A5101440305- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Plant Gene Expression Analysis
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- HIV Research and Treatment
- Photosynthetic Processes and Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Plant Stress Responses and Tolerance
- HIV/AIDS drug development and treatment
- Genomics and Chromatin Dynamics
- Legume Nitrogen Fixing Symbiosis
- Identification and Quantification in Food
- MicroRNA in disease regulation
- Genomics and Phylogenetic Studies
- Cancer Genomics and Diagnostics
- Medicinal Plants and Neuroprotection
- Cancer Cells and Metastasis
- Plant-Microbe Interactions and Immunity
- Ubiquitin and proteasome pathways
- Plant Parasitism and Resistance
- Plant Virus Research Studies
- Light effects on plants
- Advanced NMR Techniques and Applications
- Atomic and Subatomic Physics Research
Chinese University of Hong Kong
2010-2023
Hong Kong Baptist University
2014-2018
The University of Texas at Austin
2009-2017
University of Pennsylvania
2006-2011
Institute of Molecular and Cell Biology
2010
Howard Hughes Medical Institute
2007
Texas A&M University
2004-2007
National Institute of Allergy and Infectious Diseases
2007
Columbia University
2007
National Institutes of Health
2007
Histone modifications regulate gene expression in eukaryotes, but their effects on transcriptomes of a multicellular organism and transcriptomic divergence between species are poorly understood. Here we present the first nucleotide-resolution maps histone acetylation, methylation, core Arabidopsis thaliana comprehensive analysis these all other available with data A. thaliana, arenosa , allotetraploids. H3K9 acetylation (H3K9ac) H3K4 trimethylation (H3K4me3) correlated, distribution patterns...
Abstract MicroRNAs (miRNAs) play essential roles in plant and animal development, but the cause effect of miRNA expression divergence between closely related species interspecific hybrids or allopolyploids are unknown. Here, we show differential regulation a miR163-mediated pathway allotetraploids their progenitors, Arabidopsis thaliana arenosa. miR163 is recently evolved A. highly expressed thaliana, its was undetectable arenosa repressed resynthesized allotetraploids. Repression MIR163 (Aa...
NBD-556 and the chemically structurally similar NBD-557 are two low-molecular weight compounds that reportedly block interaction between HIV-1 envelope glycoprotein gp120 its receptor, CD4. binds to with a binding affinity of 2.7 × 105 M-1 (Kd = 3.7 μM) in process characterized by large favorable change enthalpy partially compensated unfavorable entropy change, thermodynamic signature observed for sCD4 gp120. is associated structuring molecule, as also demonstrated CD spectroscopy. NBD-556,...
Hybrid plants and animals often show increased levels of growth fitness, a phenomenon known as hybrid vigor or heterosis. Circadian rhythms optimize physiology metabolism in animals. In plant hybrids polyploids, expression changes the genes within circadian regulatory network, such CIRCADIAN CLOCK ASSOCIATED1 (CCA1), lead to However, relationship between allelic CCA1 heterosis has remained elusive. Here, we parent-of-origin effect on altered Arabidopsis thaliana F1 hybrids. This biomass...
Summary WRKY transcription factors (TFs) belong to a large family of regulatory proteins in plants that modulate many plant processes. Extensive studies have been conducted on WRKY‐mediated defense response Arabidopsis thaliana and several crop species. Here, we aimed investigate the potential roles contributions TFs improving resynthesized allotetraploids ( suecica ) derived from two related autotetraploid progenitors , (At4) arenosa (Aa). Rapid differential induction WRKY18 WRKY40...
The phaseolin (phas) promoter drives copious production of transcripts encoding the protein during seed embryogenesis but is silent in vegetative tissues, which a nucleosome positioned over its three-phased TATA boxes. Transition from inactive state transgenic Arabidopsis thaliana leaves was accomplished by ectopic expression transcription factor Phaseolus vulgaris ABI3-like (ALF) and application abscisic acid (ABA). Placement hemagglutinin-tagged ALF under control an estradiol-inducible...
A detailed description of the construction and use a device for hyperpolarization select contrast agents is presented. The based on molecular incorporation spin‐order inherent to parahydrogen, followed by order transfer metastable heteronuclear alignment. Design considerations experimental results relating catalyst/solvent choice handling, solvent heating, efficient gas entrainment are described. resulting degree shown be substantial, ranging from few over 50%, depending target molecule....
The first step in HIV infection is the binding of envelope glycoprotein gp120 to host cell receptor CD4. An interfacial "Phe43 cavity" gp120, adjacent residue Phe43 gp120-bound CD4, has been suggested as a potential target for therapeutic intervention. We designed CD4 mutant (D1D2F43C) site-specific coupling compounds screening against cavity. Altogether, 81 cysteine-reactive were designed, synthesized, and tested. Eight derivatives exceeded affinity native D1D2 gp120. Structure−activity...
In plants, many mRNAs and non-coding RNAs are cleaved by RNA-induced silencing complexes. After cleavage, only a limited number of processed into trans-acting siRNAs (tasiRNAs). One reason is that 22 nt small RNAs, but not the more common 21 can efficiently trigger tasiRNA formation. The characteristics target transcripts may also affect production. Here we report effects site location sequence complementarity on A synthetic included miR173 two targeting an endogenous mRNA encoding PHYTOENE...
Abstract Small RNAs are important regulators for a variety of biological processes, including leaf development, flowering-time, embryogenesis and defense responses. miR163 is non-conserved miRNA its locus has evolved recently through inverted duplication target genes to which they belong the SABATH family related small-molecule methyltransferases (MTs). In Arabidopsis thaliana , previous study demonstrated that accumulation was induced by alamethicin treatment, suggesting roles in response...
Malaria is widely associated with poverty, and a low-cost vaccine against malaria highly desirable for implementing comprehensive vaccination programmes in developing countries. Production of antigens plants promising approach, but its development has been hindered by poor expression the plant cells. In present study, we targeted seeds as production platform successfully expressed Plasmodium falciparum 42-kDa fragment merozoite surface protein 1 (MSP1₄₂), leading candidate, at high level...
• Both natural and newly synthesized allopolyploids display nonadditive gene expression changes through genetic epigenetic mechanisms. The nonadditively expressed genes include many microRNA (miRNA) targets, suggesting a role for miRNAs their targets in morphological variation the progenitors. We produced dominant-negative transgenic allotetraploid plants Arabidopsis using RNA interference (RNAi) that downregulates of miRNA biogenesis genes, including DCL1 AGO1. RNAi AGO1 led to dominant...
Abstract Genomic interactions in allopolyploids create expression variation of homoeologous alleles through protein-protein and protein-DNA interactions. However, the molecular basis for this is largely unknown. Here we investigated among transcription factors circadian-clock feedback loop, consisting CCA1 HIKING EXPEDITION (CHE), CIRCADIAN CLOCK ASSOCIATED1 (CCA1), TIMING OF CAB EXPRESSION1 (TOC1), plus interaction with a chromatin factor, HISTONE DEACETYLASE1 (HD1). In allotetraploids...
Abstract Systemic acquired resistance (SAR) is an induced immune mechanism in plants, involving epigenetic regulation by chromatin remodeling and DNA methylation, which can be inherited to progeny following stress exposure. Intersexual conflict sometimes leads unequal expression of maternal paternal alleles offspring, resulting parent-of-origin effects inheritance. To better understand the parental contributions epialleles plant defense, isogenic Arabidopsis lines were mock-treated (M)...