A5020958107- Insect Resistance and Genetics
- Insect and Pesticide Research
- Entomopathogenic Microorganisms in Pest Control
- Insect Pest Control Strategies
- Insect-Plant Interactions and Control
- Metabolism and Genetic Disorders
- Bacterial Infections and Vaccines
- Insect Utilization and Effects
- Polyamine Metabolism and Applications
- Invertebrate Immune Response Mechanisms
- Legume Nitrogen Fixing Symbiosis
- Insect symbiosis and bacterial influences
- Nematode management and characterization studies
- Escherichia coli research studies
- Genetically Modified Organisms Research
- Neurobiology and Insect Physiology Research
- Chromosomal and Genetic Variations
- Trypanosoma species research and implications
- Nicotinic Acetylcholine Receptors Study
- Lipid metabolism and biosynthesis
- Nanoparticles: synthesis and applications
- Enzyme Catalysis and Immobilization
- Antimicrobial Peptides and Activities
- Insect behavior and control techniques
- Date Palm Research Studies
Universitat de València
2015-2025
Instituto de Biomedicina de Valencia
2022
Bacillus thuringiensis Vip3 (Vegetative Insecticidal Protein 3) toxins are widely used in biotech crops to control Lepidopteran pests. These proteins produced as inactive protoxins that need be activated by midgut proteases trigger cell death. However, little is known about their three-dimensional organization and activation mechanism at the molecular level. Here, we have determined structures of protoxin protease-activated state Vip3Aa 2.9 Å using cryo-electron microscopy. The...
Current knowledge of biochemical mechanisms insect resistance to Bacillus thuringiensis is reviewed. Available information on inheritance and patterns cross-resistance included. Modification the binding sites for B. insecticidal crystal proteins has been found in different populations three species. This mechanism seems be inherited as a single recessive or partially major gene, levels reached are high. Altered proteolytic processing suggested involved one case resistance. From available...
Host-pathogen interactions result in complex relationship, many aspects of which are not completely understood. Vip proteins, Bacillus thuringensis (Bt) insecticidal toxins produced during the vegetative stage, selectively effective against specific insect pests. This new group Bt proteins represents an interesting alternative to classical Cry because current data suggests that they do share same mode action. We have designed and developed a genome-wide microarray for beet armyworm...
Vip3 proteins are secretable from Bacillus thuringiensis whose mode of action is still poorly understood. In this study, the activation process for was closely examined in order to better understand Vip3Aa protein stability and shed light on its structure. The protoxin (of 89 kDa) treated with trypsin at concentrations 1:100 120:100 (trypsin:Vip3A, w:w). If not properly neutralized, results SDS-PAGE analysis (as well as those Agrotis ipsilon midgut juice) equivocally indicated that could be...
The insecticidal Cry proteins from Bacillus thuringiensis are used in biopesticides or transgenic crops for pest control. Cry1I protein family has unique characteristics of being produced during the vegetative rather than sporulation phase, its protoxins forming dimers solution, and exhibiting dual toxicity against lepidopteran coleopteran pests. Cry1Ia protoxin undergoes sequential proteolysis N- C-terminal ends, producing intermediate forms with activity, while some cases, fully processed...
Anticarsia gemmatalis (velvetbean caterpillar) and Chrysodeixis includens (soybean looper, formerly named Pseudoplusia includens) are two important defoliating insects of soybeans. Both lepidopteran pests controlled mainly with synthetic insecticides. Alternative control strategies, such as biopesticides based on the Bacillus thuringiensis (Bt) toxins or transgenic plants expressing Bt toxins, can be used increasingly being adopted. Studies insect susceptibilities modes action different...
Antimicrobial peptides (AMPs) and lysozymes are the main effectors of insect immune system, they involved in both local systemic responses. Among responses, midgut reaction plays an important role fighting pathogens that reach body through oral route, as do many microorganisms used pest control. Under this point view, understanding how insects defend themselves locally during first phases infections caused by food-borne is to further improve microbial control strategies. In present study, we...
Abstract Vip3 proteins are produced by Bacillus thuringiensis and toxic against lepidopterans, reason why the vip3Aa gene has been introduced into cotton corn to control agricultural pests. Recently, structure of determined consists a tetramer where each monomer is composed five structural domains. The transition from protoxin trypsin‐activated form involves major conformational change N ‐terminal Domain I, which remodelled tetrameric coiled‐coil that thought insert apical membrane midgut...
The accurate determination of the number copies a gene in genome (gene dosage) is essential for genetic analyses. Quantitative real time PCR (qPCR) with TaqMan detection has shown advantages over traditional Southern-blot and FISH techniques, however high costs required labeled probes an important limitation this method. qPCR SYBR Green I simple inexpensive alternative, but it never been applied to copy low genes organisms allelic variability (as some insects), where very small margin error...
Bacillus thuringiensis (Bt) produces insecticidal proteins that are either secreted during the vegetative growth phase or accumulated in crystal inclusions (Cry proteins) stationary phase. Cry1I share three domain (3D) structure typical of but to media early In generally accepted mode action 3D Cry (sequential binding model), formation an oligomer (tetramer) has been described as a major step, necessary for pore and subsequent toxicity. To know if this could be extended proteins, Cry1Ia...