A5031992145- Bacterial Infections and Vaccines
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Pneumonia and Respiratory Infections
- Bacterial biofilms and quorum sensing
- Lipid Membrane Structure and Behavior
- Protist diversity and phylogeny
- Escherichia coli research studies
- Vibrio bacteria research studies
- Monoclonal and Polyclonal Antibodies Research
- Protein Structure and Dynamics
- Advanced Drug Delivery Systems
- Influenza Virus Research Studies
- Reproductive tract infections research
- Streptococcal Infections and Treatments
- Bacterial Identification and Susceptibility Testing
- Microbial Community Ecology and Physiology
- Infective Endocarditis Diagnosis and Management
- Amoebic Infections and Treatments
- Yersinia bacterium, plague, ectoparasites research
- Diffusion and Search Dynamics
- Genomics and Phylogenetic Studies
- CAR-T cell therapy research
- Heat shock proteins research
- Polyomavirus and related diseases
Universidade de Santiago de Compostela
2012-2025
Sanford Consortium for Regenerative Medicine
2024
Tufts University
2022-2023
Indiana University Bloomington
2017-2022
Universidad de Navarra
2021
Indiana University
2019
Instituto Politécnico Nacional
2016
Antimicrobial resistance is a worldwide problem that urges novel alternatives to treat infections. In attempts find molecules, we assess the antimicrobial potential of seven essential oils (EO) different plants (Pinus sylvestris, Citrus limon, Origanum vulgare, Cymbopogon martini, Cinnamomum cassia, Melaleuca alternifolia and Eucalyptus globulus) against two multidrug-resistant bacteria species, i.e. Neisseria gonorrhoeae Streptococcus suis. EOs P. sylvestris C. limon revealed higher...
Bacterial swimming in confined two-dimensional environments is ubiquitous nature and clinical settings. Characterizing individual interactions between bacteria 2D confinement will help to understand diverse microbial processes, such as bacterial swarming biofilm formation. Here we report a novel motion pattern displayed by flagellated confinement: When two nearby cells align their moving directions, they tend engage cohesive without direct cell body contact, result of hydrodynamic...
Tuberculosis (TB) is a life-threatening disease and main cause of death worldwide. It mainly affects the lungs, it attributed to infection with Mycobacterium tuberculosis (MTB). Current treatments consist oral administration combinations antibiotics including rifabutin, in high doses for long periods time. These therapeutic regimens are associated many side effects rates drug resistance. To overcome these problems, this study aims at developing nanosystem improved delivery antibiotics,...
Two-dimensional diagonal SDS-PAGE was used to resolve membrane complexes and identify proteins with temperature-dependent mobility in Neisseria meningitidis N. lactamica. The main were composed of porins formed by heteromers PorA, PorB RmpM meningitidis, Also, other proteins, including Opa, clearly demonstrated. method allows improved detection the components which is difficult analytical approaches. Keywords: • lactamica shift electrophoresis
Bacillus subtilis exhibits swarming motility, a flagellar-mediated form of surface motility. Here, we use transposon mutagenesis and sequencing (TnSeq) to perform high-throughput screen for candidate genes required swarming. The TnSeq approach identified all the known flagellar biosynthesis nearly previously reported regulators that promote Moreover, an additional 36 improve validated them individually. Among these, two mutants with severe defects were recovered, including fliT,...
Abstract The structure of the porin complexes Neisseria meningitidis was assessed in vaccine strain H44/76 and its homologous mutants lacking main porins (PorA PorB) other outer membrane (OM) components (RmpM FetA). analysis using 1‐D blue native (BN) electrophoresis, 2‐D BN/SDS‐PAGE diagonal followed by LC/MS‐MS (for gels) or MALDI‐TOF revealed at least six wild‐type with molecular masses (MW) ranging from 145 to 195 kDa variable composition: two higher MW are formed PorA, PorB RmpM,...
Single-cell proteomics has emerged as a powerful technology for unraveling the complexities of cellular heterogeneity, enabling insights into individual cell functions and pathologies. One primary challenges in single-cell is data generation, where low mass spectral signals often preclude triggering MS2 events. This challenge addressed by Data Independent Acquisition (DIA), acquisition strategy that does not depend on peptide ion isotopic signatures to generate an event. In this study, we...
The identification and characterization of meningococcal outer membrane vesicle complexes can be important for gaining an in-depth understaining their structure functionality. Analysis the complexome by 'traditional' 2-D analysis, in which isoelectrofocusing is used separation first dimension, hampered high hydrophobicity extreme isoelectric points many relevant proteins. using Blue Native (nondenaturing) electrophoresis instead dimension showed several porin complexes, but composition could...
It was in the 1800s when first public publications about infection and treatment of gonorrhoea were released. However, prevention programmes only published a hundred years later. In 1940s, concept vaccination introduced into clinical to address early sulphonamide resistance. Since then, tons on
Random transposon mutagenesis is a powerful and unbiased genetic approach to answer fundamental biological questions. Here, we introduce an improved
The meningococcal NMB0035 locus encodes a 47 kDa outer-membrane protein that is highly conserved antigenically, and able to induce antibodies during infection bactericidal responses in vitro. This study analysed the surface exposure of this using specific flow cytometry assays determined its nucleotide sequence 33 Neisseria strains. Genomic analyses revealed no significant differences or amino acid sequences, but showed accessibility was variable among These results suggest masking by and/or...
Three recombinant proteins of Neisseria meningitidis, rPorB, rPorA, and rRmpM, were purified incorporated into liposomes prepared by dialysis-extrusion. The protein complexes formed using different combinations studied high resolution clear native electrophoresis (hrCNE) 2-D hrCNE/SDS-PAGE, analyzing the influence stoichiometry two porins in formation comparing them with porin present OMVs from five N. meningitidis strains. Insertion allowed a complete refolding complexes, electrophoretic...