A5026818585- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Monoclonal and Polyclonal Antibodies Research
- Cervical Cancer and HPV Research
- Molecular Biology Techniques and Applications
- CRISPR and Genetic Engineering
- Cancer Mechanisms and Therapy
- Hepatitis B Virus Studies
- Cancer Research and Treatments
- Hepatitis C virus research
- Advanced biosensing and bioanalysis techniques
- Cytomegalovirus and herpesvirus research
- Peptidase Inhibition and Analysis
- Mycobacterium research and diagnosis
- FOXO transcription factor regulation
- Tuberculosis Research and Epidemiology
- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- Protein purification and stability
- Parvovirus B19 Infection Studies
- Hepatitis Viruses Studies and Epidemiology
- Animal Genetics and Reproduction
- Viral Infectious Diseases and Gene Expression in Insects
- Mechanisms of cancer metastasis
- Blood properties and coagulation
Shiraz University of Medical Sciences
2016-2025
Shahid Beheshti University of Medical Sciences
2014
Purpose: This research investigated the development of short hairpin RNA (shRNA) molecules designed to target specific regions human respiratory syncytial virus (HRSV) M and F genes. The study aimed assess therapeutic potential these shRNAs evaluate effectiveness Tat peptide-mediated delivery in enhancing their functionality. Methods: We acquired isolates from pediatric patients experiencing illness then cultured HEp-2 cells. constructed plasmids expressing shRNAs. peptide as a facilitator...
Hypothyroidism due to Hashimoto's thyroiditis (HT) is the commonest autoimmune endocrine illness in which antibodies against thyroid organ result inflammation. The disease has a complex etiology that involves genetic and environmental influences. Viral infections may be involved triggering of as their molecular mimicry enhance responses. Human herpesvirus-6 (HHV-6) recognized for its contribution some diseases. In current study, prevalence HHV-6 active infection patients with HT...
Prostate cancer (PCa) is the most frequently diagnosed and second common cause of related mortality in United States male population. ScFv fragments have different usefulness. For example they small size, high perfusion rate, yield production are non-immunogenic, thus can be used for therapeutic purposes. In this project we a synthetic human library isolation monoclonal antibodies against prostate specific membrane antigen. purpose, after five rounds cell-panning, also antigen-panning with...
The nonstructural protein (NS1) of human parvovirus B19 (hPVB19) is considered to be a double-edged sword in its pathogenesis. NS1 promotes cell death by apoptosis erythroid-lineage cells and also implicated triggering the progression various inflammation autoimmune disorders. We investigated possible role hPVB19 modulation proinflammatory cytokines nonpermissive HEK-293T cells. A plasmid containing fully sequenced gene (pCMV6-AC-GFP-NS1) was transfected into Transfection efficiency assessed...
Background: Pseudomonas aeruginosa is a Gram negative ubiquitous opportunistic organism and one of the more problematic drug-resistant pathogens encountered today. Objectives: The aims current multicenter research were to assess antibiotic resistance profiles, carbapenemase production, detection IMP gene as well virulence factors genes including exoA, algD, lasB, plcH among clinical isolates P. aeruginosa. Methods: A total 80 nonduplicate recovered from inpatients. Bacterial identification...
Background: The p28 is a small-sized cell-penetrating peptide derived from bacterial protein azurin and can function as cancer-specific anti-proliferative agent. It penetrate cancer tissues easily without involving the immune system, increase intracellular concentration of p53. Objectives: In this study, we have expressed purified recombinant p28, then evaluated its pro-apoptotic effects on Raji cell line. Methods: gene was amplified cloned into pTZ57R cloning vector sequenced subsequently....
Antigen presentation using bacterial surface display systems, on one hand, has the benefits of carriers, including low-cost production and ease manipulation. On other bacteria can help in stimulating immune system as an adjuvant. For example, technology, we developed a hepatitis C virus (HCV) multiple antigens displaying bacteria's then turned it into ghost.The HCV core NS3 proteins' conserved epitopes were cloned AIDA gene plasmid auto transporter. The recombinant was transformed...
Gold nanoparticles (AuNPs) are commonly used in biosensors of various kinds. However, its application to extract DNA from cancer tissues has not been extensively studied. The purification is an important step diagnostic and therapeutic development. Almost, all cervical cases associated with high-risk human papillomavirus (HR-HPV) infection. Accurate viral diagnosis so far relied on the extraction adequate amounts formalin-fixed, paraffin-embedded (FFPE) tissue samples. Till now, no specific...
Aim: Human respiratory syncytial virus (HRSV) is a common cause of infections, particularly in infants and the elderly. Ribavirin only US FDA-approved antiviral drug for HRSV infection, but it has unwanted side effects. Methods: We engineered an shRNA targeting HRSV-M gene to antagonize replication. Results: The results showed that had similarly significant reduction viral load (99.8%) as ribavirin. In addition, combined treatment with ribavirin M-shRNA resulted compared or alone....
Background: Chemotherapy, the primary treatment for acute lymphoblastic leukemia (ALL), often yields inadequate responses. Epigallocatechin gallate (EGCG) has been shown to significantly affect tumor cells through various mechanisms, including cell cycle arrest, apoptosis, and autophagy. Objectives: The objective of this study is explore impact EGCG on autophagy, interplay between them in NALM-6, a pre-B-ALL line. Methods: NALM-6 were subjected concentrations 24 48 hours. Additionally, NH4Cl...
Background: There are few studies indicating the post-neonatal HBV vaccination level of anti-HBs antibody in first-year enrolled university students Iran. In addition, anti- HBc detection, which is a good indicator virus exposure, has not been reported vaccines. Hence, this study was conducted to determine and anti-HBc antibodies serum sample medical laboratory who had received primary infantile vaccination. Methods: This on department sciences at Shiraz University Medical Sciences, For...
Clonal eosinophilia is a hematologic disorder caused by translocation in growth factor receptor (GFR) genes. Despite the identified molecular mechanisms underlying clonal hypereosinophilia, distinction between and reactive has remained challenging due to diversity of partner genes for translocated GFRs. This study aimed examine feasibility phosphoflow cytometry diagnosis hypereosinophilia through evaluating level platelet-derived alpha (PDGFRA) phosphorylation its correlation with PDGFRA...