A5022948834- Advanced biosensing and bioanalysis techniques
- Advanced Nanomaterials in Catalysis
- Electrochemical sensors and biosensors
- Biosensors and Analytical Detection
- Electrochemical Analysis and Applications
- Gold and Silver Nanoparticles Synthesis and Applications
- DNA and Nucleic Acid Chemistry
- Nanocluster Synthesis and Applications
- Quantum Dots Synthesis And Properties
- Diatoms and Algae Research
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- Adsorption, diffusion, and thermodynamic properties of materials
- Advanced Photocatalysis Techniques
- Bacteriophages and microbial interactions
- Copper-based nanomaterials and applications
- Metalloenzymes and iron-sulfur proteins
- Metallurgical Processes and Thermodynamics
- Chemical Synthesis and Analysis
- Nanoparticles: synthesis and applications
- Synthesis and Biological Evaluation
- Metal complexes synthesis and properties
- 3D Printing in Biomedical Research
- Ferrocene Chemistry and Applications
- Advanced Biosensing Techniques and Applications
CIC biomaGUNE
2014-2025
Boeing (Spain)
2025
Digital Research Alliance of Canada
2025
Siberian State Industrial University
2022
Federal Research Center of Fundamental and Translational Medicine
2020
Ayuntamiento de San Sebastián
2016-2018
Centre for Electrochemical Technologies
2015
Vologda State University
2008
Hebrew University of Jerusalem
2004-2006
Heidelberg University
2006
The catalytic enlargement of aptamer-functionalized Au nanoparticles amplifies the optical detection aptamer-thrombin complexes in solution and on surfaces.
A G-rich nucleic acid sequence binds hemin and yields a biocatalytic complex (DNAzyme) of peroxidase activity, namely, the biocatalyzed generation chemiluminescence in presence H2O2 luminol. The DNAzyme is used as label for amplified detection DNA, or analysis telomerase activity cancer cells, using an output signal. In one configuration, analyzed DNA hybridized with primer that associated Au surface, surface-confined analyte DNA. limit ∼1 × 10-9 M. second system, from HeLa cells induces...
DNAzyme-functionalized Au−NPs act as catalytic labels for the amplified detection of DNA and telomerase activity on nucleic acid-functionalized gold surface. The DNAzyme stimulates, in presence hemin, H2O2, luminol, generation chemiluminescence. For analysis, a acid unit complementary to analyzed is tethered structure associated with Au−NPs. activity, telomer repeat units generated surface limit 1 × 10-10 M. method enables originating from 1000 HeLa cells.
The acetylcholine esterase, AChE, mediated hydrolysis of acetylthiocholine (1) yields a reducing agent thiocholine (2) that stimulates the catalytic enlargement Au NP seeds in presence AuCl4-. reductive NPs is controlled by concentration substrate and activity enzyme. growth inhibited 1,5-bis(4-allyldimethylammoniumphenyl)pentane-3-one dibromide (3) or diethyl p-nitrophenyl phosphate (paraoxon; 4), thus enabling colorimetric test for AChE inhibitors. assay was also developed on glass supports.
Enzymatic oxidation of gold nanorods by the enzymatic activity horseradish peroxidase.
Illuminating DNA: The assembly of two tailored nucleic acids (colored blue and brown in the scheme) presence hemin yields a G-quadruplex that exhibits peroxidase-like activity catalyzes generation chemiluminescence H2O2 luminol. DNAzyme is employed for analysis DNA (green) solution on surfaces.
Gold-plated gold: NAD(P)H cofactors mediate the catalytic growth of Au nanoparticles in presence AuCl4− and CTAB (cetyltrimethylammonium bromide; see picture). The process allows for optical detection analysis NAD(P)+-dependent biocatalyzed transformations solutions on surfaces.
A conjugate consisting of a thrombin aptamer tethered to the thrombin, Th, with sensing nucleic acid (1) is used for optical detection DNA. The thrombin/aptamer complex blocks biocatalytic functions Th. Hybridization analyte DNA (2) 1 yields rigid duplex that detaches from process activates protein toward hydrolysis bis(p-tosyl-Gly-Pro-Arg)-R110 (3) rhodamine 110 fluorophore (4). system allows sensitivity limit × 10-8 M. aptamer/Th also immobilized on glass slides dissociation upon...
Aminoferrocene-based prodrugs are activated under cancer-specific conditions (high concentration of reactive oxygen species, ROS) with the formation glutathione scavengers (p-quinone methide) and ROS-generating iron complexes. Herein, we explored three structural modifications these in an attempt to improve their properties: (a) attachment a -COOH function ferrocene fragment leads improvement water solubility reactivity vitro but also decreases cell-membrane permeability biological activity,...
