A5025572710- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Mass Spectrometry Techniques and Applications
- Viral Infections and Vectors
- Advanced Proteomics Techniques and Applications
- Hippo pathway signaling and YAP/TAZ
- interferon and immune responses
The University of Texas at Austin
2019-2023
During eukaryotic transcription, RNA polymerase II undergoes dynamic post-translational modifications on the C-terminal domain (CTD) of largest subunit, generating an information-rich PTM landscape that transcriptional regulators bind. The phosphorylation Ser5 and Ser2 CTD heptad occurs spatiotemporally with stages, recruiting different to Pol II. To delineate protein interactomes at we reconstructed patterns
The phosphorylation states of RNA polymerase II coordinate the process eukaryotic transcription by recruitment regulators. individual residues repetitive heptad C-terminal domain (CTD) biggest subunit are phosphorylated temporally at different stages transcription. Intriguingly, despite similar flanking residues, Ser2 and Ser5 in CTD heptads play dramatically roles. mechanism how kinases place on correct serine is not well understood. In this paper, we use biochemical assays, mass...
The critical role of site-specific phosphorylation in eukaryotic transcription has motivated efforts to decipher the complex patterns exhibited by carboxyl-terminal domain (CTD) RNA polymerase II. Phosphorylation remains a challenging post-translational modification characterize mass spectrometry owing labile phosphate ester linkage and low stoichiometric prevalence, two features that complicate analysis high-throughput MS/MS methods. Identifying sites represents one significant hurdle...
The C-terminal domain (CTD) of the largest subunit in eukaryotic RNA polymerase II has a repetitive heptad sequence Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7 which is responsible for recruiting transcriptional regulatory factors. seventh residues mammals are less conserved and subject to various post-translational modifications, but consequences such variations not well understood. In this study, we use ultraviolet photodissociation mass spectrometry, kinetic assays, structural analyses dissect how...
During eukaryotic transcription, RNA polymerase II undergoes dynamic post-translational modifications on the C-terminal domain (CTD) of largest subunit, generating an information-rich PTM landscape binding transcriptional regulators. The phosphorylation Ser5 and Ser2 CTD heptad occurs spatialtemporally with stages, recruiting different regulators to Pol II. To delineate protein interactomes at we reconstructed patterns in vitro. Our results showed that distinct are recruited stages...
The phosphorylation states of RNA polymerase II (Pol II) coordinate the process eukaryotic transcription by recruitment regulators. largest subunit pol consists a highly disordered C-Terminal Domain (CTD). Individual residues repetitive heptads (Consensus sequence - Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7) CTD are phosphorylated temporally at different stages transcription. Intriguingly, despite similar flanking residues, Ser2 and Ser5 in play dramatically roles. uncanny ability kinases to...