A5028609525- Oral microbiology and periodontitis research
- Antifungal resistance and susceptibility
- Infective Endocarditis Diagnosis and Management
- Bacterial biofilms and quorum sensing
- Streptococcal Infections and Treatments
- Otolaryngology and Infectious Diseases
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Microbial Metabolism and Applications
- Probiotics and Fermented Foods
- HIV/AIDS oral health manifestations
- Antimicrobial Resistance in Staphylococcus
- Fungal Infections and Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Respiratory and Cough-Related Research
- Food composition and properties
- Biofuel production and bioconversion
- RNA and protein synthesis mechanisms
- Bee Products Chemical Analysis
- Hemoglobinopathies and Related Disorders
- Renal Diseases and Glomerulopathies
- Bacterial Genetics and Biotechnology
- Advanced Drug Delivery Systems
- Steroid Chemistry and Biochemistry
- Bacterial Identification and Susceptibility Testing
Curtin University
2021-2024
University of Hong Kong
2006-2016
Chinese University of Hong Kong
2009-2016
University of Peradeniya
2009-2015
The University of Western Australia
2015
Prince Philip Dental Hospital
2009
Bio-Medical Science (South Korea)
2009
British Society of Periodontology
2009
Enterococci are considered as transient constituent components of the oral microbiome that may cause a variety and systemic infections. As there is sparse data on enterococcal prevalence, we evaluated Enterococcus spp. their virulence attributes including antimicrobial resistance in healthy Brazilian cohort. A total 240 individuals different age groups were studied (children 4-11 yrs, adolescents 12-17 young adults 18-29 30-59 elderly over 60 yrs). Oral rinses collected isolates identified...
2, 3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT) reduction assay has been used to study Candida biofilm formation. However, considering that the XTT is dependent on cell activity, its use for evaluating mature biofilms may lead inaccuracies since bottom layers tend be relatively quiescent at later stages of The aim this was improve by adding glucose supplements standard formulation. albicans ATCC 90028 form 24-, 48- and 72-h biofilms....
An in vitro assay to study multiple Candida biofilms, parallel, has been carried out using the Calgary biofilm device (CBD). We here report: i) standardization of CBD for albicans formation, ii) kinetics C. iii) formation by five species, and iv) effect dietary carbohydrates on formation. The metabolic activity all pegs was similar (p=0.6693) revealed slow growth up 36 h significantly higher 48 (p<0.001). Significant differences total were seen glucose, fructose lactose grown compared...
The familial clustering observed in chronic kidney disease of uncertain etiology (CKDu) characterized by tubulointerstitial damages the North Central Region Sri Lanka strongly suggests involvement genetic factors its pathogenesis. objective present study is to use whole-exome sequencing identify variants associated with CKDu. Whole-exome eight CKDu cases and controls was performed, followed direct candidate loci 301 276 controls. Association revealed rs34970857 (c.658G > A/p.V220M) located...
Biofilms are aggregates of microorganisms living in multilayered structures inside polymeric matrices onto surfaces. These biofilms may subvert the physiological properties adjacent tissues causing morphofunctional failure. Many studies have shown that expression virulence attributes is maximized when microbes form such communities. This study evaluated differential phospholipasic activity Candida albicans SC5314 grown planktonic phase and biofilm. We propose two distinct protocols for...
This study aimed to evaluate the effects of fluconazole or nystatin exposure on developed Candida albicans biofilms regarding their exopolysaccharide matrix. The minimal inhibitory concentration (MIC) against was determined for C. reference strain (ATCC 90028). Poly(methlymethacrylate) resin (PMMA) specimens were fabricated according manufacturer's instructions and had surface roughness measured. Biofilms surfaces 48 h after that exposed during 24 prepared in a medium at MIC, 10 x MIC 100...
Official journal of the Postgraduate Institute Agriculture, University Peradeniya. Tropical Agricultural Research publishes complete accounts research outstanding significance, together with critical review articles on all aspects agriculture including agronomy and crop production, horticulture, agricultural biology, food nutrition, soil water resource management, engineering, economics, animal science, biotechnology, plant protection, genetics breeding.Tropical is now indexed in DOAJ.
Abstract In Staphylococcus aureus, most multiresistance plasmids lack conjugation or mobilization genes for horizontal transfer. However, are mobilizable due to carriage of origin-of-transfer (oriT) sequences mimicking those conjugative related pWBG749. pWBG749-family have diverged carry five distinct oriT subtypes and non-conjugative been identified that contain mimics each. The relaxasome accessory factor SmpO, encoded by each plasmid, determines specificity its cognate oriT. Here we...
Microbial biotransformation is valuable for obtaining pharmaceutical, nutritional, and cosmetical potential molecules. Basically, its realization, only a parental molecule, microorganism, suitable fermentation system are needed. Regarding the last requirement, most common resource used screenings shaken flasks (a.k.a., conical or Erlenmeyers). Despite their popularity, several concerning points discussed here limit utilization. Multiparametric bioreactors give more reliable results but...
Abstract Aim No oral niche can be considered to segregated from the subjacent milieu because of complex community behavior and nature biofilms. The aim this study was address paucity information on how these species are clonally related gingival crevice bacteria. Methods We utilized a metagenomic approach amplifying 16S rDNA genomic DNA, cloning, sequencing analysis using LIBSHUFF software assess genetic homogeneity bacterial two infected root canals crevices. Results four niches studied...
In 2010 a single isolate of trimethoprim-resistant multilocus sequence type 5, Panton–Valentine leucocidin-positive, community-associated methicillin-resistant Staphylococcus aureus (PVL-positive ST5 CA-MRSA), colloquially named WA121, was identified in northern Western Australia (WA). WA121 now accounts for ~14 % all WA MRSA infections. To gain an understanding the genetic composition and phylogenomic structure isolates we sequenced genomes 155 collected 2010–2021 present detailed genomic...