A5065054276- Advanced biosensing and bioanalysis techniques
- Advanced Fluorescence Microscopy Techniques
- Photosynthetic Processes and Mechanisms
- DNA and Nucleic Acid Chemistry
- Lipid Membrane Structure and Behavior
- Alzheimer's disease research and treatments
- Advanced Electron Microscopy Techniques and Applications
- Antioxidant Activity and Oxidative Stress
- Biotin and Related Studies
- Spectroscopy and Quantum Chemical Studies
- RNA Research and Splicing
- Caveolin-1 and cellular processes
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Nanofabrication and Lithography Techniques
- Microfluidic and Bio-sensing Technologies
- Protein Kinase Regulation and GTPase Signaling
- Molecular Junctions and Nanostructures
- Receptor Mechanisms and Signaling
- Electrochemical Analysis and Applications
- Cardiovascular and Diving-Related Complications
- Erythrocyte Function and Pathophysiology
- Nanopore and Nanochannel Transport Studies
- Ginkgo biloba and Cashew Applications
- Photoreceptor and optogenetics research
Vilnius University
2020-2025
Czech Academy of Sciences, Institute of Biotechnology
2023-2025
Center for Physical Sciences and Technology
2017-2024
University of Copenhagen
2012-2024
Lundbeck Foundation
2013-2015
Electron transfer between membrane spanning oxidoreductase enzymes controls vital metabolic processes. Here we studied for the first time with single molecule resolution function of P450 (POR), canonical activator all microsomal cytochrome enzymes. Measurements and statistical analysis individual catalytic turnover cycles shows POR to sample at least two major functional states. This phenotype may underlie regulatory interactions different cytochromes but date has remained masked in bulk...
Abstract Nanoparticles and nanomaterials are revolutionizing medicine by offering diverse tools for diagnosis therapy, including devices, contrast agents, drug delivery systems, adjuvants, therapeutics, theragnostic agents. Realizing full applied potential requires a deep understanding of the interactions nano dimensional objects with biological cells. In this study, we investigate interaction single-crystal diamond nanoneedles (SCDNNs) containing silicon vacancy (SiV-) colour centres...
Heme-copper oxidases (HCOs) are key enzymes in prokaryotes and eukaryotes for energy production during aerobic respiration. They catalyze the reduction of terminal electron acceptor, oxygen, utilize Gibbs free to transport protons across a membrane generate proton (ΔpH) electrochemical gradient termed motive force (PMF), which provides driving adenosine triphosphate (ATP) synthesis. Excessive PMF is known limit turnover HCOs, but molecular mechanism this regulatory feedback remains...
Commercial super-resolution (SR) imaging systems require a high budget, while current more affordable open source microscopy lack modularity and sometimes are too complex or reliability. We present miEye – cost-effective microscope designed for high-resolution wide-field fluorescence imaging. The build is constructed using CNC milled aluminum body commercially available optomechanics, with open-source Python-based control, data visualization, analysis software integration. acquisition works...
Protein liquid-liquid phase separation (LLPS) is a rapidly emerging field of study on biomolecular condensate formation. In recent years, this phenomenon has been implicated in the process amyloid fibril formation, serving as an intermediate step between native protein transition into their aggregated state. The formation fibrils via LLPS demonstrated for number proteins related to neurodegenerative disorders, well other amyloidoses. Despite surge amyloid-related studies, influence end-point...
Reconstitution of transmembrane proteins into liposomes is a widely used method to study their behavior under conditions closely resembling the natural ones. However, this approach does not allow precise control liposome size, reconstitution efficiency, and actual protein-to-lipid ratio in formed proteoliposomes, which might be critical for some applications and/or interpretation data acquired during spectroscopic measurements. Here, we present novel strategy employing methods proteoliposome...
Calcium-binding S100A8 and S100A9 proteins play a significant role in various disorders due to their pro-inflammatory functions. Substantially, they are also relevant neurodegenerative via the delivery of signals for immune response. However, at same time, can aggregate accelerate progression diseases. Natively, exist as homo- heterodimers, but upon aggregation, form amyloid-like oligomers, fibrils, or amorphous aggregates. In this study, we aimed elucidate aggregation propensities S100A8,...
