A5052352974- Synthesis and Characterization of Heterocyclic Compounds
- Organophosphorus compounds synthesis
- DNA and Nucleic Acid Chemistry
- Biochemical and Molecular Research
- HIV/AIDS drug development and treatment
- Click Chemistry and Applications
- Monoclonal and Polyclonal Antibodies Research
- Sulfur-Based Synthesis Techniques
- Chemical Synthesis and Reactions
- Nanoparticle-Based Drug Delivery
- Advanced biosensing and bioanalysis techniques
- Chemical Synthesis and Analysis
- Fluorine in Organic Chemistry
- Carbohydrate Chemistry and Synthesis
- Chemical Synthesis and Characterization
- Educational Robotics and Engineering
- Quinazolinone synthesis and applications
- Pneumocystis jirovecii pneumonia detection and treatment
- Trace Elements in Health
- RNA and protein synthesis mechanisms
- Genetics, Bioinformatics, and Biomedical Research
- Peptidase Inhibition and Analysis
- Bacteriophages and microbial interactions
Queen's University Belfast
2015-2024
University of Ulster
2019
Belfast City Hospital
2019
BTG International (United Kingdom)
2015
Exploiting the rapid kinetics and low solvent requirements of mechanochemically-activated reactions, instability phosphorothiolate monoesters was bypassed enabling one-pot hydrolytic desilylation phosphate coupling to be achieved in a ball mill.
Oligodeoxynucleotides incorporating internucleotide phosphoroselenolate linkages have been prepared under solid-phase synthesis conditions using dimer phosphoramidites. These dimers were constructed following the high yielding Michaelis-Arbuzov (M-A) reaction of nucleoside H-phosphonate derivatives with 5'-deoxythymidine-5'-selenocyanate and subsequent phosphitylation. Efficient coupling phosphoramidites to solid-supported substrates was observed both manual automated required only minor...
Novel nucleoside analogues containing photoswitchable moieties were prepared using ‘click’ cycloaddition reactions between 5′-azido-5′-deoxythymidine and mono- or bis-N-propargylamide-substituted azobenzenes. In solution, high to quantitative yields achieved 5 mol% Cu(I) in the presence of a stabilizing ligand. ‘Click’ monopropargylamides also effected absence added cuprous salts by application liquid assisted grinding (LAG) metallic copper reaction vials. Specifically, speed vibration ball...
Recent studies in colorectal cancer patients (CRC) have shown that increased resistance to thymidylate synthase (TS) inhibitors such as 5-fluorouracil (5-FU), reduce the efficacy of standard care (SoC) treatment regimens. The nucleotide pool cleanser dUTPase is highly expressed CRC and an attractive target for potentiating anticancer activity chemotherapy. purpose current work was investigate P1, P4-di(2′,5′-dideoxy-5′-selenouridinyl)-tetraphosphate (P4-SedU2), a selenium-modified...
Vibration ball-milling in a zirconia-lined vessel afforded clean and quantitative nucleophilic displacement reactions between 4-methoxybenzylthiolate salts nucleoside 5′-halides or 5′-tosylates five to 60 minutes. Under these conditions, commonly-encountered cyclisation byproducts (especially of purine nucleosides) were not observed. Liquid-assisted grinding the same 5'-iodide 5′-tosylate substrates with potassium selenocyanate presence DMF produced corresponding 5′-selenocyanates variable...
Abstract Using vibration ball milling, 5′‐chloro‐5′‐deoxyadenosine (CldA) reacts cleanly with 4‐methoxybenzyl mercaptan (MobSH), under basic conditions, to the corresponding thioether (MobSdA), which is isolated following precipitation and trituration. Under acidic in a one‐pot, two‐step process, MobSdA transformed into 5′‐deoxy‐5′‐(5‐nitropyridyl‐2‐disulfanyl)‐adenosine (NPySSdA). Michaelis‐Arbuzov (M‐A) reaction of NPySSdA tris(trimethylsilyl) phosphite proceeds completion within 30 min as...
Novel sulfur and selenium substituted 5′,5′-linked dinucleoside pyrophate analogues were prepared in a vibration ball mill from the corresponding persilylated monophosphate. The chemical hydrolysis of pyrophosphorochalcogenolate-linked dimers was studied over wide pH-range. effect chalcogeno-substitution on reactivity pyrophosphates surprisingly modest, stability is promising considering potential therapeutic or diagnostic applications. precursor phosphorochalcogenolate monoesters also...