A5074715165- Insect Resistance and Genetics
- Entomopathogenic Microorganisms in Pest Control
- Insect and Pesticide Research
- Insect symbiosis and bacterial influences
- Insect-Plant Interactions and Control
- Bacillus and Francisella bacterial research
- Plant Virus Research Studies
- Insect Utilization and Effects
Universitat de València
2014-2020
Instituto de Biomedicina de Valencia
2020
Instituto Valenciano de Investigaciones Agrarias
2012
Universitat Jaume I
2012
Significance Bacillus thuringiensis and its toxins are widely used for insect control. Notwithstanding the remarkable importance of this pathogen, killing mechanism has yet to be fully elucidated. Here we show that microbiota resident in host midgut triggers a lethal septicemia. The infection process is enhanced by reducing immune response control on replication bacteria invading body cavity through toxin-induced epithelial lesions. experimental approach used, leaving unaltered, allows...
Crops expressing genes from Bacillus thuringiensis (Bt crops) are among the most successful technologies developed for control of pests but evolution resistance to them remains a challenge. Insect resistant cotton and maize Bt Vip3Aa protein were recently commercialized, though not yet in Australia. We found that, although relatively high, frequency alleles field populations H. armigera Australia did increase over past four seasons until 2014/15. Three new isofemale lines determined be...
Vip3 proteins are secretable from Bacillus thuringiensis whose mode of action is still poorly understood. In this study, the activation process for was closely examined in order to better understand Vip3Aa protein stability and shed light on its structure. The protoxin (of 89 kDa) treated with trypsin at concentrations 1:100 120:100 (trypsin:Vip3A, w:w). If not properly neutralized, results SDS-PAGE analysis (as well as those Agrotis ipsilon midgut juice) equivocally indicated that could be...
Vip3 vegetative insecticidal proteins from Bacillus thuringiensis are an important tool for crop protection against caterpillar pests in IPM strategies. While there is wide consensus on their general mode of action, the details action not completely elucidated and structure remains unknown. In this work alanine scanning technique was performed 558 out total 788 amino acids Vip3Af1 protein. From residue substitutions, 19 impaired protein expression other substitutions severely compromised...