The photosynthetic apparatus of plants is a robust self-adjustable molecular system, able to function efficiently under varying environmental conditions. Under strong sunlight, it switches into photoprotective mode avoid overexcitation by safely dissipating the excess absorbed light energy via nonphotochemical quenching (NPQ). Unfortunately, heterogeneous organization and simultaneous occurrence multiple processes within thylakoid membrane impede study natural NPQ in vivo conditions; thus,...
The DNA Curtains assay is a recently developed experimental platform for protein–DNA interaction studies at the single-molecule level that based on anchoring and alignment of fragments. so far have been made by using chromium barriers fluid lipid bilayer membranes, which makes such specialized technically challenging relatively unstable. Herein, we report an alternative strategy arraying analysis individual DNA–protein interactions. It relies stable tethering onto nanopatterned protein...
G-protein-coupled receptors (GPCRs) are structurally flexible membrane proteins that mediate a host of physiological responses to extracellular ligands like hormones and neurotransmitters. Fine features their dynamic structural behavior hypothesized encode the functional plasticity seen in GPCR activity, where with different efficacies can direct same receptor toward signaling phenotypes. Although number crystal structures is increasing, characterized by complex poorly understood...
Nonphotochemical quenching (NPQ) is the major self-regulatory mechanism of green plants, performed on a molecular level to protect them from an overexcitation during direct sunlight. It believed that NPQ becomes available due conformational dynamics light-harvesting photosynthetic complexes and involves participation carotenoids. In this work, we perform single-molecule microscopy (LHCII) different Arabidopsis thaliana mutants exhibiting various carotenoid composition. We show how distinct...
Over the past 20 years, single-molecule methods have become extremely important for biophysical studies. These methods, in combination with new nanotechnological platforms, can significantly facilitate experimental design and enable faster data acquisition. A platform, which utilizes a flow-stretch of immobilized DNA molecules, called Curtains, is one best examples such combinations. Here, we employed strategies to fabricate assay stably oriented molecules using protein template-directed...
Many type II restriction endonucleases require two copies of their recognition sequence for optimal activity. Concomitant binding DNA sites by such an enzyme produces a loop. Here we exploit single-molecule Förster resonance energy transfer (smFRET) surface-immobilized fragments to study the dynamics looping induced tetrameric endonuclease NgoMIV. We have employed fragment with NgoMIV and FRET dye pair that upon protein-induced dyes are brought close proximity resulting in signal. The...
Abstract In the course of cancer progression tumor cells undergo morphological changes that lead to increased motility and invasiveness thus promoting formation metastases. This process called epithelial mesenchymal transition (EMT) is triggered by transforming growth factor (TGFβ) but for gaining full invasive potential an interplay between signaling TGFβ Ras GTPases required. proteins possess a lipidated domain mediates association with plasma membrane, which essential biological...
Protein-DNA interactions are the core of cell’s molecular machinery. For a long time, conventional biochemical methods served as powerful investigatory basis protein-DNA and target search mechanisms. Currently single-molecule (SM) techniques have emerged complementary tool for studying these revealed plenty previously obscured mechanistic details. In comparison to traditional ones, SM allow direct monitoring individual biomolecules. Therefore, reveal reactions that otherwise hidden by...
Abstract CRISPR-Cas systems are prokaryotic adaptive immune that protect against phages and other invading nucleic acids. The evolutionary arms race between prokaryotes gave rise to phage anti-CRISPR (Acr) proteins act as a counter defence by inhibiting the effector complex. Here, we used combination of bulk biochemical experiments, X-ray crystallography single-molecule techniques explore inhibitory activity AcrIF6 AcrIF9 type I-F system from Aggregatibacter actinomycetemcomitans (Aa). We...
Liquid-liquid phase separation (LLPS) of proteins and nucleic acids is a rapidly emerging field study, aimed at understanding the process biomolecular condensate formation its role in cellular functions. LLPS has been shown to be responsible for generation promyelocytic leukemia protein bodies, stress granules, intrinsically disordered condensates. Recently, it discovered that different neurodegenerative disease-related proteins, such as alpha-synuclein (related Parkinson's disease)...