A5073319741- Chemical Synthesis and Analysis
- Genomics and Chromatin Dynamics
- Advanced biosensing and bioanalysis techniques
- Click Chemistry and Applications
- Advanced Proteomics Techniques and Applications
- RNA and protein synthesis mechanisms
- Virus-based gene therapy research
- Plant responses to water stress
- Epigenetics and DNA Methylation
- HIV Research and Treatment
- Dendrimers and Hyperbranched Polymers
- Tree-ring climate responses
- Nitric Oxide and Endothelin Effects
- Eicosanoids and Hypertension Pharmacology
- Protein Degradation and Inhibitors
- Biochemical and Molecular Research
- DNA Repair Mechanisms
- Peptidase Inhibition and Analysis
- Sulfur Compounds in Biology
- Single-cell and spatial transcriptomics
- Enzyme Structure and Function
- Plant Water Relations and Carbon Dynamics
- Antimicrobial Peptides and Activities
- Metalloenzymes and iron-sulfur proteins
The University of Texas at Austin
2020-2021
The Ohio State University
2011-2019
State Library of Ohio
2016
Hydraulic capacitance and water storage form a critical buffer against cavitation loss of conductivity within the xylem system. Withdrawal from in leaves, branches, stems, roots significantly impacts sap flow, stomatal conductance, transpiration. Storage quantities differ based on soil availability, tree size, wood anatomy density, drought tolerance, hydraulic strategy (anisohydric or isohydric). However, majority studies focus measurement conifers tropical species. We demonstrate novel...
Abstract C‐terminal peptide thioesters are an essential component of the native chemical ligation approach for preparation fully or semisynthetic proteins. However, efficient generation by Fmoc solid‐phase synthesis remains a challenge. The recent N ‐acylurea to thioester relies on deactivation one amine 3,4‐diaminobenzoic acid (Dbz) during SPPS. Here, we demonstrate that this results in formation side products through over‐acylation Dbz, particularly when applied Gly‐rich sequences. We find...
Nucleosomes contain ∼146 bp of DNA wrapped around a histone protein octamer that controls accessibility to transcription and repair complexes. Posttranslational modification (PTM) proteins regulates nucleosome function. To date, only modest changes in structure have been directly attributed PTMs. Histone residue H3(T118) is located near the dyad can be phosphorylated. This PTM destabilizes nucleosomes implicated regulation repair. Here, we report gel electrophoretic mobility, sucrose...
We report a hybrid solid-solution phase ligation approach that combines the efficiency of solid with solution in total synthesis modified histone proteins.
A thorough structure-property relationship study has produced much-improved fluorescence probes for nitric oxide surrogate, <italic>e.g.</italic> N<sub>2</sub>O<sub>3</sub>, compared to the original NO<sub>550</sub>.
The field of proteomics has expanded recently with more sensitive techniques for the bulk measurement peptides as well single-molecule techniques. One limiting factor some these methods is need multiple chemical derivatizations and highly pure proteins free contaminants. We demonstrate a solid-phase capture-release strategy suitable proteolysis, purification, subsequent modification peptides. use this resin on an HEK293T cell lysate perform one-pot capture, derivatization to survey peptide...
Eukaryotic DNA binding proteins must access genomic that is packaged into chromatin in vivo. During a productive infection, retroviral integrases (IN) similarly interact with to integrate the viral cDNA genome. Here we examine role of nucleosome unwrapping integrase search for target site. These studies utilized PFV intasomes are comprised tetramer IN two oligomers mimicking ends. Modified recombinant human histones were used generate nucleosomes increased rates at different regions....
Abstract The field of proteomics has expanded recently with more sensitive techniques for the bulk measurement peptides as well single-molecule techniques. One limiting factor some these methods is need multiple chemical derivatizations and highly pure proteins free contaminants. We demonstrate a solid-phase capture strategy suitable proteolysis, purification, subsequent modification peptides. use this resin on an HEK293T cell lysate perform one-pot capture, derivatization to generate...