A5028568920- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- Molecular Biology Techniques and Applications
- Bacterial Genetics and Biotechnology
- Glycosylation and Glycoproteins Research
- Microbial Metabolic Engineering and Bioproduction
- Physical Unclonable Functions (PUFs) and Hardware Security
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Electrostatic Discharge in Electronics
- Gene expression and cancer classification
- Integrated Circuits and Semiconductor Failure Analysis
- Migration and Labor Dynamics
- DNA and Nucleic Acid Chemistry
- Advanced Biosensing Techniques and Applications
- Protein purification and stability
- Cryptographic Implementations and Security
- RNA Interference and Gene Delivery
- Genomics and Phylogenetic Studies
- Animal Genetics and Reproduction
- Metabolomics and Mass Spectrometry Studies
- Biofuel production and bioconversion
- Fungal and yeast genetics research
Abcam (United States)
2016-2022
AxioMx (United States)
2012-2021
Novartis (Austria)
2020
Technical University of Munich
2012-2019
Sabin Vaccine Institute
2018
Conseil de L'Europe
2018
International Centre for Migration Policy Development
2018
John Wiley & Sons (United States)
2014
Bioengineering Center
2014
National Center for Genetic Engineering and Biotechnology
2014
The proliferation of large-scale DNA-sequencing projects in recent years has driven a search for alternative methods to reduce time and cost. Here we describe scalable, highly parallel sequencing system with raw throughput significantly greater than that state-of-the-art capillary electrophoresis instruments. apparatus uses novel fibre-optic slide individual wells is able sequence 25 million bases, at 99% or better accuracy, one four-hour run. To achieve an approximately 100-fold increase...
The purpose of this study was to use serial imaging gain insight into the sequence pathologic events in Alzheimer's disease, and clinical features associated with sequence. We measured change amyloid deposition over time using (11)C Pittsburgh compound B (PIB) positron emission tomography progression neurodegeneration structural magnetic resonance imaging. studied 21 healthy cognitively normal subjects, 32 amnestic mild cognitive impairment 8 disease. Subjects were drawn from two...
A rapid, high throughput readout for single-nucleotide polymorphism (SNP) analysis was developed employing single base chain extension and cytometric of an array fluorescent microspheres. An microspheres coupled with uniquely identifying sequences, termed complementary ZipCodes (cZipCodes), which allowed multiplexing possibilities. For a given assay, querying polymorphic involved extending oligonucleotide containing both ZipCode SNP-specific sequence DNA polymerase pair fluoresceinated...
BACKGROUND We have developed a rapid, high throughput method for single nucleotide polymorphism (SNP) genotyping that employs an oligonucleotide ligation assay (OLA) and flow cytometric analysis of fluorescent microspheres. METHODS A fluoresceinated reporter sequence is added to "capture" probe by OLA. Capture probes are designed hybridize both genomic "targets" amplified polymerase chain reaction separate complementary DNA has been coupled microsphere. These sequences on the capture called...
We have developed a rapid, cost-effective, high-throughput readout for single nucleotide polymorphism (SNP) genotyping using flow cytometric analysis performed on Luminex 100 cytometer. This robust technique employs PCR-derived target DNA containing the SNP, synthetic SNP-complementary ZipCode-bearing capture probe, fluorescent reporter molecule, and thermophilic polymerase. An array of microspheres, covalently coupled with complementary ZipCode sequences (cZipCodes), was hybridized to...
Large-scale human genotyping requires technologies with a minimal number of steps, high accuracy, and the ability to automate at reasonable cost. In this regard, we have developed rapid, cost-effective readout method for single nucleotide polymorphism (SNP) that combines an easily automatable single-tube allele-specific primer extension (ASPE) efficient throughput flow cytometric analysis performed on Luminex 100 cytometer. This robust technique employs ASPE reaction using PCR-derived target...
The substrate specificity of human collagenase 3 (MMP-13), a member the matrix metalloproteinase family, is investigated using phage-displayed random hexapeptide library containing 2 × 10<sup>8</sup> independent recombinants. A total 35 phage clones that express peptide sequence can be hydrolyzed by recombinant catalytic domain are identified. translated DNA these reveals highly conserved putative P1, P2, P3 and P1′, P2′, P3′ subsites substrates. Kinetic analysis synthetic substrates made...
Secret key generation with Physical Unclonable Functions (PUFs) is an alternative to conventional secure storage non-volatile memory.In a PUF, secret bits are generated by evaluating the internal state of physical source. Typically, error correction applied in two stages remove instability measurement that caused environmental influences.We present new syndrome coding scheme, called Differential Sequence Coding (DSC), for first stage. DSC applies fixed reliability criterion and searches PUF...
Microprobing allows intercepting data from on-chip wires as well injecting faults into or control lines. This makes it a commonly used attack technique against security-related semiconductors, such smart card controllers. We present the low area probing detector (LAPD) an efficient approach to detect microprobing. It compares delay differences between symmetric lines bus timing asymmetries introduced by capacitive load of probe. Compared with state-of-the-art microprobing countermeasures...
Introduction Syphilis, a sexually transmitted infection caused by the spirochete Treponema pallidum ( Tp ), is resurging globally. ’s repertoire of outer membrane proteins (OMPs) includes BamA (β-barrel assembly machinery subunit A/TP0326), bipartite protein consisting 16-stranded β-barrel with nine extracellular loops (ECLs) and five periplasmic POTRA (polypeptide transport-associated) domains. ECL4 antisera promotes internalization rabbit peritoneal macrophages. Methods Three overlapping...
Journal Article Polishing with T4 or Pfu polymerase increases the efficiency of cloning PCR fragments Get access Gina L. Costa, Costa Stratagene Cloning Systems11099 North Torrey Pines Road, La Jolla, CA 92037, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Michael P. Weiner * To whom correspondence should be addressed Nucleic Acids Research, Volume 22, Issue 12, 25 June 1994, Page 2423, https://doi.org/10.1093/nar/22.12.2423 Published: 1994 history...
We have altered the antibiotic resistance of reporter plasmids and pJG4-5 activation-domain pEG202 DNA binding-domain used in Brent interaction trap/two-hybrid system. These were each previously ampicillin-resistant, resulting an inefficient purification any one plasmid from a yeast strain containing all three that constitute complete trap. By creating derivatives these expressing either kanamycin or chloramphenicol resistance, along with parent plasmids, we now option to use trap E. coli...
The synthesis of the bovine pancreatic ribonuclease A (RNase A, EC 3.1.27.5) chromogenic substrate urldinine-3′-(5-bromo-4-chloroindol-3-yl)-phosphate (U-3-BCIP) is described. RNase catalyzes hydrolysis U-3-BCIP to release a halogenated indol-3-ol that undergoes rapid aerobic oxidation dark blue 5,5′-dibromo-4,4′-dichloroindlgo. Preliminary kinetic studies indicate this compound may have practical use for assaying activity both in vitro and vivo, e.g. screening bacterial colonies produced by...