A5024663682- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Gene Regulatory Network Analysis
- Protein Degradation and Inhibitors
- CAR-T cell therapy research
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- RNA Research and Splicing
- Epigenetics and DNA Methylation
University of California, Berkeley
2022-2024
University of California, San Francisco
2022
Bioengineering Center
2021
Stanford University
2021
In mammalian cells genes that are in close proximity can be transcriptionally coupled: silencing or activating one gene affect its neighbors. Understanding these dynamics is important for natural processes, such as heterochromatin spreading during development and aging, when designing synthetic regulation circuits. Here, we systematically dissect this process single by recruiting releasing repressive chromatin regulators at dual-gene reporters, measuring how fast reactivation spread a...
Gene therapies have the potential to treat disease by delivering therapeutic genetic cargo disease-associated cells. One limitation their widespread use is lack of short regulatory sequences, or promoters, that differentially induce expression delivered in target cells, minimizing side effects other cell types. Such cell-type-specific promoters are difficult discover using existing methods, requiring either manual curation access large datasets promoter-driven from both targeted and...
Epigenetic regulation involves the coordinated interplay of diverse proteins. To systematically explore these combinations, we present COMBINE (combinatorial interaction exploration), a high-throughput platform that tests over 50,000 pairs epigenetic effector domains up to 2,094 amino acids in length for their ability modulate endogenous human gene transcription. revealed synergistic and antagonistic interactions between protein domains, including potent KRAB-L3MBTL3 fusion enhanced...
The ability to deliver genetic cargo human cells is enabling rapid progress in molecular medicine, but designing this for precise expression specific cell types a major challenge. Expression driven by regulatory DNA sequences within short synthetic promoters, relatively few of these promoters are cell-type-specific. design cell-type-specific using model-based optimization would be impactful research and therapeutic applications. However, models from (promoter-driven expression) lacking most...
Abstract In mammalian cells genes that are in close proximity coupled transcriptionally: silencing or activating one gene can affect its neighbors. Understanding these dynamics is important for natural processes, such as heterochromatin spreading during development and aging, when designing synthetic regulation. Here, we systematically dissect this process single by recruiting releasing repressive chromatin regulators at dual-gene reporters, measuring how fast reactivation spread a function...