A5012091763- Bacterial biofilms and quorum sensing
- Antimicrobial Resistance in Staphylococcus
- Bacterial Genetics and Biotechnology
- Bacterial Identification and Susceptibility Testing
- Plant Virus Research Studies
- Bacterial Infections and Vaccines
- Antibiotic Use and Resistance
- Pneumonia and Respiratory Infections
- Mycobacterium research and diagnosis
- Microbial Metabolism and Applications
- RNA and protein synthesis mechanisms
- Oral microbiology and periodontitis research
- Genomics and Phylogenetic Studies
- Virology and Viral Diseases
- X-ray Diffraction in Crystallography
- Bacteriophages and microbial interactions
- Herpesvirus Infections and Treatments
- Streptococcal Infections and Treatments
- Antibiotic Resistance in Bacteria
- Poxvirus research and outbreaks
- Antimicrobial Peptides and Activities
- Antifungal resistance and susceptibility
- vaccines and immunoinformatics approaches
- Biochemical and Structural Characterization
- Molecular Biology Techniques and Applications
Universidade Federal do Rio de Janeiro
2009-2022
GlaxoSmithKline (Brazil)
2022
Instituto de Biofísica Carlos Chagas Filho
2009
Abstract Background Methicillin-resistant staphylococci can colonize and cause diseases in companion animals. Unfortunately, few molecular studies have been carried out Brazil other countries with the aim of characterizing these isolates. Consequently, little is known about potential role animals transmitting resistant bacteria to humans. In this work we searched for mecA gene among Staphylococcus isolates obtained from nasal microbiota 130 healthy dogs cats attended a veterinary clinic...
Staphylococcus aureus ica-independent biofilms are multifactorial in nature, and various bacterial proteins have been associated with biofilm development, including fibronectin-binding A B, protein A, surface SasG, proteases, some autolysins. The role of extracellular DNA (eDNA) has also demonstrated S. biofilms. Here, we constructed a Tn551 library, the screening identified two genes that affected formation, lrgB yycI. repressive effect both on development was confirmed knockout strains by...
The global spread of specific clones methicillin-resistant S. aureus (MRSA) has become a major public health problem, and understanding the dynamics geographical requires worldwide surveillance. Over past 20 years, ST239 lineage MRSA been recognized as an emerging clone across globe, with detailed studies focusing on isolates from Europe Asia. Less is known about this in South America, and, particularly, Brazil where it was predominant early 1990's to 2000's. To gain better introduction we...
Biofilm is considered an important virulence factor in nosocomial infections. Herein, we report the complete genome sequence of a variant methicillin-resistant Staphylococcus aureus, strain BMB9393, which highly disseminated Brazil. This belongs to lineage ST239 and displays increased ability accumulate ica-independent biofilm invade human epithelial cells.
Abstract Despite the existing antibiotics, antimicrobial resistance is a major challenge. Consequently, development of new drugs remains in great demand. Quinones part broad group molecules that present antibacterial activity besides other biological properties. The main purpose this study was to evaluate antibiofilm activities synthetic N , O -acetals derived from 2-amino-1,4-naphthoquinone [ 7a : 2-(methoxymethyl)-amino-1,4-naphthoquinone; 7b 2-(ethoxymethyl)-amino-1,4-naphthoquinone; and...
In this study, genotyping techniques including staphylococcal chromosomal cassette mec (SCCmec) typing, pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST) and restriction-modification tests were used to compare the molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) isolates recovered at two times within a 10-year interval (1998 2008) from tertiary Brazilian hospital. addition, antimicrobial susceptibility profiles analyzed. All 48 MRSA...
Biofilm formed by Staphylococcus aureus is considered an important virulence trait in the pathogenesis of infections associated with implantable medical devices. Gene expression analyses are strategies for determining mechanisms involved production and regulation biofilm. Obtaining intact RNA preparations first most critical step these studies. In this article, we describe optimized protocol obtaining total from sessile cells S. using RNeasy Mini Kit. This method essentially consists a few...
Cantagalo virus (CTGV) is the etiologic agent of a pustular disease in dairy cows and workers Brazil with important economical occupational impacts. Nevertheless, no antiviral therapy currently available. ST-246 potent inhibitor orthopoxvirus egress from cells has proved its efficacy cell culture animal models. In this work, we evaluated effect on CTGV replication. Plaque reduction assays indicated that 6-38 times more susceptible to drug than VACV-WR cowpox virus, respectively, an EC50...
Methicillin-resistant Staphylococcus aureus (MRSA) is still one of the most important hospital pathogen globally. The multiresistant isolates ST239-SCCmecIII lineage are spread over large geographic regions, colonizing and infecting patients in virtually all continents. balance between fitness (adaptability) virulence potential likely to represent an issue clonal shift dynamics leading success some specific MRSA clones another. accessory gene regulator (agr) master quorum sensing system...
Abstract Staphylococcus aureus is a versatile Gram-positive coccus frequently found colonizing the skin and nasal membranes of humans. The acquisition staphylococcal cassette chromosome mec was major milestone in evolutionary path methicillin-resistant S. . This genetic element carries mecA gene, main determinant methicillin resistance. MRSA involved plethora opportunistic infectious diseases. accessory gene regulator quorum sensing system, playing an important role virulence, including...
Abstract Background Antimicrobial resistance (AMR) is one of the biggest threats to global public health. Selection resistant bacteria driven by inappropriate use antibiotics, amongst other factors. COVID-19 may have exacerbated AMR due unnecessary antibiotic prescribing. Country-level knowledge needed understand options for action. Objectives To review situation with respect in Brazil and initiatives addressing it. Identifying areas where more information required will provide a call action...
Antimicrobial resistance (AMR) is one of the biggest threats to global public health. Selection resistant bacteria driven by inappropriate use antibiotics, amongst other factors. COVID-19 may have exacerbated AMR due unnecessary antibiotic prescribing. Country-level knowledge needed understand options for action.
Multidrug resistance is commonly acquired by transferring DNA from one bacterium to another. However, the mechanisms that enhance acquisitions of foreign genes are poorly understood, as well dynamics their transmission between hosts in different environments. Here, genomic approaches were applied evaluate enrichment S. aureus chromosome with traits groups genomes or without anti-restriction and analyze some evolutionary aspects these acquisitions. Furthermore, role played an gene improving...