A5091649321- X-ray Diffraction in Crystallography
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Crystallization and Solubility Studies
- CRISPR and Genetic Engineering
- DNA Repair Mechanisms
- Nanoparticles: synthesis and applications
- Glycogen Storage Diseases and Myoclonus
- Genetics and Neurodevelopmental Disorders
- Natural Compound Pharmacology Studies
- Chromosomal and Genetic Variations
- Alcoholism and Thiamine Deficiency
- Mitochondrial Function and Pathology
- Biochemical Acid Research Studies
- Molecular Sensors and Ion Detection
- Trace Elements in Health
- Cancer Genomics and Diagnostics
- RNA Research and Splicing
- Neurological and metabolic disorders
- Laser Applications in Dentistry and Medicine
- Metabolism and Genetic Disorders
- Hair Growth and Disorders
- Lysosomal Storage Disorders Research
- Cellular Mechanics and Interactions
- Transgenic Plants and Applications
Indian Institute of Science Bangalore
2018-2024
The University of Texas Medical Branch at Galveston
2021-2024
Madurai Kamaraj University
2015-2019
Madurai Medical College
2015-2018
SASTRA University
2013
Overexpression of cysteine cathepsins proteases has been documented in a wide variety cancers, and enhances the l-cysteine concentration tumor cells. We report synthesis characterization copper(ii) complexes [Cu(L1)2(H2O)](SO3CF3)2, 1, L1 = 3-phenyl-1-(pyridin-2-yl)imidazo[1,5-a]pyridine, [Cu(L2)2(SO3CF3)]SO3CF3, 2, L2 3-(4-methoxyphenyl)-1-pyridin-2-yl-imidazo[1,5-a]pyridine, [Cu(L3)2(H2O)](SO3CF3)2, 3, L3 3-(3,4-dimethoxy-phenyl)-1-pyridin-2-yl-imidazo[1,5-a]pyridine...
Y-family DNA polymerases (Pols) are intrinsically highly error-prone; yet they<br />conduct predominantly error-free translesion synthesis (TLS) in normal human cells. In<br />response to damage, Pols assemble and function together with WRN, WRNIP1, Rev1 TLS. Among these proteins, WRN possesses a 3’→5’ exonuclease activity an ATPase/3’→5’ helicase activity, WRNIP1 has DNA-dependent ATPase activity. In previous study, we identified role of the high vivo fidelity TLS by Pols. Here...
Nanoparticle biosynthesis using the extract of medicinal plants in a non-hazardous mode has gained wide attention for various applications nanomedicine.
Nonsense mutations that result in premature stop codons the HBB gene cause β-thalassemia. This disease is characterized by a reduced hemoglobin level due to lack of β-globin. Compounds induce translational readthrough across thalassemia-causing codon will have therapeutic benefits. Currently available molecules specificity, and some them show toxicity after prolonged use. In this study, we developed an oligonucleotide-based approach codon. Oligonucleotides target mRNA downstream could...
By extending synthesis opposite from a diverse array of DNA lesions, polymerase (Pol) ζ performs crucial role in translesion (TLS). In yeast and cancer cells, Rev1 functions as an indispensable scaffolding component Polζ it imposes highly error-prone TLS upon Polζ. However, for that occurs during replication normal human instead Pols η, ι, κ Rev1-dependent by these operates predominantly error-free manner. The lack requirement function cells suggested some other protein substitutes this...
Abstract Stop codon readthrough (SCR) is the process where translation continues beyond a stop on an mRNA. Here, we describe strategy to enhance or induce SCR in transcript-selective manner using CRISPR-dCas13 system. Using specific guide RNAs, target dCas13 region downstream of canonical codons mammalian AGO1 and VEGFA mRNAs, known exhibit natural SCR. Readthrough assays reveal enhanced these mRNAs (both exogenous endogenous) caused by dCas13-gRNA complexes. This effect associated with...
Purified translesion synthesis (TLS) DNA polymerases (Pols) replicate through lesions with a low fidelity; however, TLS operates in predominantly error-free manner normal human cells. To explain this incongruity, here we determine whether Y family Pols, which play an eminent role replication diversity of lesions, are incorporated into multiprotein ensemble and the intrinsically high error rate Pol is ameliorated by components ensemble. end, provide evidence for indispensable Werner syndrome...
Quorum sensing has been implicated to control the biofilm formation and virulence factors among many bacteria.SdiA, a transcriptional regulator of uropathogenic E. coli quorum system that controls arsenal.Natural products offer unlimited opportunities for new drug leads because utmost availability chemical diversity.Here, potentiality five different extracts Melia dubia root inhibitory activity were investigated against Escherichia (UPEC).The assays such as cell density, swarming motility,...
Mutations in ALDH3A2 cause Sjögren-Larsson syndrome (SLS), a neuro-ichthyotic condition due to the deficiency of fatty aldehyde dehydrogenase (FALDH). We screened for novel mutations causing SLS among Indian ethnicity, characterized identified silico and vitro, retrospectively evaluated their role phenotypic heterogeneity. Interestingly, asymmetric distribution nonclassical traits was observed our cases. Nerve conduction studies suggested intrinsic-minus-claw hands two siblings, neurological...
Promoter polymorphism rs559748047 in<italic>G6PC1</italic>from GSD-1a among Indian population.
Abstract Stop codon readthrough (SCR) is the process where translation continues beyond a stop on an mRNA. Here, we describe strategy to enhance or induce SCR in transcript-selective manner using CRISPR-dCas13 system. Using specific guide RNAs, targeted dCas13 downstream region of canonical codons mammalian AGO1 and VEGFA, which are known exhibit natural SCR. Results assays revealed enhancement these mRNAs (both exogenous endogenous) caused by dCas13. This effect was associated with...