A5102600791- Microtubule and mitosis dynamics
- Autophagy in Disease and Therapy
- Intraperitoneal and Appendiceal Malignancies
- Endometriosis Research and Treatment
- Ovarian cancer diagnosis and treatment
- Connective tissue disorders research
- Genetic factors in colorectal cancer
- Cancer Genomics and Diagnostics
- Atmospheric Ozone and Climate
- Atmospheric aerosols and clouds
- Genetics and Neurodevelopmental Disorders
- MicroRNA in disease regulation
- Cardiac Valve Diseases and Treatments
- Extracellular vesicles in disease
- Aortic Disease and Treatment Approaches
- Cellular transport and secretion
- Aortic aneurysm repair treatments
- Prostate Cancer Treatment and Research
- Atmospheric chemistry and aerosols
The University of Texas MD Anderson Cancer Center
2025
Peking University
2024
State Key Joint Laboratory of Environment Simulation and Pollution Control
2024
Nanjing University of Information Science and Technology
2024
Jinhua Central Hospital
2024
State Key Laboratory of Biotherapy
2023
Fudan University
2023
Sichuan University
2023
Abstract Treatment decisions in metastatic castration-resistant prostate cancer (mCRPC) are mostly guided by clinical variables, but efforts to molecularly monitor the disease remain hampered challenges acquiring tumor tissue repeatedly. Here, we simultaneously profiled genome copy number and exome longitudinal plasma circulating DNA (ctDNA) acquired before, during, upon progression serial treatments with androgen signaling inhibitors (ASI) taxane chemotherapy from 60 mCRPC patients (2-10...
Antibodies targeting folate receptor 1 (FOLR1) induce autophagic cell death in addition to antibody-dependent cytotoxicity, but the biological relevance of anti-FOLR1 antibody-induced autophagy for clinical applications remains unclear. Here, we investigated role triggered by IMGN853 (mirvetuximab soravtansine), a FOLR1-targeted antibody-drug conjugate, and explored potential combinations with chemotherapeutic drugs used ovarian cancer treatment. We discovered that FOLR1 was predominantly...
<p>Time-lapse videos of GFP and RFP fluorescence in ptLC3-pEGFP-RFP–expressing OVCAR-8 cells during treatment with IMGN853 (Movie 2)</p>
<p>IMGN853 induces beclin-1–dependent autophagic cell death in FOLR1<sup>+</sup> HGSC lines. <b>A,</b> Percentages of vacuoles OVCAR-8 and OVCA432 cells FOLR1<sup>low</sup>A2780 treated with control (formulation buffer), IMGN853 alone, HCQ or IMGN853+HCQ for 48 hours analyzed by acridine orange staining followed FACS. <i>n</i> = 6; error bars represent SEMs. <b>B,</b> SYTOX<sup>+</sup> dead among...
<p>Combination therapy with IMGN853 and the anti–VEGF-A antibody B20 in OVCAR-8 model. <b>A,</b> Schematic representation of mouse models established using ip2-luc cells (4 × 10<sup>6</sup>/mouse). <b>B,</b> Twenty-one days after cell injection as shown by bioluminescence signals representative mice from IVIS imaging, were randomized to receive buffer (control), alone (5.2 mg/kg, once weekly), (5 twice weekly, intraperitoneally), or IMGN853+B20....
<p>Time-lapse videos of GFP and RFP fluorescence in ptLC3-pEGFP-RFP–expressing OVCAR-8 cells during treatment with the control formulation buffer (Movie 1)</p>
<p>Effects of IMGN853 on the survival and inhibition tumor growth mice bearing ovarian high-grade serous carcinoma (HGSC) PDX tumors. <b>A,</b> Main clinical pathologic characteristics tissues from patients with HGSC. Date Implantation (DATE) <b>B,</b> Schematic representation experiments. mouse models were established by surgical implantation HGSC tumors via intraovarian injection. Treatment either formulation buffer (control) or (5.2 mg/kg, once weekly) was...
<p>Combination therapy with IMGN853 and topotecan in the OVCAR-8 model. <b>A,</b> Results of MTT assays showing combinatorial effects different concentrations on viability cells after 72 hours treatment [shown as log IMGN853(M)]. <i>n</i> = 3; data are mean ± SD. <b>B,</b> Synergy quantification drug combination using Chou–Talalay analysis was reflected by CI plot for topotecan. <b>C,</b> Schematic representation mouse models ovarian...
<p>IMGN853 treatment increases autophagic flux and cell death in FOLR1<sup>+</sup> ovarian high-grade serous carcinoma (HGSC) models. <b>A,</b> Representative confocal IF images showing expression patterns of the autophagy markers LC3B (green) beclin-1 control- (formulation buffer, top row) IMGN853-treated PDX tumors (bottom row). <b>B,</b> Fluorescence intensity each image was quantified using Imaris v. 9.1.2. The sum fluorescence for marker is...
<p>Combination therapy with IMGN853 and the PARP inhibitor olaparib in OVCAR-8 model. <b>A,</b> MTT assay results showing combinatorial effects of different concentrations IMGN853+olaparib on viability cells after 72 hours treatment [shown as log IMGN853(M)]. <i>n</i> = 3; data are mean ± SD. <b>B,</b> The CI plot for olaparib. <b>C,</b> Schematic representation mouse models established using ip2-luc (4 ×...
<p>Ovarian high-grade serous carcinomas (HGSCs) have heterogeneous FOLR1 expression. <b>A</b> and <b>B,</b> UMAP visualization of major cell types in five freshly resected tumors patients with HGSC [three primary (T) two omental metastases (OMT)] by sample type (<b>A</b>) (<b>B</b>). <b>C</b> <b>D,</b> expression epithelial cells after reclustering the level <b>E,</b> Representative IHC images tissue...
<p>Supplementary Figures S1 to S8</p>
<div>Abstract<p>Antibodies targeting folate receptor 1 (FOLR1) induce autophagic cell death in addition to antibody-dependent cytotoxicity, but the biological relevance of anti-FOLR1 antibody–induced autophagy for clinical applications remains unclear. In this study, we investigated role triggered by IMGN853 (mirvetuximab soravtansine), an FOLR1-targeted antibody–drug conjugate, and explored potential combinations with chemotherapeutic drugs used ovarian cancer treatment. We...
Loss of function in transport protein particles (TRAPP) links a new set emerging genetic disorders called "TRAPPopathies".One such disorder is NIBP syndrome, characterized by microcephaly and intellectual disability, caused mutations NIBP/TRAPPC9, crucial unique member TRAPPII.To investigate the neural cellular/molecular mechanisms underlying microcephaly, we developed Nibp/Trappc9-deficient animal models using different techniques, including morpholino knockdown CRISPR/Cas mutation...
Background: There have been cases of colorectal cancer (CRC) metastasizing into the ovary. This study reports a case involving solitary ovarian metastasis (OM) from CRC, which is very rare in absence other pelvic and peritoneal metastases. atypical clinical presentation added to complexity diagnosis. Case Description: We report OM-CRC 48-year-old woman. The patient underwent CRC surgery refused follow-up after three rounds chemotherapy. Approximately 14 months later, presented with vaginal...