The significant increase in life expectancy is closely related to the growing interest impact of aging on function and appearance skin. Skin influenced by several factors, solar ultraviolet (UV) irradiation considered one most important causes skin photoaging. aim this study was examine anti-photoaging role porphyra-334 from Porphyra (P.) yezoensis, a mycosporine-like amino acid (MAA), using high-performance liquid chromatography (HPLC), electrospray ionization‑mass spectrometry (ESI-MS). In...

A mesoporous silica-based drug delivery system (MS@PNIPAm-PAAm NPs) was synthesized by conjugating the PNIPAm-PAAm copolymer onto silica (MS) surface as a gatekeeper that responds to temperature and pH changes. The studies are carried out in vitro at different (7.4, 6.5, 5.0) temperatures (such 25 °C 42 °C, respectively). conjugated (PNIPAm-PAAm) acts below lower critical solution (LCST) (<32 °C) collapsed globule structure above LCST (>32 °C), resulting controlled from MS@PNIPAm-PAAm...

Insulin-like growth factor-binding protein-2 and -3 (IGFBP-2 -3) are members of a family proteins that present in extracellular fluids bind IGF-I -II. IGFBP-2 is regulated differently from IGFBP-3, because its serum concentrations elevated some adults with GH deficiency (GHD), whereas IGFBP-3 usually decreased. The purposes this study were to define the normal range children, determine efficacy diagnosis GHD, compare diagnostic value measurements response provocative testing results IGFBP-2,...

Rheumatoid arthritis (RA) is a chronic inflammatory disease that characterized by hyperplasia of the synovial fibroblasts, which partly result decreased apoptosis. This study investigated mechanisms through curcumin, polyphenolic compound from rhizome Curcuma longa, exerts its anti-proliferative action in fibroblasts obtained patients with RA. Exposure to curcumin resulted growth inhibition and induction apoptosis, as measured MTT assay, fluorescent microscopy Annexin-V-based assay. RT-PCR...

Because seaweed extracts have recently been found to antioxidant and anti-tumor activities, we analyzed a hot-water-soluble polysaccharide (PS) of the marine alga Capsosiphon fulvescens for its potential as functional foodstuff by determining effects on cell growth DNA synthesis. MTS assays showed that C. PS (Cf-PS) significantly inhibited proliferation cultured human cancer cells in dose-dependent manner. Cf-PS-treated AGS exhibited marked increase caspase-3 activation decrease Bcl-2...

Abstract: Fucoidan, a sulfated polysaccharide purified from brown algae, possesses variety of pharmacologic effects, including antiinflammatory, antioxidant, and anticancer properties; however, the underlying action mechanisms are not completely understood. This study investigated possible through which fucoidan exerts its antiproliferative in cultured AGS human gastric adenocarcinoma cells. We found that effectively inhibits growth cells by inducing autophagy, as well apoptosis. Apoptosis...

Laminarin, found in marine brown algae, is used as a carbohydrate reserve for phytoplankton; however, it also traditional Chinese medicine, and has been shown to have several biological activities, including anticancer activities. In this study, we examined the mechanisms through which laminarin from Laminaria digitata induces apoptosis HT-29 colon cancer cells, well involvement of ErbB signaling pathway. Cell viability assay revealed that induced cell death dose-dependent manner. cycle...

Pyropia yezoensis (P. yezoensis) is an important marine algae. Its high protein content serves as a good source of biologically active peptides. Potent inhibitory effects on the production inflammatory mediators were observed in bioactive peptide derived from P. (peptide yezoensis; PPY1), demonstrated lipopolysaccharide (LPS)‑stimulated macrophages. The present study showed that concentrations ranging 250 to 1,000 ng/ml had no significant cytotoxicity cell viability assay when applied RAW...

Insulin-like growth factor (IGF)-binding protein-5 (IGFBP-5) has been shown to bind extracellular matrix (ECM) with relatively high affinity, but the ECM components that mediate this interaction have not identified. These studies show radiolabeled IGFBP-5 specifically coprecipitates two proteins, thrombospondin-1 (TSP-1) and osteopontin (OPN). As TSP-1 binds avidly heparin, as does IGFBP-5, effect of glycosaminoglycans on TSP-1/IGFBP-5 was analyzed. Heparan dermatan sulfate inhibited...

In recent years, algae have been highlighted as potential sources of anticancer agents. Laminarin is a molecule found in marine brown that has potentially beneficial biological activities. However, these activities not investigated. the present study, we examined effects laminarin on HT-29 cells and analyzed its effect insulin-like growth factor (IGF-IR) signaling pathway. 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxy-phenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assays revealed induced...

Macrophage polarization has been well documented. Macrophages can aquire two phenotypes, the pro-inflammatory M1 phenotype, and anti-inflammatory wound healing M2 phenotype. The macrophage phenotype linked to metabolic disease is also associated with cancer-related inflammation. Of note, be influenced by extracellular environment. In current study, we examined effects of Pyropia yezoensis glycoprotein (PYGP) on in lipopolysaccharide (LPS)-stimulated macrophages. RAW 264.7 macrophages...

The objective of the present study was to identify peptides, based on active components red algae seaweed Pyropia yezoensis, able inhibit generation reactive oxygen species (ROS), which is associated with aging and oxidative activities. Phycobilin, specific algae, covalently binds water‑soluble proteins. There are three types pigment bound proteins, known as phycobiliproteins (PBPs): Phycoerythrin (PE), phycocyanin (PC) allophycocyanin (APC). In study, PBPs reported previously have...

Modulation of multiple protein targets with a single compound is essential for the effective treatment central nervous system disorders. In our previous G protein-coupled receptor (GPCR) cell-based study, selective human monoamine oxidase (hMAO)-A inhibitor, eckol, stimulated activity dopamine D3 and D4 receptors. This result led to interest in marine phlorotannin-mediated modulation hMAO enzymes related GPCRs neuronal Here, we evaluate multi-target effects phloroglucinol,...

Phycoerythrin is a major light-harvesting pigment of red algae and cyanobacteria that widely used as fluorescent probe or colorant in the food cosmetic industries. In this study, phycoerythrin was extracted from Pyropia yezoensis purified by ammonium sulfate precipitation various chromatography methods. The analyzed UV-visible fluorescence spectroscopy. isolated had typical spectrum R-phycoerythrin, with trimmer state absorbance maxima at 497, 536, 565 nm. It further identified LC-MS/MS...

Cultured fibroblasts secrete an 88-kDa serine protease that cleaves insulin-like growth factor binding protein-5 (IGFBP-5). Because IGFBP-5 has been shown to regulate IGF-I actions, understanding the chemical identity and regulation of this is important for how stimulates anabolic functions. The was purified from human fibroblast-conditioned medium by hydrophobic interaction, lectin affinity, heparin Sepharose affinity chromatography followed SDS-polyacrylamide gel electrophoresis. An band...