A5068897173- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Nanopore and Nanochannel Transport Studies
- RNA Interference and Gene Delivery
- Photosynthetic Processes and Mechanisms
- Endoplasmic Reticulum Stress and Disease
- Microtubule and mitosis dynamics
- Cellular Mechanics and Interactions
- Supramolecular Self-Assembly in Materials
- ATP Synthase and ATPases Research
- Nuclear Structure and Function
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
University of Denver
2017-2024
University of Washington
2017
Significance DNA helicases are enzymes that use energy from ATP hydrolysis to move along nucleic acid tracks and unwind double-stranded DNA. Helicases involved in every aspect of metabolism therefore vital maintaining genomic integrity. Using the single-molecule technique picometer-resolution nanopore tweezers (SPRNT), which measures position through biological membrane protein MspA as an enzyme moves DNA, we monitored kinetics helicase Hel308 at 1,000 times better temporal resolution than...
Large dense core vesicles (LDCVs) mediate the regulated release of neuropeptides and peptide hormones. They form at trans-Golgi network (TGN), where their soluble content aggregates to a core, but mechanisms controlling biogenesis are still not completely understood. Recent studies have implicated peripheral membrane protein HID-1 in neuropeptide sorting insulin secretion. Using CRISPR/Cas9, we generated KO rat neuroendocrine cells, show that absence results specific defects hormone...
The morphogenesis of developing tissues relies on extensive cellular rearrangements in shape, position, and identity. A key process reshaping is cell intercalation-driven elongation, where epithelial cells align intercalate along a common axis. Typically, analyses focus how peripheral cortical forces influence shape changes. Less attention given to inhomogeneities internal structures, particularly the nucleus, impact shaping. Here, we examine pulsed contractile extension dynamics interact...
Regulated secretion of neuropeptides and peptide hormones by secretory granules (SGs) is central to physiology. Formation SGs occurs at the trans-Golgi network (TGN) where their soluble cargo aggregates form a dense core, but mechanisms controlling sorting regulated cargoes (soluble transmembrane) away from constitutively secreted proteins remain unclear. Optimizing use retention using selective hooks method in (neuro-)endocrine cells, we now quantify TGN budding kinetics constitutive...
Abstract Regulated secretion of neuropeptides and peptide hormones by secretory granules (SGs) is central to physiology. Formation SGs occurs at the trans-Golgi network (TGN) where their soluble cargo aggregates form a dense core, but mechanisms controlling sorting regulated cargoes (soluble transmembrane) away from constitutively secreted proteins remain unclear. Optimizing use retention using selective hooks (RUSH) method in (neuro-)endocrine cells, we now quantify TGN budding kinetics...
Abstract Large dense core vesicles (LDCVs) mediate the regulated release of neuropeptides and peptide hormones. They form at trans -Golgi network (TGN) where their soluble content aggregates to a core, but mechanisms controlling biogenesis are still not completely understood. Recent studies have implicated peripheral membrane protein HID-1 in neuropeptide sorting insulin secretion. Using CRISPR/Cas9, we generated KO rat neuroendocrine cells, show that absence results specific defects hormone...