A5004018510- Multiple Myeloma Research and Treatments
- Viral-associated cancers and disorders
- Lymphoma Diagnosis and Treatment
- Peptidase Inhibition and Analysis
- Cytomegalovirus and herpesvirus research
- Parvovirus B19 Infection Studies
- Polyomavirus and related diseases
- Immune Cell Function and Interaction
- FOXO transcription factor regulation
- Protein Degradation and Inhibitors
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Chronic Lymphocytic Leukemia Research
- Abdominal Trauma and Injuries
- Cancer Mechanisms and Therapy
- Long-Term Effects of COVID-19
- Galectins and Cancer Biology
- Appendicitis Diagnosis and Management
- Erythrocyte Function and Pathophysiology
- Ubiquitin and proteasome pathways
- Monoclonal and Polyclonal Antibodies Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Dermatological and COVID-19 studies
- Hematological disorders and diagnostics
- COVID-19 Clinical Research Studies
- Trauma and Emergency Care Studies
University of Iowa Hospitals and Clinics
2018-2024
University of Iowa
2014-2022
University of Nebraska Medical Center
2020
Riyadh Armed Forces Hospital
2020
Indiana University – Purdue University Indianapolis
2020
Yale University
2020
University of Iowa Holden Comprehensive Cancer Center
2015
University of Minnesota
2009-2012
Twin Cities Orthopedics
2010
University of Washington
2008
Background. Characterizing virus‐host interactions during self‐limited infectious mononucleosis could explain how Epstein‐Barr virus (EBV) replication is normally controlled and provide insight into why certain immunocompromised patients fail to contain it. Methods. University students had an average of 7 clinical virologic evaluations acute mononucleosis. EBV was quantified in 697 samples oral wash fluid, whole blood, peripheral blood mononuclear cells (PBMCs), plasma by a real‐time...
Protein-protein interactions are key to function and regulation of many biological pathways. To facilitate characterization protein-protein using mass spectrometry, a new data acquisition/analysis pipeline was designed. The goal for this provide generic strategy identifying cross-linked peptides from single LC/MS/MS sets, without specialized cross-linkers or custom-written software. achieve this, each peptide in the pair considered be "post-translationally" modified with an unknown at amino...
We postulated that quantitative monitoring of Epstein-Barr virus (EBV) shedding after transplantation could distinguish EBV-associated illnesses and predict clinical outcome. EBV DNA was measured in solid organ (SOT) hematopoietic cell transplants (HCT) using our own real-time TaqMan PCR. The proportion patients who had DNAemia post-transplant significantly lower HCT vs. SOT (p < 0.001). Over a 7.5-yr period, lymphoproliferative disorder (PTLD) occurred 66 (5.8%) 1131 met adequate criteria....
Abstract Acute myeloid leukemia (AML) is maintained by self-renewing leukemic stem cells (LSCs). A fundamental problem in treating AML that conventional therapy fails to eliminate LSCs, which can reinitiate leukemia. Heat shock transcription factor 1 (HSF1), a central regulator of the stress response, has emerged as an important target cancer therapy. Using genetic Hsf1 deletion and direct HSF1 small molecule inhibitor, we show specifically required for maintenance AML, while sparing...
Wudhikarn K, Holman CJ, Linan M, Blaes AH, Dunitz JM, Hertz ME, Peterson BA. Post‐transplant lymphoproliferative disorders in lung transplant recipients: 20‐yr experience at the University of Minnesota. Clin Transplant 2011: 25: 705–713. © 2010 John Wiley & Sons A/S. Abstract: (PTLD) are potentially fatal complications solid organ transplantation. The natural history PTLD varies considerably among different types organs transplanted. While recipients highly susceptible to PTLD, there...
Following up on previous work demonstrating the involvement of transcription factor forkhead box M1 (FOXM1) in biology and outcome a high-risk subset newly diagnosed multiple myeloma (nMM), this study evaluated whether FOXM1 gene expression may be further upregulated upon tumor recurrence patients with relapsed (rMM). Also assessed was hypothesis that increased levels diminish sensitivity cells to commonly used drugs, such as proteasome inhibitor bortezomib (Bz) DNA intercalator doxorubicin (Dox).
Abstract: A 20‐year‐old patient, who received a bone marrow transplant in order to treat metachromatic leukodystrophy (MLD), succumbed cytomegalovirus (CMV) encephalitis. After CMV viremia developed, the patient ganciclovir, but he was switched foscarnet when ganciclovir resistance suspected. Foscarnet discontinued because of concern about its potential central nervous system toxicity. Autopsy samples brain and cerebrospinal fluid contained DNA with UL97 mutation (M460V) known confer...
