A5012916170- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Click Chemistry and Applications
- Advanced biosensing and bioanalysis techniques
- Advanced Proteomics Techniques and Applications
- RNA modifications and cancer
- Chemical Synthesis and Analysis
- Genomics and Chromatin Dynamics
- Neurobiology and Insect Physiology Research
- Biochemical Analysis and Sensing Techniques
- Receptor Mechanisms and Signaling
- Monoclonal and Polyclonal Antibodies Research
- Cell Image Analysis Techniques
- Biotin and Related Studies
- Advanced Biosensing Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Enzyme Structure and Function
Stanford University
2025
The University of Texas at Austin
2020-2024
C-terminal domain (CTD) of RNA polymerase II is crucial for recruiting transcription regulators via specific post-translational modifications (PTM), especially phosphorylation. The hypothesis combination PTMs, or CTD code, that can allow precise and dynamic recruitment machinery highly attractive, yet the experimental evidence to support this has been scarce. Here, despite lacking antibodies combinatorial phosphorylation, we developed an innovative approach detects double phosphorylation...
Abstract The cell surface is a dynamic interface that controls cell-cell communication and signal transduction relevant to organ development, homeostasis repair, immune reactivity, pathologies driven by aberrant phenotypes. spatial organization of proteins central these processes. High-resolution fluorescence microscopy proximity labeling have advanced studies protein associations, but the complete proteome remains uncharted. In this study, we systematically mapped human T-lymphocytes...
Light scattering in biological tissue presents a significant challenge for deep vivo imaging. Our previous work demonstrated the ability to achieve optical transparency live mice using intensely absorbing dye molecules, which created red spectrum while blocking shorter-wavelength photons. In this paper, we extend capability across entire visible by employing molecules with strong absorption ultraviolet and sharp edges that rapidly decline upon entering spectrum. This new color-neutral...
The yeast Saccharomyces cerevisiae is powerful for studying human G protein-coupled receptors as they can be coupled to its mating pathway. However, some receptors, including the mu opioid receptor, are non-functional, which may due presence of fungal sterol ergosterol instead cholesterol. Here we engineer produce cholesterol and introduce diverse mu, delta, kappa create sensitive biosensors that recapitulate agonist binding profiles antagonist inhibition. Additionally, receptor variants,...
Protein phosphorylation is a key regulatory mechanism involved in nearly every eukaryotic cellular process. Increasingly sensitive mass spectrometry approaches have identified hundreds of thousands sites, but the functions vast majority these sites remain unknown, with fewer than 5% currently assigned function. To increase our understanding functional protein we developed an approach (phospho-DIFFRAC) for identifying phosphorylation-dependence assemblies systematic manner. A combination...
During eukaryotic transcription, RNA polymerase II undergoes dynamic post-translational modifications on the C-terminal domain (CTD) of largest subunit, generating an information-rich PTM landscape that transcriptional regulators bind. The phosphorylation Ser5 and Ser2 CTD heptad occurs spatiotemporally with stages, recruiting different to Pol II. To delineate protein interactomes at we reconstructed patterns
RNA polymerase II relies on a repetitive sequence domain (YSPTSPS) within its largest subunit to orchestrate transcription. While phosphorylation serine-2/serine-5 of the carboxyl-terminal heptad repeats is well established, threonine-4’s role remains enigmatic. Paradoxically, threonine-4 was only detected after transcription end sites despite functionally implicated in pausing, elongation, termination, and messenger processing. Our investigation revealed that detection obstructed by...
Methods for the selective labeling of biogenic functional groups on peptides are being developed and used in workflow both current emerging proteomics technologies, such as single-molecule fluorosequencing. To achieve successful with any one method requires that peptide fragments contain group which chemistry is designed. In practice, only two present every fragment regardless protein cleavage site, namely, an N-terminal amine a C-terminal carboxylic acid. Developing global-labeling...
The field of proteomics has expanded recently with more sensitive techniques for the bulk measurement peptides as well single-molecule techniques. One limiting factor some these methods is need multiple chemical derivatizations and highly pure proteins free contaminants. We demonstrate a solid-phase capture-release strategy suitable proteolysis, purification, subsequent modification peptides. use this resin on an HEK293T cell lysate perform one-pot capture, derivatization to survey peptide...
ABSTRACT The yeast Saccharomyces cerevisiae is a powerful tool for studying G protein-coupled receptors (GPCRs) as they can be functionally coupled to its pheromone response pathway. However, some exogenous GPCRs, including the mu opioid receptor, are non-functional in yeast, which may due presence of fungal sterol ergosterol instead animal cholesterol. We engineered produce cholesterol and introduced human creating an biosensor capable detecting peptide DAMGO at EC 50 62 nM opiate morphine...
Abstract Methods for the selective labeling of biogenic functional groups on peptides are being developed and used in workflow both current emerging proteomics technologies, such as single-molecule fluorosequencing. To achieve successful with any one method requires that peptide fragments contain group which chemistry is designed. In practice, only two present every fragment regardless protein cleavage site, namely, an N -terminal amine a C carboxylic acid. Developing global-labeling...
During eukaryotic transcription, RNA polymerase II undergoes dynamic post-translational modifications on the C-terminal domain (CTD) of largest subunit, generating an information-rich PTM landscape binding transcriptional regulators. The phosphorylation Ser5 and Ser2 CTD heptad occurs spatialtemporally with stages, recruiting different regulators to Pol II. To delineate protein interactomes at we reconstructed patterns in vitro. Our results showed that distinct are recruited stages...
Abstract The field of proteomics has expanded recently with more sensitive techniques for the bulk measurement peptides as well single-molecule techniques. One limiting factor some these methods is need multiple chemical derivatizations and highly pure proteins free contaminants. We demonstrate a solid-phase capture strategy suitable proteolysis, purification, subsequent modification peptides. use this resin on an HEK293T cell lysate perform one-pot capture, derivatization to generate...
ABSTRACT Protein phosphorylation is a key regulatory mechanism involved in nearly every eukaryotic cellular process. Increasingly sensitive mass spectrometry approaches have identified hundreds of thousands sites but the functions vast majority these remain unknown, with fewer than 5% currently assigned function. To increase our understanding functional protein we developed an approach for identifying phosphorylation-dependence assemblies systematic manner. A combination non-specific...