A5015444656- CRISPR and Genetic Engineering
- Insect Resistance and Genetics
- Insect symbiosis and bacterial influences
- Mosquito-borne diseases and control
- CAR-T cell therapy research
- Vector-Borne Animal Diseases
- Insect and Arachnid Ecology and Behavior
- Agricultural safety and regulations
- Advanced biosensing and bioanalysis techniques
Caribou Biosciences (United States)
2023-2024
Texas A&M University
2021-2022
Mitchell Institute
2021
Abstract Genetics-based pest management processes, including the sterile insect technique, are an effective method for control of some insects. However, current SIT methods not directly transferable to many important species due lack genetic sexing strains. Genome editing is revolutionizing way we conduct genetics in insects, Tribolium castaneum , model and agricultural pest. We identified orthologues β 2 Tubulin, Rad50-ATPase enolase T. castaneum. Using RT-PCR, confirmed that these genes...
Transgenic technology for mosquitoes is now more than two decades old, and a wide array of control sequences have been described regulating gene expression in various life stages or specific tissues. Despite this, comparatively little attention has paid to the development validation other transgene-regulating elements, especially 3ʹ-untranslated regions (3ʹUTRs). As consequence, same regulatory are often used multiple times single transgene array, potentially leading instability transgenic...
Background Aedes aegypti , the main vector of dengue, yellow fever, and other arboviruses thrives in tropical subtropical areas around globe putting half world’s population at risk. Despite aggressive efforts to control transmission those viruses, an unacceptable number cases occur every year, emphasizing need develop new strategies. Proposals for focused on suppression could offer a feasible alternative method reduce disease transmission. The induction extreme male-biased sex ratios has...
Mosquito gene editing has become routine in several laboratories with the establishment of systems such as transcription-activator-like effector nucleases (TALENs), zinc-finger (ZFNs), and homing endonucleases (HEs). More recently, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) technology offered an easier cheaper alternative for precision genome engineering. Following nuclease action, DNA repair pathways will fix broken ends, often...
Abstract Background: CLL-1 is a compelling therapeutic target for AML as it highly expressed on tumor cells and leukemic stem but not hematopoietic cells. CB-012 was engineered with next-generation Cas12a CRISPR hybrid RNA-DNA (chRDNA) genome-editing technology leverages both checkpoint disruption immune cloaking armoring strategies to potentially improve antitumor activity. The anti-CLL-1 CAR developed fully human scFv the CD28 costimulatory domain currently in development treatment of...
Abstract Background: CB-012 is an allogeneic anti-CLL-1 CAR-T cell therapy in development for evaluation relapsed or refractory acute myeloid leukemia (r/r AML). engineered with a next-generation CRISPR genome-editing technology to leverage both checkpoint disruption and immune cloaking enhance persistence of antitumor activity. Methods: Caribou’s hybrid RNA-DNA (chRDNA) guides combination Cas12a were used make five edits the manufacture CB-012. A fully human CAR transgene was...
Mosquito gene editing has become routine in several laboratories with the establishment of systems such as transcription-activator-like effector nucleases (TALENs), zinc-finger (ZFNs), and homing endonucleases (HEs). More recently, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) technology offered an easier cheaper alternative for precision genome engineering. Following nuclease action, DNA repair pathways will fix broken ends, often...