A5030687023- Chromosomal and Genetic Variations
- Genetic Neurodegenerative Diseases
- Insect Resistance and Genetics
- Mitochondrial Function and Pathology
- Entomopathogenic Microorganisms in Pest Control
- Plant Disease Resistance and Genetics
- Plant Virus Research Studies
- Genetic diversity and population structure
- Genomics and Phylogenetic Studies
- Insect and Arachnid Ecology and Behavior
- Insect Pest Control Strategies
- Insect and Pesticide Research
- Evolution and Genetic Dynamics
- Neurobiology and Insect Physiology Research
- Silkworms and Sericulture Research
- Studies on Chitinases and Chitosanases
- DNA Repair Mechanisms
- Heat shock proteins research
- Autophagy in Disease and Therapy
- Insect symbiosis and bacterial influences
- Insect behavior and control techniques
- thermodynamics and calorimetric analyses
- Ubiquitin and proteasome pathways
- Endoplasmic Reticulum Stress and Disease
- Physiological and biochemical adaptations
Universitat de València
1997-2018
Friedreich ataxia (FA), the most common form of hereditary ataxia, is caused by a deficit in mitochondrial protein frataxin. While several hypotheses have been suggested, frataxin function not well understood. Oxidative stress has suggested to play role pathophysiology FA, but this view recently questioned, and its link unclear. Here, we report use RNA interference (RNAi) suppress Drosophila gene (fh) expression. This model system parallels situation FA patients, namely moderate systemic...
Friedreich's ataxia (FRDA), the most common inherited in Caucasian population, is a multisystemic disease caused by significant decrease frataxin level. To identify genes capable of modifying severity symptoms depletion, we performed candidate genetic screen Drosophila RNAi-based model FRDA. We found that reduction TOR Complex 1 (TORC1) signalling improves impaired motor performance phenotype FRDA flies. Pharmacologic inhibition TORC1 rapamycin also restored this and increased lifespan ATP...
Several P homologous sequences have been cloned and sequenced from Drosophila subobscura. These are located at the 85DE region of O chromosome least three them organized in tandem. We identified four copies which exhibit strong similarity between them. All Isolated elements truncated 5′ 3′ ends. They lost inverted terminal repeats exon 3, but maintain exons 0, 1 2. transcribed producing a polyadenylated RNA. The structure these transcripts suggests that they able to encode 66 kd...
Friedreich's ataxia (FA), the most frequent form of inherited ataxias in Caucasian population, is caused by a reduced expression frataxin, highly conserved protein. Model organisms have contributed greatly efforts to decipher function frataxin; however, precise this protein remains elusive. Overexpression studies are useful approach investigate mechanistic actions existing literature reports contradictory results. To further effect frataxin overexpression, we analyzed consequences...
Friedreich's ataxia (FRDA), the most commonly inherited in populations of European origin, is a neurodegenerative disorder caused by decrease frataxin levels. One hallmarks disease accumulation iron several tissues including brain, and has been proposed to play key role homeostasis. We found that levels zinc, copper, manganese aluminum were also increased Drosophila model FRDA, copper zinc chelation improve their impaired motor performance. By means candidate genetic screen, we identified...
Sequences homologous to the gypsy retroelement from Drosophila melanogaster are widely distributed among drosophilids. The structure of includes an open reading frame resembling retroviral gene env, which is responsible for infectious properties retroviruses.In this study we report molecular and phylogeny analysis complete env ten species obscura group genus one Scaptomyza.The results indicate that in most cases sequences could produce a functional Env protein therefore maintain capability...
The Ty3/gypsy family of retroelements is closely related to retroviruses, and some their members have an open reading frame resembling the retroviral gene env. Sequences homologous gypsy element from Drosophila melanogaster are widely distributed among species. In this work, we report a phylogenetic study based mainly on analysis 5′ region env several species obscura group, also sequences already reported D. melanogaster, virilis, hydei. Our results indicate that elements group constitute...
Heat shock response was investigated in three species of the obscura group Drosophila genus (D. subobscura, D. guanche, and madeirensis) by chromosome cytology analysis [ 3 H]uridine labeling. A set eight puffs (2C, 15DE, 18C, 27A, 31CD, 85AB, 89A, 94A) were induced after heat treatments each species; 94A most heavily labeled autoradiograms conditions. From situ results using major genes melanogaster as a probe, it inferred that 94A, 27A loci are homologous to loci, which contain, HSP82,...
We used the repetitive character of transposable elements to isolate a non-LTR retrotransposon in Drosophila subobscura. bilbo, as we have called it, has homology TRIM and LOA elements. Sequence analysis showed 5' untranslated region (UTR), an open reading frame (ORF) with no RNA-binding domains, downstream ORF that had structural I factor, and, finally, 3' UTR which ended several 5-nt repeats. The results our phylogenetic analyses shed light on evolution retrotransposons support hypothesis...
The P element homologous sequences of the two closely related species Drosophila guanche and subobscura represent a very special case transposable-element derivatives. Although they have lost regions known to be essential for transposition by random mutations, all them selectively conserved coding capacity "P-repressor-like" proteins during past few millions years. In both species, are tandemly amplified in single euchromatic gene cluster at equivalent chromosomal positions. contrast,...
The bilbo element is a non-LTR retrotransposon isolated from Drosophila subobscura. We conducted distribution survey by Southern blot for 52 species of the family Drosophilidae, mainly obscura and melanogaster groups. Most analyzed bear sequences homologous to D. In group, same subgroup also share similar patterns. To investigate phylogenetic relationship among these elements, we eight copies short sequence several group. obtained phylogram agrees with phylogeny species, which suggests...