesion (ALS1, ALS3, HWP1) hydrolytic enzymes (SAP2, SAP4, SAP6, PLD1) was measured hyphal...
This study evaluated the long-term efficacy of denture cleansers against Candida spp. biofilm recolonization on liner surface. Specimens were fabricated a poly(methyl methacrylate)-based and had their surface roughness at baseline after cleansing treatments. C. albicans or glabrata biofilms formed for 48 h, then specimens randomly assigned to one cleaning treatments: two alkaline peroxides (soaking 3 15 min), 0.5% sodium hypochlorite (10 min) distilled water (control; min). After treatments,...
The aim of this study was to evaluate the correlation between patients' and dentist's assessment dentures correlate these variables with objective measures masticatory function. A sample 28 edentulous individuals selected, all wearing both complete for at least 6 months no signs or symptoms temporomandibular joint disorders. They rated their level satisfaction from 0 100 by means a visual analogue scale, were scored dentist 9 considering functional aspects. Tooth wear evaluated on posterior...
Although the effect of acidulated phosphate fluoride gel (APF gel) on caries reduction in permanent teeth is based evidence, relevance clinical application time still under debate. Also, 4- versus 1-min has not been evaluated deciduous enamel. In a blind, crossover, situ study 14 days, 16 adult volunteers wore palatal appliances containing slabs human and At beginning each phase, were submitted to one following treatments: no APF (negative control); (1.23% F) for 1 or 4 min. Biofilm...
Abstract The aim of this study was to evaluate the bioactivity and architecture Candida albicans biofilms developed on surface poly(methyl methacrylate) (PMMA) resin. To do this, roughness (SR) free energy PMMA specimens were measured. Next, two different C. strains (ATCC 90028 SC5314) allowed develop evaluated at 24, 48, 72 h after adhesion. measured by XTT reduction assay. Biofilm topography scanning electron microscopy. Confocal microscopy used architectural properties bio‐volume, average...
It is well known that the use of denture cleansers can reduce Candida albicans biofilm accumulation; however, efficacy citric acid uncertain. In addition, long-term this cleanser not established, and their effect on residual biofilms unknown. This in vitro study evaluated treatment C. recolonization poly(methyl methacrylate) (PMMA) surface. were developed for 72 h PMMA resin specimens (n = 168), which randomly assigned to 1 3 cleansing treatments (CTs) overnight (8 h). CTs included purified...
The purpose of this study was to investigate whether dietary carbohydrates can modulate the development Candida albicans biofilms on denture material surface. Poly (methyl methacrylate) acrylic resin discs were fabricated and had their surface roughness measured. Biofilms C. ATCC 90028 developed saliva-coated specimens in culture medium without (control) or with carbohydrate supplementation by starch, starch+sucrose, glucose, sucrose for 72 h. cell count, metabolic activity, biovolume,...
The aim of this study was to evaluate the influence implant macrodesign when using different types collar and thread designs on stress/strain distributions in a maxillary bone site.Six groups were obtained from combination two (smooth microthread) three shapes (square, trapezoidal, triangular) external hexagon implants (4 × 10 mm) supporting single zirconia crown first molar region. A 200-N axial occlusal load applied crown, measurements made von Mises stress (σvM) for implant, tensile...
Monolithic zirconia crowns have many favorable properties and may potentially be used to solve dental problems such as chipping. However, monolithic crown resistance can affected by its phase transformation when subjected low temperatures, humidity, stress. This study evaluated the fracture load of submitted different thermal mechanical aging tests. Seventy were randomly divided into following five groups: control, no treatment; hydrothermal at 122°C, two bar for one hour; fatigue, 104...
Denture liners (DL) are easily colonized by Candida spp. In an attempt to prevent biofilm colonization, manufacturers have incorporated undecylenic acid (UDA) into DL. this in vitro study, the effects of UDA released from DL on biofilms were investigated. The concentrations commercial determined gas chromatography–mass spectrometry (GC-MS). Minimum inhibitory concentration (MIC) and minimum fungistatic (MFC) tests performed for C. albicans or glabrata, with comparison Specimens (experimental...