A5001809780- Mitochondrial Function and Pathology
- Liver Disease Diagnosis and Treatment
- Metabolism and Genetic Disorders
- Adipose Tissue and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Lipid metabolism and biosynthesis
- SARS-CoV-2 and COVID-19 Research
- Metabolomics and Mass Spectrometry Studies
- Pediatric Urology and Nephrology Studies
- Neonatal Health and Biochemistry
- Lipid Membrane Structure and Behavior
- Posttraumatic Stress Disorder Research
- ATP Synthase and ATPases Research
- Synthetic Organic Chemistry Methods
- Autophagy in Disease and Therapy
- Toxoplasma gondii Research Studies
- Biochemical Acid Research Studies
- Retinal Diseases and Treatments
- Amyotrophic Lateral Sclerosis Research
- Vector-borne infectious diseases
- biodegradable polymer synthesis and properties
- Gut microbiota and health
- Migration, Health and Trauma
- SARS-CoV-2 detection and testing
- Coenzyme Q10 studies and effects
Kwantlen Polytechnic University
2025
University of California, Los Angeles
2017-2025
Children's Hospital of Philadelphia
2015
Institute of Infection and Immunity
2012
Serologic biomarkers for inflammatory bowel disease (IBD) have yielded variable differentiating ability. Quantitative analysis of a large number metabolites is promising method to detect IBD biomarkers. Human subjects with active Crohn's (CD) and ulcerative colitis (UC) were identified, serum, plasma, urine specimens obtained. We characterized 44 37 71 by use (1)H NMR spectroscopy "targeted analysis" differentiate between diseased non-diseased individuals, as well the CD UC cohorts. used...
Changes in mitochondrial morphology are associated with nutrient utilization, but the precise causalities and underlying mechanisms remain unknown. Here, using cellular models representing a wide variety of shapes, we show strong linear correlation between fragmentation increased fatty acid oxidation (FAO) rates. Forced elongation following MFN2 over-expression or DRP1 depletion diminishes FAO, while forced upon knockdown knockout augments FAO as evident from respirometry metabolic tracing....
Significance More than 170 mutations in superoxide dismutase 1 (SOD1) are linked to inherited forms of ALS, and aggregates this protein a pathological feature associated with disease. Although it is accepted that SOD1 gains toxic function the disease state, molecular understanding species lacking. Here, we identify short segment both necessary sufficient for toxicity motor neurons. The crystal structure reveals an out-of-register β-sheet oligomer, providing structural rationale effects mutant ALS.
The mitochondrial fission protein Drp1 was proposed to promote NAFLD, as inhibition of hepatocyte early in life prevents liver steatosis induced by high-fat diet mice. However, whether Drp1-knockdown older mice can reverse established NASH is unknown. N-acetylgalactosamine-siRNA conjugates, an FDA approved method deliver siRNA selectively hepatocytes, were used knockdown hepatocyte-Drp1 (NAG-Drp1si). C57BL/6NTac Gubra-Amylin-NASH (D09100310, 40% fat, 22% fructose and 2% cholesterol)...
Abstract Measuring oxygen consumption allows for the role of mitochondrial function in biological phenomena and diseases to be determined. Although respirometry has become a common approach disease research, current methods are limited by necessity process measure tissue samples within 1 hr acquisition. Detailed Acin‐Perez colleagues, new designed previously frozen eliminates these hurdles study. This technique measurement maximal respiratory capacity long‐term storage before testing....
Although the role of hydrophilic antioxidants in development hepatic insulin resistance and nonalcoholic fatty liver disease has been well studied, lipophilic remains poorly characterized. A known hydrogen peroxide scavenger is bilirubin, which can be oxidized to biliverdin then reduced back bilirubin by cytosolic reductase. Oxidation inside mitochondria must followed export cytosol, where bilirubin. Thus, putative mitochondrial exporter expected a major determinant regeneration...
Abstract Parkin, an E3 ubiquitin ligase, plays essential role in mitochondrial quality control. However, the mechanisms by which Parkin connects homeostasis with cellular metabolism adipose tissue remain unclear. Here, we demonstrate that Park2 gene (encodes Parkin) deletion specifically from protects mice against high-fat diet and aging-induced obesity. Despite a mild reduction mitophagy, DNA content function are increased deficient white adipocytes. Moreover, elevates biogenesis increasing...
SARS-CoV-2 can infect cells through endocytic uptake, a process that is targeted by inhibition of lysosomal proteases. However, clinically this approach to treat viral infections has afforded mixed results, with some studies detailing an oral regimen hydroxychloroquine accompanied significant off-target toxicities. We rationalized organelle-targeted will avoid toxicity while increasing the concentration drug at target. Here, we describe lysosome-targeted, mefloquine-loaded poly(glycerol...
Proteins released by the club-shaped, apically located, specialized secretory organelles called rhoptries play an essential role in host cell invasion and intracellular survival of apicomplexans. Sarcocystis neurona, apicomplexan responsible for equine protozoal myeloencephalitis (EPM), lacks its asexual developmental stages, viz., merozoites schizonts. Nevertheless, rhoptry protein (ROP) homologues were detected S. neurona transcriptome proteome, SnROP9 was particularly abundant. In this...
Public health officials reported increases in stigma, discrimination, and verbal physical abuse during the coronavirus (COVID-19) pandemic. This study, conducted Vietnam, examined how fear of virus, self-protective behaviors, threats to loss resources pandemic were associated with post-traumatic stress belief vaccine effectiveness. Participants 380 persons (237 women, 129 men, 14 unreported) who completed measures assessing demographics, stigma experienced pandemic, resource loss, about...
Coenzyme Q (Qn) is a vital lipid component of the electron transport chain that functions in cellular energy metabolism and as membrane antioxidant. In yeast Saccharomyces cerevisiae, coq1–coq9 deletion mutants are respiratory-incompetent, sensitive to peroxidation stress, unable synthesize Q6. The coq10 mutant also respiratory-deficient peroxidation, yet it continues produce Q6 at an impaired rate. Thus, Coq10 required for function respiration antioxidant believed chaperone from its site...
Mitochondria play a central role in lipid metabolism and can bind to droplets. However, the functional specialization of population peridroplet mitochondria (PDMs) remain unclear, as methods isolate PDMs were not developed until recently. Here, we describe an approach intact from murine brown adipose tissue based on their adherence isolated using our be used study specialized function by respirometry. For complete information use execution this protocol, please refer Benador et al. (2018).
ABSTRACT SARS-CoV-2 can infect cells through endocytic uptake, a process which is targeted by inhibition of lysosomal proteases. However, clinically this approach to treat viral infections has afforded mixed results, with some studies detailing an oral regimen hydroxychloroquine accompanied significant off-target toxicities. We rationalized that organelle-targeted will avoid toxicity while increasing the concentration drug at target. Here we describe lysosome-targeted, mefloquine-loaded...
Abstract As the SARS-CoV-2 coronavirus continues to evolve and infect global population, many individuals are likely suffer from post-acute sequelae of infection (PASC). Manifestations PASC include vision symptoms, but little is known about ability impact retinal cells. Here, we demonstrate that can perturb pigment epithelium (RPE) in vivo, after intranasal inoculation a transgenic mouse model infection, cell culture. Separate lentiviral studies showed Spike protein mediates viral entry...