In this paper, we present the development of a highly sensitive, specific and reproducible nanobiosensor to detect one liver cancer biomarker, manganese super oxide dismutase (MnSOD). The high sensitivity reproducibility was reached by using SERS on gold nanostructures (nanocylinders coupled nanorods) produced electron-beam lithography (EBL). specificity detection provided use aptamer with affinity targeted protein as recognition element. With such sensor, have been able observe signal MnSOD...
In this study, a simple fluorogenic immunoassay based on in situ formation of semiconductor quantum dots (QDs) is described. We discovered that alkaline phosphatase (ALP), the enzyme broadly used enzyme-linked immuno-sorbent assay (ELISA), able to trigger fluorescent CdS QDs. ALP-catalyzed hydrolysis p-nitrophenyl phosphate (pNPP) leads p-nitrophenol and inorganic phosphate. The latter stabilizes QDs produced through interaction Cd(2+) with S(2-) ions. So, specific analyte (antibody)...
The anisotropic morphology of gold nanorods (AuNRs) has been shown to lead nonuniform ligand distribution and preferential etching through their tips. We have recently demonstrated that this effect can be achieved by biocatalytic oxidation with hydrogen peroxide, catalyzed the enzyme horseradish peroxidase (HRP). report here modification AuNRs thiol-containing organic molecules such as glutathione thiocholine hinders enzymatic AuNR etching. Higher concentrations in reaction mixture gradually...
Hydrolysis of acetylthiocholine mediated by acetylcholine esterase yields the thiol-bearing compound thiocholine. At trace concentrations, thiocholine modulates growth Au−Ag nanoparticles on seeding gold in presence ascorbic acid. Inhibition enzyme 1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide (BW284c51) or diethyl p-nitrophenyl phosphate (paraoxon) produces lower thiocholine, promoting catalytic nanoparticles. Here, we describe development a simple and sensitive colorimetric...
Glutathione is the most abundant nonprotein molecule in cell and plays an important role many biological processes, including maintenance of intracellular redox states, detoxification, metabolism. Furthermore, glutathione levels have been linked to several human diseases, such as AIDS, Alzheimer disease, alcoholic liver cardiovascular diabetes mellitus, cancer. A novel concept bioanalysis introduced applied highly sensitive inexpensive detection reduced (GSH), over its oxidized form (GSSG),...
Fuel for a DNA machine: A FokI enzyme/DNA scission machine is triggered by the hybridization of its hairpin structure with target nucleic acid. The fueled secondary fluorophore (F)- and quencher (Q)-functionalized acid 2. Scission 3 leads to replication machinery cutter 1 yields fluorescent product 6, which provides an optical output (see scheme). Supporting information this article available on WWW under http://www.wiley-vch.de/contents/jc_2002/2006/z503810_s.pdf or from author. Please...
We have developed an analytical assay to detect the enzymatic activity of acetylcholine esterase and alkaline phosphatase based on generation quantum dots by products. Acetylcholine converts acetylthiocholine into thiocholine. The latter enhances rate decomposition sodium thiosulfate H(2)S, which in presence cadmium sulfate yields CdS showing a time dependent exponential growth, typical autocatalytic processes. This was also applied inhibitors. Alkaline hydrolyzes thiophosphate instantly...
Abstract Three innovative assays are developed for the detection of enzymatic activities glucose oxidase (GOx) and horseradish peroxidase (HRP) by generation CdS quantum dots (QDs) in situ using non‐conventional reactions. In first assay, GOx catalyzes oxidation 1‐thio‐β‐ D ‐glucose to give ‐gluconic acid. The latter is spontaneously hydrolyzed β‐D‐gluconic acid H 2 S, which presence cadmium nitrate yields fluorescent nanoparticles. second assay HRP sodium thiosulfate with hydrogen peroxide...
Copper is an essential element for regulation of many biological processes, however, in excess it considered to be toxic human health. This metal frequently accompanied by other elements such as cadmium, nickel and lead. Thus, developing a selective simple method determination copper matrix containing heavy ions great importance. In this work, novel detection was developed using electrodes modified with the DNAzyme capturing Cu2+ ions. The reconstituted catalyzes oxidation ascorbic acid...
In the early years of 21st century, numerous viral infectious diseases have proliferated, prompting intensified efforts to devise more effective diagnostic methods. response, various biosensors emerged with aim overcoming constraints conventional techniques. Nanomaterial-based revolutionized approaches, significantly enhancing biosensor performance and effectively tackling these challenges. A diverse array nanoparticles nanomaterials has been systematically synthesized, engineered, employed...
Au/Cu core/shell nanoparticle assemblies on modified quartz or ITO slides are achieved through a biochemical reduction process, in which 1,4-dihydro-β-nicotinamide adenine dinucleotide (NADH) performs the Au-catalyzed of Cu2+ ions (see scheme). Such ideal for optical electrochemical detection NADH and NAD+-dependent biocatalytic processes, such as analysis ethanol.