A5089559945- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- Bacterial Genetics and Biotechnology
- Amoebic Infections and Treatments
- Radiopharmaceutical Chemistry and Applications
- Glycosylation and Glycoproteins Research
- Bacteriophages and microbial interactions
- Trace Elements in Health
- Escherichia coli research studies
- Lipid Membrane Structure and Behavior
- Probiotics and Fermented Foods
- Monoclonal and Polyclonal Antibodies Research
- Antibiotic Resistance in Bacteria
- RNA and protein synthesis mechanisms
- Neuroscience of respiration and sleep
- Iron Metabolism and Disorders
- Yersinia bacterium, plague, ectoparasites research
- Microbial infections and disease research
- Antimicrobial Resistance in Staphylococcus
- Inhalation and Respiratory Drug Delivery
- Radioactive element chemistry and processing
- Streptococcal Infections and Treatments
- Influenza Virus Research Studies
- Genomics and Phylogenetic Studies
- Enterobacteriaceae and Cronobacter Research
Universidade de Santiago de Compostela
2005-2017
Health Protection Agency
2009
Universidade da Coruña
1986
The 37-kDa ferric binding protein, FbpA, from three Neisseria meningitidis strains was purified to homogeneity with iron-affinity chromatography and used for immunisation of mice employing four different adjuvants: aluminium hydroxide, Freund's, the saponin Quil-A, a Ribi adjuvant system (RAS). Controls immunised without were also included. All sera obtained monospecific meningococcal antibody titres higher when RAS Quil-A (256), PBS resulting in similar those Freund's (64), and,...
Growing Neisseria meningitidis on an iron restriction medium induces the synthesis of new outer membrane proteins, some them true iron-regulated proteins (IROMPs) and others synthesized because stress produced by restriction. Some these are antigenic can be considered for development vaccines; this is especially desirable in case N. serogroup B, which polysaccharide vaccines not efficient. The antigenicity 37- 70-kDa IROMPs has been studied previously; work, we immunogenicity heterogeneity...
Two-dimensional diagonal SDS-PAGE was used to resolve membrane complexes and identify proteins with temperature-dependent mobility in Neisseria meningitidis N. lactamica. The main were composed of porins formed by heteromers PorA, PorB RmpM meningitidis, Also, other proteins, including Opa, clearly demonstrated. method allows improved detection the components which is difficult analytical approaches. Keywords: • lactamica shift electrophoresis
Abstract The structure of the porin complexes Neisseria meningitidis was assessed in vaccine strain H44/76 and its homologous mutants lacking main porins (PorA PorB) other outer membrane (OM) components (RmpM FetA). analysis using 1‐D blue native (BN) electrophoresis, 2‐D BN/SDS‐PAGE diagonal followed by LC/MS‐MS (for gels) or MALDI‐TOF revealed at least six wild‐type with molecular masses (MW) ranging from 145 to 195 kDa variable composition: two higher MW are formed PorA, PorB RmpM,...
The identification and characterization of meningococcal outer membrane vesicle complexes can be important for gaining an in-depth understaining their structure functionality. Analysis the complexome by 'traditional' 2-D analysis, in which isoelectrofocusing is used separation first dimension, hampered high hydrophobicity extreme isoelectric points many relevant proteins. using Blue Native (nondenaturing) electrophoresis instead dimension showed several porin complexes, but composition could...
Psychrotrophic bacteria isolated from raw milk were tested for their ability to adhere steel, two types of rubber, and glass, materials employed in the construction milking equipment. The adherence assays carried out by exposure radioactively labelled both a buffering solution (Ringer's) milk. degree Gram-positive was lower (P less than 0.001) that Gram-negative bacteria. Glass material least prone bacterial 0.001); there no significant differences between other three materials. Milk found...
The relative surface charge and free energy of forty-one coagulase-negative staphylococci were found to be normally distributed; therefore, they can considered a homogeneous group under strict statistical criteria. adherence these bacteria eight different biomaterials (seven synthetic one biologic) was independent variations in during adhesion. Adherence explained as thermodynamic process (free decreased with adherence), except the case bovine pericardium which increases. With biomaterials,...
The meningococcal NMB0035 locus encodes a 47 kDa outer-membrane protein that is highly conserved antigenically, and able to induce antibodies during infection bactericidal responses in vitro. This study analysed the surface exposure of this using specific flow cytometry assays determined its nucleotide sequence 33 Neisseria strains. Genomic analyses revealed no significant differences or amino acid sequences, but showed accessibility was variable among These results suggest masking by and/or...
Iron uptake analysis suggested that the Neisseria meningitidis transferrin (Tf) binding proteins, TbpA and TbpB, form only one type of receptor complex. Mutants defective in synthesis either or but not both can bind Tf, suggesting proteins are surface exposed function Tf binding. Also, iron from into meningococci did require presence Tbps. The TbpB-defective mutant incorporated c. 37% taken up by wild-type strain, this was insufficient for bacterial growth. TbpA-defective 50% strain able to...
A total of 103 Neisseria strains, including 42 carrier and 40 invasive N. meningitidis 21 commensal species were studied. Outer-membrane proteins from showed similar molecular mass distributions (except in the range 78 to 82 kDa which 90% but only 47.6% strains expressed proteins), many showing neither class II (41 kDa) nor III (38 proteins. When grown iron-restricted conditions induced mainly ranges 62 92 with no significant differences between groups. Commensal species, both normal...
Three recombinant proteins of Neisseria meningitidis, rPorB, rPorA, and rRmpM, were purified incorporated into liposomes prepared by dialysis-extrusion. The protein complexes formed using different combinations studied high resolution clear native electrophoresis (hrCNE) 2-D hrCNE/SDS-PAGE, analyzing the influence stoichiometry two porins in formation comparing them with porin present OMVs from five N. meningitidis strains. Insertion allowed a complete refolding complexes, electrophoretic...