A5102878681- RNA Research and Splicing
- RNA regulation and disease
- RNA modifications and cancer
- interferon and immune responses
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
The University of Texas at Austin
2024
Zhejiang University
2024
Cytosolic double-stranded DNA surveillance by cyclic GMP-AMP synthase (cGAS)-Stimulator of Interferon Genes (STING) signaling triggers cellular senescence, autophagy, biased mRNA translation, and interferon-mediated immune responses. However, detailed mechanisms physiological relevance STING-induced senescence are not fully understood. Here, we unexpectedly found that interferon regulatory factor 3 (IRF3), activated during innate sensing, forms substantial endogenous complexes in the nucleus...
C-terminal domain (CTD) of RNA polymerase II is crucial for recruiting transcription regulators via specific post-translational modifications (PTM), especially phosphorylation. The hypothesis combination PTMs, or CTD code, that can allow precise and dynamic recruitment machinery highly attractive, yet the experimental evidence to support this has been scarce. Here, despite lacking antibodies combinatorial phosphorylation, we developed an innovative approach detects double phosphorylation...
RNA polymerase II relies on a repetitive sequence domain (YSPTSPS) within its largest subunit to orchestrate transcription. While phosphorylation serine-2/serine-5 of the carboxyl-terminal heptad repeats is well established, threonine-4’s role remains enigmatic. Paradoxically, threonine-4 was only detected after transcription end sites despite functionally implicated in pausing, elongation, termination, and messenger processing. Our investigation revealed that detection obstructed by...