A5079785216- Receptor Mechanisms and Signaling
- Biochemical Analysis and Sensing Techniques
- Neurotransmitter Receptor Influence on Behavior
- Neurobiology and Insect Physiology Research
- Cannabis and Cannabinoid Research
- Neuropeptides and Animal Physiology
- Monoclonal and Polyclonal Antibodies Research
The University of Texas at Austin
2019-2024
The yeast Saccharomyces cerevisiae is powerful for studying human G protein-coupled receptors as they can be coupled to its mating pathway. However, some receptors, including the mu opioid receptor, are non-functional, which may due presence of fungal sterol ergosterol instead cholesterol. Here we engineer produce cholesterol and introduce diverse mu, delta, kappa create sensitive biosensors that recapitulate agonist binding profiles antagonist inhibition. Additionally, receptor variants,...
Yeast expression of human G-protein-coupled receptors (GPCRs) can be used as a biosensor platform for the detection pharmaceuticals. Cannabinoid receptor type 1 (CB1R) is particular interest, given cornucopia natural and synthetic cannabinoids being explored therapeutics. We show first time that engineering N-terminus CB1R allows efficient signal transduction in yeast, sterol composition yeast membrane modulates its performance. Using an engineered cannabinoid biosensor, we demonstrate large...
ABSTRACT The yeast Saccharomyces cerevisiae is a powerful tool for studying G protein-coupled receptors (GPCRs) as they can be functionally coupled to its pheromone response pathway. However, some exogenous GPCRs, including the mu opioid receptor, are non-functional in yeast, which may due presence of fungal sterol ergosterol instead animal cholesterol. We engineered produce cholesterol and introduced human creating an biosensor capable detecting peptide DAMGO at EC 50 62 nM opiate morphine...
G protein-coupled receptors (GPCRs) are a large and diverse group of membrane proteins that constitute over 30% FDA approved drug targets. Despite their importance, much remains unknown about GPCR signaling at system's level. Efforts to engineer with orthogonal components have attempted provide tools parse resultant phenotypes. Recent advances in synthetic biology opportunities scale additional properties could further inform level, enhance the ability complex signal transduction.
Yeast expression of human G Protein Coupled Receptors (GPCRs) can be used as a biosensor platform for the detection pharmaceuticals. The Cannabinoid receptors type 1 and 2 (CB1/2R) are particular interest, given cornucopia natural synthetic cannabinoids being explored therapeutics. We show first time that engineering N-terminus CB1R allows efficient signal transduction in yeast, sterol composition yeast membrane optimizes CB2R performance. Using dual cannabinoid biosensors, large libraries...