KSHV-encoded vIL-6 collaborates with deregulated c-Myc to drive plasmablastic neoplasms in mice
Abstract The cytologic findings of an extranodal NK/T‐cell lymphoma (NKTCL) presenting as a large adrenal mass with leptomeningeal involvement diagnosed by CT‐guided fine‐needle aspiration and cerebrospinal fluid (CSF) cytology are described. 65‐year‐old Caucasian patient presented progressive headache multiple cranial nerve neuropathies. Magnetic resonance imaging showed enhancement surrounding the conus medullaris cauda equine, subsequent PET/CT demonstrated right gland mass. Fine‐needle...
Lymphomas showing both MYC/8q24 rearrangement and IGH@BCL2/t(14;18)(q32;q21), also referred to as “double‐hit” or “dual‐hit” lymphomas (DHL) are rare B‐cell malignancies with a germinal center immunophenotype heterogeneous cytologic histologic features. Such may arise de novo through transformation of follicular classified either “B‐cell lymphoma, unclassifiable, features intermediate between diffuse large lymphoma (DLBCL) Burkitt (BL)” (most commonly), DLBCL, or, rarely, B‐lymphoblastic...
Comparative genetic and biological studies on malignant tumor counterparts in human beings laboratory mice may be powerful gene discovery tools for blood cancers, including neoplasms of mature B-lymphocytes plasma cells such as Burkitt lymphoma (BL) multiple myeloma (MM). We used EMSA to detect constitutive NF-κB/STAT3 activity BL- MM-like that spontaneously developed single-transgenic IL6 (interleukin-6) or MYC (c-Myc) mice, double-transgenic IL6MYC mice. qPCR measurements analysis clinical...
Follicular helper T cell (TFH) markers are expressed in angioimmunoblastic T-cell lymphoma (AITL) and peripheral of the TFH phenotype (PTCL-TFH). However, differential expression coexpression these benign other malignant lymphoid proliferations have not been well studied.We performed programmed death-1 (PD-1), C-X-C motif chemokine ligand 13 (CXCL13), inducible costimulator (ICOS), CD10, B-cell 6 protein (BCL-6) immunohistochemistry on AITL, PTCL otherwise specified (PTCL-NOS), PTCL-TFH, or...
Congenital dyserythropoietic anemia (CDA) type‐1 is a rare genetic disorder of ineffective erythropoiesis, which manifests in macrocytic anemia. We report CDA1 patient who as newborn presented with and persistent pulmonary hypertension the (PPHN) requiring mechanical ventilation. Post‐infancy, developed acral dysmorphism pectus excavatum latter rarely found CDA1. Patient compound heterozygote for known maternal‐derived missense‐mutation (c.1796A > G/p.Asn589Ser) novel paternal‐derived...
ABSTRACT We report an instructive case of acute myeloid leukemia with histiocytic differentiation (acute leukemia) arising in a patient, 52-year-old woman history follicular lymphoma. The results genetic studies proved clonal relationship between the lymphoma and leukemic cells. To our knowledge, this is first transdifferentiation into modified base 5-methylcytosine (M5c)–like reported karyotype on transdifferentiated neoplasm.
ABSTRACT A semiquantitative PCR assay for the detection of BK virus in urine was developed using primers that specifically amplified but not JC virus. DNA extracted from through treatment with proteinase K followed by precipitation sodium acetate. Semiquantitation achieved amplifying serial dilutions (1:1, 1:10, 1:100, and 1:1,000) specimens. Each included both positive (stock previously patient urine) negative (no template) controls. sample interpreted as if any showed amplification...
Abstract: We developed an in vitro Epstein-Barr virus (EBV) drug susceptibility assay using P3HR1 cells or lymphoblastoid from subjects with infectious mononucleosis, which were grown the presence of various concentrations acyclovir (ACV), ganciclovir (GCV) R-9-[4-hydroxy-2-(hydroxymethyl)butyl]guanine (H2G) and 12-O-tetradecanoyl-phorbol-13-acetate (TPA). On day 7, total cellular DNA was extracted EBV detected in-house quantitative real-time polymerase chain reaction (PCR) method. All three...
Multiparametric flow cytometry is a useful tool for diagnosis of plasma cell (PC) dyscrasias and assessment minimal residual disease in myeloma (PCM). However, the immunophenotypic differences between clonal PCs PCM those monoclonal gammopathy undetermined significance (MGUS) as well correlation these cytometric markers with pertinent laboratory parameters have not been evaluated.We retrospectively identified all newly diagnosed treatment-naive MGUS patients 09/2014 06/2015 who underwent...