A5090133051- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Nitric Oxide and Endothelin Effects
Czech Academy of Sciences, Institute of Experimental Botany
2009-2023
Czech Academy of Sciences
2015
Zero to Three
2009
Charles University
2009
Plant roots navigate in the soil environment following gravity vector. Cell divisions meristem and rapid cell growth elongation zone propel root tips through soil. Actively elongating cells acidify their apoplast to enable wall extension by activity of plasma membrane AHA H+-ATPases. The phytohormone auxin, central regulator gravitropic response development, inhibits growth, likely rising pH apoplast. However, role auxin regulation apoplastic gradient along tip is unclear. Here, we show,...
Remarkable progress in various techniques of vivo fluorescence microscopy has brought an urgent need for reliable markers tracking cellular structures and processes. The goal this manuscript is to describe unexplored effects the FM (Fei Mao) styryl dyes, which are widely used probes that label processes endocytosis vesicle trafficking eukaryotic cells. Although there few reports on effect dyes membrane fluidity activity mammalian receptors, have been considered as tools plant endocytosis....
Processes of anterograde and retrograde membrane trafficking play an important role in cellular homeostasis dynamic rearrangements the plasma (PM) all eukaryotes. These processes depend on activity adenosine ribosylation factors (ARFs), a family GTP-binding proteins their guanine exchange (GEFs). However, knowledge function specificity individual ARF-GEFs for steps pathways is still limited plants. In this work, treatments with various inhibitors showed that endocytosis FM 4–64 largely...
Abstract Phosphatidylinositol 4-kinases (PI4Ks) are the first enzymes that commit phosphatidylinositol into phosphoinositide pathway. Here, we show Arabidopsis thaliana seedlings deficient in PI4Kβ1 and β2 have several developmental defects including shorter roots unfinished cytokinesis. The pi4kβ1β2 double mutant was insensitive to exogenous auxin concerning inhibition of root length cell elongation; it also responded more slowly gravistimulation. pi4kß1ß2 transcriptome displayed some...
Abstract Polar auxin transport in the Arabidopsis root tip maintains high levels around stem cell niche that gradually decrease dividing cells but increase again once they transition towards differentiation. Protophloem differentiates earlier than other proximal tissues and employs a unique ‘canalization’ machinery is thought to balance efflux with retention. It consists of proposed activator PIN carriers, AGC kinase PAX; its inhibitor, BRX; PIP5K enzymes, which promote polar PAX BRX...
Plant roots navigate in the soil environments following gravity vector. Cell divisions meristem and rapid cell growth elongation zone propel root tips through soil. Actively elongating cells acidify their apoplast to enable wall extension by activity of plasma membrane AHA H + -ATPases. The phytohormone auxin, central regulator gravitropic response development, inhibits growth, likely rising pH apoplast. However, role auxin regulation apoplastic gradient along tip is unclear. Here we show,...