A5001047519- Herpesvirus Infections and Treatments
- Immune Cell Function and Interaction
- Influenza Virus Research Studies
- Mosquito-borne diseases and control
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Viral Infections and Vectors
- Poxvirus research and outbreaks
- Cytomegalovirus and herpesvirus research
- Dermatology and Skin Diseases
- Vector-borne infectious diseases
- interferon and immune responses
- Malaria Research and Control
- Virus-based gene therapy research
- Respiratory viral infections research
- Animal Virus Infections Studies
- Lung Cancer Treatments and Mutations
- RNA regulation and disease
- Biochemical and Molecular Research
- SARS-CoV-2 and COVID-19 Research
- Immune Response and Inflammation
- Curcumin's Biomedical Applications
- Lymphatic System and Diseases
- Viral Infections and Immunology Research
- Viral Infections and Outbreaks Research
National Taiwan University
2011-2024
Taipei Medical University
2012-2024
Taipei Institute of Pathology
2016-2022
Wan Fang Hospital
2012-2014
National Sun Yat-sen University
2012-2013
National Science and Technology Council
2012
Genomics Research Center, Academia Sinica
2012
Pediatrics and Genetics
2005-2008
Stanford University
2001-2007
Imperial College London
2006
Memory T cells maintain their numbers for long periods after antigen exposure. Here we show that CD8 + of memory phenotype divide slowly in animals. This division requires interleukin-15 and is markedly increased by inhibition interleukin-2 (IL-2). Therefore, the animals are controlled a balance between IL-15 IL-2.
Primary infection with varicella-zoster virus (VZV) causes the characteristic syndrome of varicella, or chickenpox. Experiments in severe combined immunodeficiency mice human skin grafts (SCIDhu mice) indicate that VZV T cells can mediate transfer infectious to skin. VZV-infected reached epithelial sites replication within 24 h after entering circulation. Memory CD4+ were predominant population recovered from SCIDhu given uninfected infected mononuclear cells, suggesting immune surveillance...
Objective: The synthesis of appropriate extracellular matrix by cells in tissue engineered heart valve constructs will be important for the maintenance cusp integrity and function. We have examined compared capacity mesenchymal stem to synthesise collagen response stretch comparison with native aortic interstitial cells.
ABSTRACT Varicella-zoster virus (VZV) is an alphaherpesvirus with the characteristic neurotropism of this group, but VZV also infects T cells productively and downregulates major histocompatibility complex (MHC) class I expression on infected cells, as shown in SCID-hu mouse model. T-cell tropism likely to be critical for cell-associated viremia associated primary infection. In these experiments, we found that human tonsillar CD4 + culture, 15 25% being positive proteins detected by...
Varicella-zoster virus (VZV) causes varicella and establishes latency in sensory ganglia. VZV reactivation results herpes zoster. We developed a model using human dorsal root ganglion (DRG) xenografts severe combined immunodeficient (SCID) mice to investigate infection of differentiated neurons satellite cells vivo . DRG engrafted under the kidney capsule contained within typical architecture. clinical isolates infected DRG. At 14 days postinfection, VZ virions were detected by electron...
Innate cellular immunity is the immediate host response against pathogens, and activation of innate also modulates induction adaptive immunity. The nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs) are a family intracellular that recognize conserved patterns associated with but information about their role in defense DNA viruses limited. Here we report varicella-zoster virus (VZV), an alphaherpesvirus causative agent varicella herpes zoster, induces formation NLRP3...
Vascular endothelial growth factor (VEGF)-C is recognized as a tumor lymphangiogenic based on the effects of activated VEGF-R3 lymphatic cells. Many cells express but function this receptor in largely unknown. It has been reported that VEGF-C/VEGF-R3 axis subsets leukemia patients. Herein, we have shown VEGF-C induces angiogenic activity tube formation assay invitro and Matrigel plug vivo by upregulating an factor, cyclooxygenase-2 (COX-2), through human acute myeloid (AML) cell line, THP-1....
The pathogenesis of varicella-zoster virus (VZV) involves a cell-associated viremia during which infectious is carried from sites respiratory mucosal inoculation to the skin. We now demonstrate that VZV infection T cells associated with robust virion production and modulation apoptosis interferon pathways within these cells. serine/threonine protein kinase encoded by ORF66 essential for efficient replication in Preventing expression stop codon insertion (pOka66S) impaired growth parent Oka...
Objective: Acute lung injury results in acute respiratory distress syndrome. There is no standard therapy for syndrome but supportive care. Stem cells offer a new therapeutic potential tissue regeneration as result of their self-renewal, multipotency, and paracrine capabilities. The objective this study to investigate the effects mechanisms systemic human orbital fat-derived stem/stromal cell transplantation on lipopolysaccharide-induced injury. Design: Prospective, randomized, controlled...
Chemotherapy is the main treatment for acute myeloid leukemia (AML), but cure rates AML patients remain low, and notorious adverse effects of chemotherapeutic drugs drastically reduce life quality patients. Penfluridol, a long-acting oral antipsychotic drug, has an outstanding safety record exerts oncostatic on various solid tumors. Until now, effect penfluridol remains unknown. cell lines harboring wild-type (WT) Fms-like tyrosine kinase 3 (FLT3) internal tandem duplication (ITD)-mutated...
