A5102920659- Muscle Physiology and Disorders
- Phosphodiesterase function and regulation
- Enzyme function and inhibition
- Protein Kinase Regulation and GTPase Signaling
- Children's Physical and Motor Development
- Receptor Mechanisms and Signaling
- Cholinesterase and Neurodegenerative Diseases
- Ion channel regulation and function
- Cardiomyopathy and Myosin Studies
- Sulfur-Based Synthesis Techniques
- Phytochemicals and Antioxidant Activities
- Muscle activation and electromyography studies
- Metabolism, Diabetes, and Cancer
- Neuroscience and Neuropharmacology Research
- Glycosylation and Glycoproteins Research
- Force Microscopy Techniques and Applications
- Hemoglobin structure and function
- Metal complexes synthesis and properties
- Renin-Angiotensin System Studies
- Neuropeptides and Animal Physiology
- Neurogenetic and Muscular Disorders Research
- Trace Elements in Health
- Environmental Toxicology and Ecotoxicology
- Advanced Sensor and Energy Harvesting Materials
- Prosthetics and Rehabilitation Robotics
Vanderbilt University Medical Center
2020-2025
University of California, San Francisco
1991
University of Nebraska Medical Center
1976-1987
Emory University
1976
Yale University
1971-1973
Abstract An assay method has been developed for the measurement of tissue levels guanosine 3',5'-monophosphate (cyclic GMP) based upon ability cyclic nucleotide to activate GMP-dependent protein kinase prepared from either lobster tail muscle or silkmoth pupal fat body. The used measure GMP in various biological tissues and human urine, slices heart brain incubated under conditions. results obtained with this new agree well published values GMP. limit sensitivity is about 0.5 pmole an...
Abstract A heat-stable protein which modulates the activity of cyclic nucleotide-dependent kinases was purified from lobster tail muscle, and its properties were studied. The modulator is similar in several respects to inhibitory factor reported by others (Appleman et al. (1969) Arch. Biochem. Biophys. 116, 39; Walsh (1971) J. Biol. Chem. 246, 1977) be present rat rabbit skeletal muscle. Polyacrylamide gel electrophoresis suggested presence aggregate forms modulator, each possessed both...
Guanosine 3':5'-monophosphate (cyclic GMP)-dependent protein kinase was purified from the guinea pig fetal lung, a tissue shown to be richest in this enzyme all mammalian sources examined, and its general properties studied. The 150-fold crude extract by steps of pH 5.4 isoelectric precipitation, Sephadex G-200 filtration, hydroxylapatite treatment DEAE-cellulose chromatography. enzyme, free contamination with adenosine AMP)-dependent kinase, had specific activity at least equivalent...
Abstract Several factors were found which determine whether the effect of modulator on protein kinase activity is stimulatory or inhibitory in nature. These include kind and concentration substrate protein, divalent metal ions, cyclic nucleotide, source modulator, nature enzyme. The effects guanosine 3',5'-monophosphate-dependent adenosine holoenzymes reproduced by using isolated catalytic subunits prepared from respective holoenzymes.
Abstract Background: Cardiomyopathy is the leading cause of death in patients with Duchenne muscular dystrophy. The relationship between cardiac and skeletal muscle progression unclear. objective this study was to evaluate correlation activity cardiomyopathy. We hypothesised that cardiomyopathy are directly related. Methods: Physical assessed accelerometers worn for 7 days. Average (vector magnitude/min) percentage time different activities were reported. Cardiac MRI used assess left...
Background: Physical activity, assessed by accelerometers, has been proposed as a quantitative outcome measure for patients with DMD, but research is limited Objective: To assess the total amount and patterns of physical activity in DMD using accelerometers.Methods: was (n = 49, 13.6 ± 4.0-year-old) age-and sex-matched healthy controls 15, 14.0 2.3-year-old) wrist-and ankle-worn accelerometers.To accelerometer recordings were converted into acceleration estimates (counts/min).Patterns time...
The current study tested the concept that cine magnetic resonance (MR) imaging and phosphorus-31 MR spectroscopy might be used to provide a comprehensive evaluation of functional metabolic status myocardium in humans. Thirteen patients with congestive cardiomyopathy eight healthy volunteers were imaged at 1.5 T one-dimensional chemical shift technique for localization P-31 an electrocardiographically referenced gradient refocused sequence heart. Prominent peaks PDE PME regions observed...
We have investigated the effects of various tumor promoting agents on protein kinase C activity in adult female SENCAR mice. Topical application benzoyl peroxide increased calcium-independent particulate fraction basal epidermal cells while decreasing soluble isolated from Benzoyl apparently altered indirectly, as it had no direct effect partially purified bovine brain. 12- O -tetradecanoyl phorbol-13-acetate to mouse skin resulted associated with change calcium-dependence enzyme. Since...
To adapt and validate a previously developed decision tree for youth to identify bedrest use in preschool children.Parents of healthy (3-6-year-old) children (n = 610; 294 males) were asked help them wear an accelerometer 7 10 days 24 hours/day on their waist. Children with ≥3 nights valid recordings randomly allocated the development 200) validation groups. Wear periods from identified minute-by-minute as or wake using visual identification by two independent raters. automate...
ABSTRACT Introduction/Aims Skeletal muscle magnetic resonance imaging (MRI) is a validated noninvasive tool to assess Duchenne muscular dystrophy (DMD) progression. There interest in finding DMD biomarkers that decrease the burden of clinical trial participation, such as wearable devices. Our aim was evaluate relationship between activity, via accelerometry, and skeletal MRI, particularly T 2 mapping. Methods children young adults completed MRI were asked wear an accelerometer on dominant...
Journal Article Effect of withdrawal from chronic propranolol treatment on high-affinity choline uptake in rat brain Get access L Charles Murrin, Murrin Department Pharmacology, University Nebraska Medical Center, 42nd and Dewey Avenue, Omaha, NE 68104, U.S.A. Correspondence. Search for other works by this author on: Oxford Academic Google Scholar Richard H Kennedy, Kennedy Thomas E Donnelly Pharmacy Volume 35, Issue 10, October 1983, Pages 677–679,...