A novel extracellular thermo-alkali-stable laccase from Bacillus tequilensis SN4 (SN4LAC) was purified to homogeneity. The a monomeric protein of molecular weight 32 KDa. UV-visible spectrum and peptide mass fingerprinting results showed that SN4LAC is multicopper oxidase. Laccase active in broad range phenolic non-phenolic substrates. Catalytic efficiency (kcat/Km) 2, 6-dimethoxyphenol most efficiently oxidized by the enzyme. enzyme inhibited conventional inhibitors like sodium azide,...

Degradation of residual lignin in kraft pulp by chemical bleaching is implicated causing environmental pollution. The use thermo- and alkali-tolerant bacterial laccases considered to be important biological alternative processing. Laccases from Bacillus species have shown promise this respect but their intracellular/spore bound presence make industrial application economically unfeasible. We report here on a novel extracellular active thermo-alkali-stable laccase (SN4 laccase) which at 90 °C...

Laccases are multicopper oxidases containing four copper atoms per monomer distributed in three redox sites. Because of its tremendous applications different areas, isolation new laccases with wide range industrial implementation. The present study focuses on the optimization laccase production from Bacillus sp. MSK-01 under solid state fermentation conditions using fruit juice waste as substrate. MSKLAC was produced extracellularly by bacteria. This able to oxidize ABTS and syringaldazine....

In the past decade, a sudden increase in number and kind of emerging water contaminants has been observed. The can be categorized as organic or inorganic. Organic have known for years, techniques their detection remediation developed. However, inorganic pollutants are much more common. This is because they detected very low negligible concentrations equally toxic at higher concentrations. To boost research on pollutant contamination, advancements quantification required. presented paper...

A multicopper oxidase, laccases catalyze the four-electron reduction of substrate with use molecular oxygen. Laccases are abundant in nature and can be found virtually every form life on planet. Generally speaking, classified into three types: blue, white, yellow. Plant, bacterial fungal all have same trinuclear copper site for reduction. Non-phenolic as well phenolic molecules both capable being catalyzed by this enzyme. used a wide range industries that make chemicals. been subject recent...

Treatments of industrial effluent/s by enzymes offer significant advantage over the use microorganisms. However they have not been explored for bioremediation effluent from pulp and paper industry. This may be because less availability lignolytic which can active/stable under extreme environmental conditions that are present in treatment plant mills. In study thermoalkali stable laccase Bacillus tequilensis SN4 has mill effluent. Under standardized reduction various parameters , color 83%,...

Laccase in prokaryotes have enormous application as biocatalysts. Nevertheless, very few laccases been characterized structurally till date. Recently, a prokaryotic laccase from"Bacillus licheniformis NS2324" was cloned and expresses E. coli. The present research focused on silicostructure prediction characterization of gene isolated from "B. licheniformisNS2324".In this study various homology modeling servers were used to predict the protein structure laccase. Total 17 models predicted with...

?-Amylase has been in increasing demand industries due to its hydrolytic nature. Solid state fermentation (SSF) is a cost effective method for the enzyme production. In present study, amylase from Bacillus licheniformis MTCC 1483 was produced large quantity by solid using paddy straw as substrate. Response surface methodology useful tool optimizing many parameters at time and used 8523 IUg-1 of activity obtained under optimized conditions which lead 35 fold increase yield unoptimized condition.

Indigo carmine dye is used in many industries like textile, paper, cosmetics, etc. It specially textile for dyeing denim. The untreated water discharged from these industries, especially the industry, poses a significant environmental threat. conventional physicochemical methods treating industrial effluent are very expensive and cause secondary pollution. In present study, an extracellular laccase-producing Bacillus licheniformis NS2324 was isolated, crude enzyme to degrade indigo dye....

Malachite green, a triphenylmethane dye, is used in aquaculture to limit the growth of protozoans and fungi fish. It also employed food, medicinal, textile sectors. MG has high toxicity covering micro organisms higher eukaryotes. Its toxic effects include organ damage, developmental abnormalities mutagenic/carcinogenic potentials. Hence it utmost importance degrade MG. Therefore, this study with help catalytic properties laccase, biodegradation dye-malachite green was studied respect...