Abstract Older humans and mice frequently contain very large clones of CD8+ T cells. In these cells are phenotypically similar to memory Like cells, most members the in continuous slow division, apparently independently Ag stimulation. Proliferation clonal is inhibited treated with Ab IL-2R β-chain that blocks signaling by either IL-2 or IL-15. However, inhibition increases numbers dividing Therefore, like normal expansion driven IL-15 probably limited amounts present host. Control two...
ABSTRACT Varicella-zoster virus (VZV) open reading frame 63 (ORF63), located between nucleotides 110581 and 111417 in the internal repeat region, encodes a nuclear phosphoprotein which is homologous to herpes simplex type 1 (HSV-1) ICP22 duplicated terminal region as ORF70 (nucleotides 118480 119316). We evaluated role of ORFs 70 VZV replication, using recombinant cosmids PCR-based mutagenesis make single dual deletions these ORFs. was recovered within 8 10 days when with were transfected...
Our earlier studies had suggested that endonuclease G (EndoG), a member of the evolutionarily conserved DNA/RNA nonspecific betabetaalpha-Me-finger nuclease family, functioned in sequence-mediated segment inversion observed during herpes simplex virus 1 replication. To test this hypothesis, we used RNA interference to reduce level EndoG mammalian cells culture. Reduction produced small but statistically significant decrease recombination, suggesting does play role process. We also reduction...
MxA is an antiviral protein induced by interferon (IFN)‐α/β that known to inhibit the replication of many RNA viruses. In these experiments, 76‐kDa expressed in IFN‐α‐treated cells was shown have activity against herpes simplex virus‐1 (HSV‐1), a human DNA virus. However, as 56‐kDa HSV‐1‐infected absence IFN‐α. This previously unrecognized isoform produced from alternatively spliced transcript had deletion Exons 14–16 and frame shift altering C‐terminus. The variant (varMxA) associated with...
Demethoxycurcumin (DMC) is a curcumin analogue with better stability and higher aqueous solubility than after oral ingestion has the potential to treat diverse cancers, including squamous cell carcinoma (OSCC). The aim of this study was investigate anticancer effects underlying mechanisms DMC against OSCC. We found that suppressed proliferation via simultaneously inducing G2/M-phase arrest apoptosis. Mechanistic investigations downregulation cellular IAP 1 (cIAP1)/X-chromosome-linked (XIAP)...
Abstract Current clinical trials of combined EGFR-tyrosine kinase inhibitors (TKI) and immune checkpoint blockade (ICB) therapies show no additional effect. This raises questions regarding whether EGFR-TKIs attenuate ICB-enhanced CD8+ T lymphocyte function. Here we that the EGFR-TKI afatinib suppresses proliferation, identify CAD, a key enzyme de novo pyrimidine biosynthesis, to be novel target. Afatinib reduced tumor-infiltrating numbers in Lewis lung carcinoma (LLC)–bearing mice. Early...
To investigate the role of ORF47 protein kinase varicella-zoster virus (VZV), we constructed VZV recombinants with targeted mutations in conserved motifs and a truncated characterized these mutants for replication, phosphorylation, protein-protein interactions vitro infectivity human skin xenografts SCID-hu mouse model vivo. Previous experiments showed that ROka47S, null mutant makes no protein, did not replicate vivo (J. F. Moffat, L. Zerboni, M. H. Sommer, T. C. Heineman, J. I. Cohen,...
Because 21 immunized children (13%) among the 162 confirmed Japanese encephalitis (JE) cases during 1986-1991 occurred in Taiwan, we collected 320 serum samples from Taiwan aged 15-31 and 27-44 months immediately before 1st dose (n = 41) 1-3 after 2nd 78, 27 pairs), 58) 3rd 143, 44 pairs) to determine neutralization antibody (Nt Ab) against Nakayama (N) Beijing-1 (B) strains two wild type JE viruses (JEV): CC-27 CH-1392. Our Nt results showed that (1) B vaccine stimulated a better homologous...
Abstract Introduction Our previous works demonstrated that systemic orbital fat-derived stem cell (OFSC) transplantation was effective in ameliorating lipopolysaccharide (LPS)-induced extensive acute lung injury (ALI) vivo mainly through paracrine regulation of macrophage-mediated cytokine-storm. In this study, we explore the molecular mechanism(s) OFSCs regulating macrophage activity a cytokine-inducible fashion. Methods LPS (100 ng/ml)-activated macrophages were treated by conditioned...
Curcumin (CUR) has a range of therapeutic benefits against cancers, but its poor solubility and low bioavailability limit clinical use. Demethoxycurcumin (DMC) diphenyl difluoroketone (EF-24) are natural synthetic curcumin analogues, respectively, with better solubilities higher anti-carcinogenic activities in various solid tumors than CUR. However, the efficacy these analogues non-solid tumors, particularly acute myeloid leukemia (AML), not been fully investigated. Herein, we observed that...