We detected large numbers of HLA-DR-positive reactive microglia (macrophages), along with Lewy bodies and free melanin, in the substantia nigra all cases studied Parkinson9s disease (5) parkinsonism dementia (PD) (5). found similar, but less extensive, pathology six nine Alzheimer type (DAT) only one 11 age-matched nonneurologic cases. All a premortem diagnosis DAT or PD showed significant plaque tangle counts hippocampus, as well reduced cortical choline acetyltransferase activity. One...

Abstract The need to identify protein or peptide biomarkers via readily available biological samples like serum, plasma, cerebrospinal fluid is often hindered by a few particular proteins present at relatively high concentrations. ability remove these specifically, reproducibly, and with selectivity increasingly important in proteomic studies, success this procedure leading an ever‐increasing list of lower abundant being identified fluids. current work addresses some the potential problems...

Abstract A central feature of the inflammatory pathology in Alzheimer’s disease is activated microglia clustered around aggregated amyloid β (Aβ) peptide-containing plaques. In vitro-cultured can be to an state by Aβ with induction a range different neurotoxic factors and provide model system for studying interactions. Gene expression responses human postmortem brain-derived were measured using whole genome microarrays address hypothesis that interactions primarily induce proinflammatory...

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTSize Exclusion Chromatography and Related Separation TechniquesHoward G. Barth, Barry E. Boyes, Christian JacksonView Author Information Central Research Development, DuPont Company, Experimental Station, P.O. Box 80228, Wilmington, Delaware 19880-0228, Little Falls Analytical DivisionNewport, Hewlett-Packard 538 First State Boulevard, Newport, 19804, Marshall Laboratory, Automative Products, 3401 Grays Ferry Avenue, Philadelphia, Pennsylvania 19146...

Serum analysis represents an extreme challenge due to the dynamic range of proteins interest, and high structural complexity constituent proteins. In serum, quantities peptides interest from those considered "high abundance", present at 2-70% by mass total protein, "low 10(-12) M or less. This analytical target molecules is outside realm available technologies for proteomic analysis. Therefore, in this study, we have developed a workflow toward addressing these samples through application...

The advent of superficially porous particles (SPPs) for packed HPLC columns has changed the way that many practitioners have approached problem developing needed separations. very high efficiency such columns, combined with convenient operating conditions, modest back pressures and ability to use conventional instruments resulted in intense basic studies SPP technology, widespread applications sciences. This report contains an overview technology first developed 2006 by Advanced Materials...

The ability to resolve glycans while attached tryptic peptides would greatly facilitate glycoproteomics, as this enable site-specific glycan characterization. Peptide/glycopeptide separations are typically performed using reversed-phase liquid chromatography (RPLC), where retention is driven by hydrophobic interaction. As the hydrophilic do not interact significantly with RPLC stationary phase, it difficult glycopeptides that differ only in their structure, even when these differences large....

Abstract: The metabolism of the positron‐emitting compound [ 18 F]6‐fluoro‐l‐3,4‐dihydroxyphenylalanine (*F‐DOPA) was studied in carbidopa‐pretreated male hooded rats. Thirty minutes following carbidopa administration (5 mg/kg i.p.), animals received *F‐DOPA (500 μg/kg; specific activity, 175–230 Ci/mol) as an intrajugular bolus. Blood samples were taken at various times between 5 and 90 min, plasma analyzed by HPLC with gamma counting fractions. disappeared rapidly from concert formation 3‐...

The study of N-linked glycans is among the most challenging bioanalytical tasks because their complexity and variety. presence glycoform families that differ only in branching and/or linkage position makes identification quantitation individual exceedingly difficult. Quantitation these important changes abundance isomers are often associated with significant biomedical events. For instance, previous studies have shown ratio α2-3 to α2-6 linked sialic acid (SA) plays an role cancer biology....

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPhysicochemical and optical studies on the calcium- potassium-induced conformational changes in bovine brain S-100b proteinRajam S. Mani, Barry E. Boyes, Cyril M. KayCite this: Biochemistry 1982, 21, 11, 2607–2612Publication Date (Print):May 25, 1982Publication History Published online1 May 2002Published inissue 25 1982https://pubs.acs.org/doi/10.1021/bi00540a005https://doi.org/10.1021/bi00540a005research-articleACS PublicationsRequest reuse...

Fused-core superficially porous particles have recently created considerable interest for high-performance liquid chromatography separations because of their unusual high column efficiency and much lower back pressure when compared to sub-2-microm particles. With particles, larger solutes can move rapidly in out a thin shell, resulting reduced band broadening at higher mobile phase velocities greater separation speeds. The original silica fused-core were 2.7 microm diameter with 0.5-microm...

Some patients with the Zollinger-Ellison syndrome appear to have hypergastrinaemia and hyperplasia of antral G cells but no tumour. This subgroup has been classified as type 1. We treated such a patient by vagotomy antrectomy, fasting plasma gastrin acid secretion subsequently returning normal.A 17-year-old male had four-year history duodenal ulcer. Gastric tests showed hypersecretion. Fasting was 8350 pg/ml (normal 50-170 pg/ml). At laparotomy ulceration confirmed pancreatic or other...

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTSize Exclusion ChromatographyHoward G. Barth, Barry E. Boyes, and Christian JacksonView Author Information DuPont Company, Central Research Development, Experimental Station, P.O. Box 80228, Wilmington, Delaware 19880-0228, Rockland Technologies, Inc., 538 First State Boulevard, Newport, 19804 Cite this: Anal. Chem. 1996, 68, 12, 445–466Publication Date (Web):June 15, 1996Publication History Published online15 June 1996Published inissue 1 January...

A major challenge facing current mass spectrometry (MS)-based proteomics research is the large concentration range displayed in biological systems, which far exceeds dynamic of commonly available spectrometers.One approach to overcome this limitation improve online reversed-phase liquid chromatography (RP-LC) separation methodologies.LC mobile-phase modifiers are used peak shape and increase sample load tolerance.Trifluoroacetic acid (TFA) a modifier, as it produces peptide separations that...

Proteomic analysis of complex samples can be facilitated by protein fractionation prior to enzymatic or chemical fragmentation combined with MS-based identification peptides. Although aqueous soluble liquid chromatography is relatively straightforward, membrane separations have a variety technical challenges. Reversed-phase high performance (RP-HPLC) proteins often exhibit poor recovery and bandwidths, generally require extensive pretreatment remove lipids other components. Human brain...

Thirty-four healthy blood donors, found to be persistent HBAg carriers, have been investigated by means of serial liver function tests, bromsulphthalein (BSP) retention, and biopsy. Thirty-one the donors had histological abnormalities including one with cirrhosis, three chronic aggressive hepatitis, 11 hepatitis. In 13 biopsies there were focal areas necrosis in parenchyma. Serial tests revealed each cirrhosis or seven parenchymal necrosis. The degree BSP retention was greatest (>11%)...